Skip to main content
SpringerLink
Log in

Genetic mining of the “dark matter” in fungal natural products

  • Insight
  • Published:
Science China Life Sciences Aims and scope Submit manuscript

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

References

  • Bai, T., Quan, Z., Zhai, R., Awakawa, T., Matsuda, Y., and Abe, I. (2018). Elucidation and heterologous reconstitution of chrodrimanin B biosynthesis. Org Lett 20, 7504–7508.

    Article  CAS  PubMed  Google Scholar 

  • Blin, K., Shaw, S., Steinke, K., Villebro, R., Ziemert, N., Lee, S.Y., Medema, M.H., and Weber, T. (2019). antiSMASH 5.0: updates to the secondary metabolite genome mining pipeline. Nucleic Acids Res 47, W81–W87.

    Article  Google Scholar 

  • Chiang, Y.M., Ahuja, M., Oakley, C.E., Entwistle, R., Asokan, A., Zutz, C., Wang, C.C.C., and Oakley, B.R. (2016). Development of Genetic Dereplication Strains in Aspergillus nidulans Results in the Discovery of Aspercryptin. Angew Chem Int Ed 55, 1662–1665.

    Article  CAS  Google Scholar 

  • Clevenger, K.D., Bok, J.W., Ye, R., Miley, G.P., Verdan, M.H., Velk, T., Chen, C., Yang, K.H., Robey, M.T., Gao, P., et al. (2017). A scalable platform to identify fungal secondary metabolites and their gene clusters. Nat Chem Biol 13, 895–901.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Dohn, J.W., Grubbs, A.W., Oakley, C.E., and Oakley, B.R. (2018). New multi-marker strains and complementing genes for Aspergillus nidulans molecular biology. Fungal Genets Biol 111, 1–6.

    Article  CAS  Google Scholar 

  • Fan, J., Liao, G., Kindinger, F., Ludwig-Radtke, L., Yin, W.B., and Li, S.M. (2019). Peniphenone and Penilactone Formation in Penicillium crustosum via 1,4-Michael Additions of ortho-Quinone Methide from Hydroxyclavatol to γ-Butyrolactones from Crustosic Acid. J Am Chem Soc 141, 4225–4229.

    Article  CAS  PubMed  Google Scholar 

  • Guan, F., Pan, Y., Li, J., and Liu, G. (2017). A GATA-type transcription factor AcAREB for nitrogen metabolism is involved in regulation of cephalosporin biosynthesis in Acremonium chrysogenum. Sci China Life Sci 60, 958–967.

    Article  CAS  PubMed  Google Scholar 

  • Hai, Y., Huang, A.M., and Tang, Y. (2019). Structure-guided function discovery of an NRPS-like glycine betaine reductase for choline biosynthesis in fungi. Proc Natl Acad Sci USA 116, 10348–10353.

    Article  CAS  PubMed  Google Scholar 

  • Harvey, C.J.B., Tang, M., Schlecht, U., Horecka, J., Fischer, C.R., Lin, H. C., Li, J., Naughton, B., Cherry, J., Miranda, M., et al. (2018). HEx: A heterologous expression platform for the discovery of fungal natural products. Sci Adv 4, eaar5459.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • He, Y., Wang, B., Chen, W., Cox, R.J., He, J., and Chen, F. (2018). Recent advances in reconstructing microbial secondary metabolites biosynthesis in Aspergillus spp.. Biotech Adv 36, 739–783.

    Article  CAS  Google Scholar 

  • Itoh, H., Matsui, M., Miyamura, Y., Takeda, I., Ishii, J., Kumagai, T., Machida, M., Shibata, T., and Arita, M. (2018). Biosynthesis of Novel Statins by Combining Heterologous Genes from Xylaria and Aspergillus. ACS Synth Biol 7, 2783–2789.

    Article  CAS  PubMed  Google Scholar 

  • Jiang, W., Zhao, X., Gabrieli, T., Lou, C., Ebenstein, Y., and Zhu, T.F. (2015). Cas9-Assisted Targeting of CHromosome segments CATCH enables one-step targeted cloning of large gene clusters. Nat Commun 6, 8101.

    Article  PubMed  PubMed Central  Google Scholar 

  • Kang, H.S., Charlop-Powers, Z., and Brady, S.F. (2016). Multiplexed CRISPR/Cas9- and TAR-mediated promoter engineering of natural product biosynthetic gene clusters in Yeast. ACS Synth Biol 5, 1002–1010.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Keller, N.P. (2019). Fungal secondary metabolism: regulation, function and drug discovery. Nat Rev Microbiol 17, 167–180.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Li, R., Li, Z.X., Ma, K., Wang, G., Li, W., Liu, H.W., Yin, W.B., Zhang, P., and Liu, X.Z. (2019a). Strategy for efficient cloning of biosynthetic gene clusters from fungi. Sci China Life Sci 11.

