Soshiho-tang protects LPS-induced acute liver injury by attenuating inflammatory response


Soshiho-tang (SSHT) has traditionally been used to treat gastrointestinal disorders. In this experiment, we investigated the protective effect of SSHT on inflammatory liver injury in lipopolysaccharide (LPS)-sensitized mice. Male C57BL/6J mice aged 6 weeks were randomly placed in 6 groups (n = 5): normal mice (CTR), LPS-sensitized mice (LPS), LPS-sensitized mice treated with dexamethasone (DEX) and LPS-sensitized mice treated with 0.05, 0.55, and 5.55 g/kg of SSHT (SSHT 0.05, SSHT 0.55, and SSHT 5.55). Various doses of SSHT was given once a day for 7 days. After 2 h of LPS injection, the liver tissue was collected. SSHT pretreatment recovered hemorrhage of liver tissues in LPS-induced acute liver injury. The expressions of MAP Kinase, NF-κB, IκBα, p-IκBα, COX-2, and iNOS protein levels were markedly decreased by SSHT-treated liver tissues. Additionally, SSHT pretreatment significantly regulated the expressions of MCP-1, TNF-α, and IL-6 cytokines. These results suggest the potential of SSHT on the protection of acute liver injury.

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This work was supported by a grant of the National Development Institute of Korean Medicine (NIKOM) funded by the Korean Ministry of Health and Welfare (MOHW), Republic of Korea, and a National Research Foundation of Korea Grant funded by the Korean government (NRF-2019R1I1A2A01063598).

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Correspondence to Woong Mo Yang.

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Jin, S.C., Kim, M.H., Jo, S.Y. et al. Soshiho-tang protects LPS-induced acute liver injury by attenuating inflammatory response. J Nat Med (2020).

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  • Soshiho-tang
  • Liver inflammation
  • LPS
  • Acute liver injury
  • Anti-inflammation