Science China Life Sciences

, Volume 62, Issue 8, pp 1070–1077 | Cite as

A transient expression system in soybean mesophyll protoplasts reveals the formation of cytoplasmic GmCRY1 photobody-like structures

  • Lu Xiong
  • Cong Li
  • Hongyu Li
  • Xiangguang Lyu
  • Tao Zhao
  • Jun Liu
  • Zecheng ZuoEmail author
  • Bin LiuEmail author
Research Paper


Soybean (Glycine max (L.) Merr.), grown for its plant oils and proteins, is one of the most important crops throughout the world. Generating stable and heritable transgenic soybeans is relatively inefficient; therefore, there is an urgent need for a simple and high-efficient transient transformation method by which to enable the investigation of gene functions in soybeans, which will facilitate the elucidation and improvement of the molecular mechanisms regulating the associated agronomic traits. We established a system of transient expression in soybean mesophyll protoplasts and obtained a high level of protoplast transfection efficiency (up to 83.5%). The subcellular activity of the protoplasts was well preserved, as demonstrated by the dynamic formation of GmCRY nucleus photobodies (NPs) and/or cytoplasmic photobody-like structures (CPs) in response to blue light. In addition, we showed that GmCRY1b CPs colocalized with GmCOP1b, a co-ortholog of Arabidopsis thaliana CONSTITUTIVE PHOTOMORPHOGENIC 1 (COP1), which provided new insight into the potential roles of GmCRY1s in the cytoplasm.


soybean protoplast transfection subcellular localization cryptochrome photobodies 


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This work was supported by the National Key Research and Development Plan (2016YFD0101005), the National Natural Science Foundation of China (31871705 and 31422041), and the Central Public-Interest Scientific Institution Basal Research Fund (Y2016JC13).

Supplementary material

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Supplementary material, approximately 2.61 MB.


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Copyright information

© Science China Press and Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijingChina
  2. 2.Jilin Province Engineering Laboratory of Plant Genetic Improvement, College of Plant ScienceJilin UniversityChangchunChina

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