  • Li, W., Fan, A., Wang, L., Zhang, P., Liu, Z., An, Z., and Yin, W.B. (2018). Asperphenamate biosynthesis reveals a novel two-module NRPS system to synthesize amino acid esters in fungi. Chem Sci 9, 2589–2594.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Li, W., Yu, J., Li, Z., and Yin, W.B. (2019b). Rational design for fungal laccase production in the model host Aspergillus nidulans. Sci China Life Sci 62, 84–94.

    Article  CAS  PubMed  Google Scholar 

  • Li, Y., and Tan, H. (2017). Biosynthesis and molecular regulation of secondary metabolites in microorganisms. Sci China Life Sci 60, 935–938.

    Article  CAS  PubMed  Google Scholar 

  • Lyu, H.N., Liu, H.W., Keller, N.P., and Yin, W.B. (2019). Harnessing diverse transcriptional regulators for natural product discovery in fungi. Nat Prod Rep 75.

  • Macheleidt, J., Mattern, D.J., Fischer, J., Netzker, T., Weber, J., Schroeckh, V., Valiante, V., and Brakhage, A.A. (2016). Regulation and role of fungal secondary metabolites. Annu Rev Genet 50, 371–392.

    Article  CAS  PubMed  Google Scholar 

  • Pfannenstiel B.T., Keller N.P. (2019). On top of biosynthetic gene clusters: How epigenetic machinery influences secondary metabolism in fungi. Biotechnol Adv S0734-9750(19)30012-6.

  • Qin, B., Matsuda, Y., Mori, T., Okada, M., Quan, Z., Mitsuhashi, T., Wakimoto, T., and Abe, I. (2016). An unusual chimeric diterpene synthase from Emericella variecolor and its functional conversion into a sesterterpene synthase by domain swapping. Angew Chem Int Ed 55, 1658–1661.

    Article  CAS  Google Scholar 

  • Skellam, E. (2019). Strategies for engineering natural product biosynthesis in fungi. Trends Biotech 37, 416–427.

    Article  CAS  Google Scholar 

  • van Dijk, J.W.A., Guo, C.J., and Wang, C.C.C. (2016). Engineering fungal nonribosomal peptide synthetase-like enzymes by heterologous expression and domain swapping. Org Lett 18, 6236–6239.

    Article  CAS  PubMed  Google Scholar 

  • Wang, C., Hantke, V., Cox, R.J., and Skellam, E. (2019). Targeted gene inactivations expose silent cytochalasans in Magnaporthe grisea NI980. Org Lett 21, 4163–4167.

    Article  CAS  PubMed  Google Scholar 

  • Zhang, J.J., Tang, X., and Moore, B.S. (2019). Genetic platforms for heterologous expression of microbial natural products. Nat Prod Rep 71.

  • Zhang, X., Lu, C., and Bai, L. (2017). Conversion of the high-yield salinomycin producer Streptomyces albus BK3-25 into a surrogate host for polyketide production. Sci China Life Sci 60, 1000–1009.

    Article  CAS  PubMed  Google Scholar 

  • Zheng, Y., Wang, X., Zhang, X., Li, W., Liu, G., Wang, S., Yan, X., Zou, H., and Yin, W.B. (2017). COP9 signalosome subunit PfCsnE regulates secondary metabolism and conidial formation in Pestalotiopsis fici. Sci China Life Sci 60, 656–664.

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgements

This work was supported by the National Key Research and Development Program of China (2018YFD0400200), the National Natural Science Foundation of China (31861133004), and the Biological Resources Program, Chinese Academy of Sciences (KFJ-BRP-009). We thank Mrs. Jie Fan (University of Marburg) for critically reading and discussing the manuscript.

Author information

Authors and Affiliations

  1. State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China

    Wei Li & Wen-Bing Yin

  2. Savaid Medical School, University of Chinese Academy of Sciences, Beijing, 100049, China

    Wei Li & Wen-Bing Yin

Authors
  1. Wei Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Wen-Bing Yin
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Wen-Bing Yin.

Ethics declarations

Compliance and ethics The author(s) declare that they have no conflict of interest.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Li, W., Yin, WB. Genetic mining of the “dark matter” in fungal natural products. Sci. China Life Sci. 62, 1250–1252 (2019). https://doi.org/10.1007/s11427-019-9818-3

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11427-019-9818-3

Search

Navigation