Interplay of transcription factors and microRNAs during embryonic hematopoiesis
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Hematopoietic stem cells (HSCs), which are localized in the bone marrow of adult mammals, come from hematopoietic endothelium during embryonic stages. Although the basic processes of HSC generation and differentiation have been described in the past, the epigenetic regulation of embryonic hematopoiesis remains to be fully described. Here, by utilizing an in vitro differentiation system of mouse embryonic stem cells (ESCs), we identified more than 20 microRNAs that were highly enriched in embryonic hematopoietic cells, including some (e.g. miR-10b, miR-15b, and miR-27a) with previously unknown functions in blood formation. Luciferase and gene expression assays further revealed combinational binding and regulation of these microRNAs by key transcription factors in blood cells. Finally, bioinformatics and functional analyses supported an interactive regulatory control between transcription factors and microRNAs in hematopoiesis.
Keywordshematopoiesis embryonic stem cells microRNA
The microarray work was performed at the sequence core facility of National Institute of Environmental Health Sciences (NIEHS). This study was supported by the Ministry of Science and Technology of China (2016YFA0100302, 2014CB964800), the National Natural Science Foundation of China (31471347, 30971522, 31271589), the Science and Technology Commission of Shanghai Municipality (11DZ2260300, 13JC1406402, 16JC1404200).
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