Skip to main content

Structural and functional insights into MCR-2 mediated colistin resistance

This is a preview of subscription content, access via your institution.


  • AbuOun, M., Stubberfield, E.J., Duggett, N.A., Kirchner, M., Dormer, L., Nunez-Garcia, J., Randall, L.P., Lemma, F., Crook, D.W., Teale, C., et al. (2017). mcr-1 and mcr-2 (mcr-6.1) variant genes identified in Moraxella species isolated from pigs in Great Britain from 2014 to 2015. J Antimicrobial Chemother 72, 2745–2749.

    Article  CAS  Google Scholar 

  • Anandan, A., Evans, G.L., Condic-Jurkic, K., O’Mara, M.L., John, C.M., Phillips, N.J., Jarvis, G.A., Wills, S.S., Stubbs, K.A., Moraes, I., et al. (2017). Structure of a lipid A phosphoethanolamine transferase suggests how conformational changes govern substrate binding. Proc Natl Acad Sci USA 114, 2218–2223.

    Article  PubMed  CAS  Google Scholar 

  • Coates, K., Walsh, T.R., Spencer, J., and Hinchliffe, P. (2017). 1.12 Å resolution crystal structure of the catalytic domain of the plasmid-mediated colistin resistance determinant MCR-2. Acta Crystlogr F Struct Biol Commun 73, 443–449.

    Article  CAS  Google Scholar 

  • Gao, R., Li, Y., Lin, J., Tan, C., and Feng, Y. (2016). Unexpected complexity of multidrug resistance in the mcr-1-harbouring Escherichia coli. Sci China Life Sci 59, 732–734.

    Article  PubMed  Google Scholar 

  • Garcia-Graells, C., De Keersmaecker, S.C.J., Vanneste, K., Pochet, B., Vermeersch, K., Roosens, N., Dierick, K., and Botteldoorn, N. (2018). Detection of plasmid-mediated colistin resistance, mcr-1 and mcr-2 genes, in Salmonella spp. isolated from food at retail in Belgium from 2012 to 2015. Foodborne Pathog Dis 15, 114–117.

    Article  PubMed  CAS  Google Scholar 

  • Hinchliffe, P., Yang, Q.E., Portal, E., Young, T., Li, H., Tooke, C.L., Carvalho, M.J., Paterson, N.G., Brem, J., Niumsup, P.R., et al. (2017). Insights into the mechanistic basis of plasmid-mediated colistin resistance from crystal structures of the catalytic domain of MCR-1. Sci Rep 7, 39392.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Hu, Y., Liu, F., Lin, I.Y., Gao, G.F., and Zhu, B. (2016). Dissemination of the mcr-1 colistin resistance gene. Lancet Infect Dis 16, 146–147.

    Article  PubMed  Google Scholar 

  • Liu, Y.Y., Wang, Y., Walsh, T.R., Yi, L.X., Zhang, R., Spencer, J., Doi, Y., Tian, G., Dong, B., Huang, X., et al. (2016). Emergence of plasmidmediated colistin resistance mechanism MCR-1 in animals and human beings in China: a microbiological and molecular biological study. Lancet Infect Dis 16, 161–168.

    Article  PubMed  CAS  Google Scholar 

  • Manohar, P., Shanthini, T., Ayyanar, R., Bozdogan, B., Wilson, A., Tamhankar, A.J., Nachimuthu, R., and Lopes, B.S. (2017). The distribution of carbapenem- and colistin-resistance in Gram-negative bacteria from the Tamil Nadu region in India. J Med Microbiol 66, 874–883.

    Article  PubMed  CAS  Google Scholar 

  • Peleg, A.Y., and Hooper, D.C. (2010). Hospital-acquired infections due to Gram-negative bacteria REPLY. New Engl J Med 363, 1483–1484.

    CAS  Google Scholar 

  • Stojanoski, V., Sankaran, B., Prasad, B.V.V., Poirel, L., Nordmann, P., and Palzkill, T. (2016). Structure of the catalytic domain of the colistin resistance enzyme MCR-1. BMC Biol 14, 81.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Sun, J., Xu, Y., Gao, R., Lin, J., Wei, W., Srinivas, S., Li, D., Yang, R.S., Li, X.P., Liao, X.P., et al. (2017). Deciphering MCR-2 colistin resistance. mBio 8, e00625–17.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Trott, O., and Olson, A.J. (2010). AutoDock Vina: improving the speed and accuracy of docking with a new scoring function, efficient optimization, and multithreading. J Comput Chem 31, 455–461.

    PubMed  PubMed Central  CAS  Google Scholar 

  • Xavier, B.B., Lammens, C., Ruhal, R., Kumar-Singh, S., Butaye, P., Goossens, H., and Malhotra-Kumar, S. (2016). Identification of a novel plasmid-mediated colistin-resistance gene, mcr-2, in Escherichia coli, Belgium, June 2016. Eurosurveillance 21, 8–13.

    Article  Google Scholar 

  • Ye, H., Li, Y., Li, Z., Gao, R., Zhang, H., Wen, R., Gao G.F., Hu, Q., and Feng, Y. (2016). Diversified mcr-1-harbouring plasmid reservoirs confer resistance to colistin in human gut microbiota. mBio 7, e00177.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

Download references


We are grateful to Dr Gary Wong, Hao Song, Shuguang Tan and Lifeng Fu for helping prepare the figures and revise the manuscript. This work was supported by the Chinese Academy of Sciences (CAS) Strategic Priority Research Program (XDB08020100), the External Cooperation Program of Chinese Academy of Sciences (153211KYSB20160001) and the National Key Plan for Scientific Research and Development of China (2016YFD0500305). George Fu Gao is a leading principal investigator of the National Natural Science Foundation of China (NSFC) Innovative Research Group (81621091). The atomic coordinates and structure factors have been deposited in the Protein Data Bank with PDB ID code 6A7W. X-ray data were collected at beam line 17U of the Shanghai Synchrotron Radiation Facility (SSRF).

Author information

Authors and Affiliations


Corresponding author

Correspondence to George Fu Gao.

Electronic supplementary material

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Wang, X., Lu, Q., Qi, J. et al. Structural and functional insights into MCR-2 mediated colistin resistance. Sci. China Life Sci. 61, 1432–1436 (2018).

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: