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Science China Life Sciences

, Volume 62, Issue 8, pp 1019–1027 | Cite as

Macleaya cordata extract alleviated oxidative stress and altered innate immune response in mice challenged with enterotoxigenic Escherichia coli

  • Guiping Guan
  • Sujuan Ding
  • Yulong Yin
  • Veeramuthu Duraipandiyan
  • Naif Abdullah Al-Dhabi
  • Gang LiuEmail author
Research Paper
  • 31 Downloads

Abstract

This study examines the effects of dietary Macleaya cordata extract (MCE) on bacterial burden and resistance to enterotoxigenic Escherichia coli (ETEC) in ICR mice. ICR mice were randomly distributed into one of the following groups: (i) basal diet; (ii) basal diet with 200 mg kg−1 MCE; (iii) basal diet challenged with ETEC; and (iv) basal diet with 200 mg kg−1 MCE and challenged with ETEC. Following a 7-day period of pre-treatment, CTRL-ETEC and MCE-ETEC mice were subjected to oral infection using 5×108E. coli SEC 470. The results showed dietary 200 mg kg−1 MCE markedly reduced intestinal ETEC burden (P < 0.05) and the disease-associated mortality was significantly alleviated in the MCE treated group (P < 0.05). In addition, dietary MCE markedly alleviated ETEC-induced oxidative stress, evidenced by the lowered methane dicarboxylic aldehyde (MDA) abundance and enhanced activities of catalase and glutathione peroxidase (P < 0.05). Furthermore, MCE mice exhibited higher immune activity, which might have further mediated ETEC infection. These results indicate MCE plays a preventative role with respect to ETEC infection. Future research should aim to develop MCE as a therapeutic approach to the promotion of intestinal health and a safeguard against ETEC infection.

Macleaya cordata extract enterotoxigenic Escherichia coli bacterial burden oxidative stress 

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Notes

Acknowledgements

This research was supported by the National Natural Science Foundation of China (31772642, 31402092), the China Scholarship Council (201708430008), Scientific Research Fund of Hunan Provincial Education Department (17K043), Hunan Provincial Science and Technology Department (2017NK2322, 2018TP2031), China Postdoctoral Science Foundation (2018M632963), Double First-class Construction Project of Hunan Agricultural University (SYL201802002). The authors would like to extend their sincere appreciation to the Deanship of Scientific Research at King Saud University for its funding of this research through the Research Group Project No. RGP-213.

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Copyright information

© Science China Press and Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Guiping Guan
    • 1
  • Sujuan Ding
    • 1
  • Yulong Yin
    • 2
    • 3
  • Veeramuthu Duraipandiyan
    • 4
  • Naif Abdullah Al-Dhabi
    • 4
  • Gang Liu
    • 1
    • 3
    Email author
  1. 1.College of Bioscience and BiotechnologyHunan Agricultural UniversityChangshaChina
  2. 2.College of Animal ScienceSouth China Agricultural UniversityGuangzhouChina
  3. 3.Hunan Province Key Laboratory of Animal Nutritional Physiology and Metabolic Process, Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical AgricultureChinese Academy of Sciences, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry ProductionChangshaChina
  4. 4.Department of Botany and Microbiology, College of ScienceKing Saud UniversityRiyadhSaudi Arabia

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