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Overexpression of the diguanylate cyclase CdgD blocks developmental transitions and antibiotic biosynthesis in Streptomyces coelicolor

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Abstract

Cyclic dimeric GMP (c-di-GMP) has emerged as the nucleotide second messenger regulating both development and antibiotic production in high-GC, Gram-positive streptomycetes. Here, a diguanylate cyclase (DGC), CdgD, encoded by SCO5345 from the model strain Streptomyces coelicolor, was functionally identified and characterized to be involved in c-di-GMP synthesis through genetic and biochemical analysis. cdgD overexpression resulted in significantly reduced production of actinorhodin and undecylprodigiosin, as well as completely blocked sporulation or aerial mycelium formation on two different solid media. In the cdgD-overexpression strain, intracellular c-di-GMP levels were 13-27-fold higher than those in the wild-type strain. In vitro enzymatic assay demonstrated that CdgD acts as a DGC, which could efficiently catalyze the synthesis of c-di-GMP from two GTP molecules. Heterologous overproduction of cdgD in two industrial Streptomyces strains could similarly impair developmental transitions as well as antibiotic biosynthesis. Collectively, our results combined with previously reported data clearly demonstrated that c-di-GMP-mediated signalling pathway plays a central and universal role in the life cycle as well as secondary metabolism in streptomycetes.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (31630003, 31570072 and 31770088), the Science and Technology Commission of Shanghai Municipality (18ZR1446700) and the National Science and Technology Major Project (2017ZX09101003-006-012). We are grateful to Yuanhong Shan, Wenli Hu and Wenzhi Zhou for LC-MS/MS analysis. We also thank Zhiping Zhang and Jiqin Li for field emission scanning electron microscopy (FESEM) analysis.

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Authors and Affiliations

  1. Key Laboratory of Synthetic Biology, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai, 200032, China

    Xiaocao Liu, Guosong Zheng, Weihong Jiang & Lei Li

  2. School of Life Sciences, Henan University, Kaifeng, 475004, China

    Xiaocao Liu & Gang Wang

  3. Jiangsu National Synergetic Innovation Center for Advanced Materials, SICAM, Nanjing, 210009, China

    Weihong Jiang

  4. School of Life Sciences, Shanghai Normal University, Shanghai, 200232, China

    Yinhua Lu

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  1. Xiaocao Liu
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  2. Guosong Zheng
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  5. Lei Li
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  6. Yinhua Lu
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Correspondence to Lei Li or Yinhua Lu.

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Compliance and ethics The author(s) declare that they have no conflict of interest.

Supporting Information

Figure S1 Verification of the deletion of the six tested c-di-GMP-metabolizing enzyme genes in S. coelicolor by colony PCR.

Figure S2 Effects of the deletion of the six predicted c-di-GMP-metabolizing enzyme genes on morphological differentiation and antibiotic biosynthesis in S. coelicolor.

Figure S3 Time-course transcriptional analysis of the six predicted c-di-GMP-metabolizing genes in the wild-type S. coelicolor M145 on solid MS (A) and R2YE (B) media.

Figure S4 Effects of overexpression of the six predicted c-di-GMP-metabolizing enzyme genes on morphological differentiation and antibiotic biosynthesis in S. coelicolor on solid MS and R2YE media.

Figure S5 Intracellular c-di-GMP levels in the cdgC-overexpression mutant.

Figure S6 Amino acid sequence alignment of the GGDEF domains from eight identified or predicted c-di-GMP-metabolizing enzymes.

Figure S7 Some clues for the regulation of CdgD expression in S. coelicolor.

Table S1 Bacterial strains and plasmids used in this study

Table S2 Oligonucleotide sequences used in this study

The supporting information is available online at http://life.scichina.com and https://link.springer.com. The supporting materials are published as submitted, without typesetting or editing. The responsibility for scientific accuracy and content remains entirely with the authors.

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11427_2019_9549_MOESM1_ESM.doc

Overexpression of the diguanylate cyclase CdgD blocks developmental transitions and antibiotic biosynthesis in Streptomyces coelicolor

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Liu, X., Zheng, G., Wang, G. et al. Overexpression of the diguanylate cyclase CdgD blocks developmental transitions and antibiotic biosynthesis in Streptomyces coelicolor. Sci. China Life Sci. 62, 1492–1505 (2019). https://doi.org/10.1007/s11427-019-9549-8

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