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Science China Life Sciences

, Volume 61, Issue 4, pp 457–463 | Cite as

Preparation and characterization of sulfonated chitosan-modified gold nanoparticles and their surface electronic payload of charged drugs

  • Huaisong Wang
  • Jia He
  • Ya Ding
  • Xinghua Xia
Research Paper
  • 42 Downloads

Abstract

Chitosan (CS), a kind of naturally produced polysaccharide with extraordinary biocompatibility and biodegradation, shows much potential to act as reducing and stabilizing agent in the synthesis of gold nanoparticles (AuNPs) for drug delivery. To solve the poor solubility and expand the pharmaceutical applications of CS, various CS derivatives through rational design have been developed and further used to prepare, stabilize, and mediate self-assembling of gold materials. Herein, we chose sulfonic chitosan as a stabilizing reagent for the synthesis of highly stable AuNPs (AuNP/SCSs) with diameters of about 3 nm. For investigating their surface electronic payload of charged drugs, the negatively charged fluorescence isothiocyanate (FITC) and positively charged Rhodamine B (Rb) were used as models to be modified on the surface of the AuNP/SCSs via a layer-by-layer (LbL) method. With a basis of the fluorescence resonance energy transfer (FRET) principle, via adjusting the distance between AuNPs and fluorescent molecules by tuning the layers of charged polymers, the regulation of the fluorescence intensity of the fluorescent molecules has been achieved. In addition, the drug loading efficiency was investigated.

Keywords

sulfonic chitosan gold nanoparticle layer-by-layer (LbL) self-assembly fluorescent molecule 

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Notes

Acknowledgements

This work was supported by the National Natural Science Foundation of China (30900337, 31470916, 81673390), Jiangsu Provincial Natural Science Foundation (BK20150689), the Fundamental Research Funds for the Central Universities (2015PT036), the Priority Academic Program Development of Jiangsu Higher Education Institutions and the Open Project Program of MOE Key Laboratory of Drug Quality Control and Pharmacovigilance (DQCP2015MS01).

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Copyright information

© Science China Press and Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Key Laboratory of Drug Quality Control and Pharmacovigilance, China Pharmaceutical UniversityMinistry of EducationNanjingChina
  2. 2.State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical EngineeringNanjing UniversityNanjingChina

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