Caffeic acid and rosmarinic acid contents in genus Perilla

Abstract

Caffeic acid and rosmarinic acid are common components of Labiatae plants, such as shiso (Perilla frutescens Britton var. crispa W. Deane) and Boraginaceae plants. These compounds have various pharmacological activities, such as anti-inflammatory, anti-anxiety, and anti-depressive activities, but the content of these compounds in perilla has not been studied in detail. This study investigated the caffeic acid and rosmarinic acid contents of several pure strains in genus Perilla. Perilla plants cultivated under a certain set of conditions had different caffeic acid and rosmarinic acid contents. For example, their contents were higher in P. setoyensis (“Setoegoma”), suggesting that the genetic background of the species greatly affects caffeic acid and rosmarinic acid contents. Several strains of P. frutescens var. crispa were cultivated at the Experimental Station for Medicinal Plants, Graduate School of Pharmaceutical Sciences, Kyoto University and differences in their caffeic acid and rosmarinic acid contents were also observed. The total content of anthocyanins, which are closely related to the leaf color of perilla, was measured as cyanidin-3-glucoside equivalents, and a weak positive correlation was observed between the content of rosmarinic acid, and the total content of total anthocyanins. Furthermore, the results suggest that luminosity and photon flux density of light during cultivation can affect rosmarinic acid content.

This is a preview of subscription content, log in to check access.

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

References

  1. 1.

    Deguchi Y, Ito M (2020) Rosmarinic acid in Perilla frutescens and perilla herb analyzed by HPLC. J Nat Med 74:341–352

    Article  Google Scholar 

  2. 2.

    Mudgal J, Mallik SB, Nampoothiri M, Kinra M, Hall S, Grant GD, Anoopkumar-Dukie S, Davey AK, Rao CM, Arora D (2020) Effect of coffee constituents, caffeine and caffeic acid on anxiety and lipopolysaccharide-induced sickness behavior in mice. J Funct Foods 64:103638

    Article  Google Scholar 

  3. 3.

    Takeda H, Tsuji M, Inazu M, Egashira T, Matsumiya T (2002) Rosmarinic acid and caffeic acid produce antidepressive-like effect in the forced swimming test in mice. Eur J Pharmacol 449:261–267

    Article  Google Scholar 

  4. 4.

    Kanemaki A, Otani M, Takano M, Fujimoto T, Okuhara H, Nomizu T, Kondo M, Kobayashi H, Tatsuzawa F, Nakano M (2018) Ectopic expression of the R2R3-MYB gene from Tricyrtis sp. Results in leaf color alteration in transgenic Pelargonium crispum. Sci Hortic 240:411–416

    Article  Google Scholar 

  5. 5.

    The Ministry of Health, Labour and Welfare (2016) The Japanese pharmacopoeia, 17th edn. The Ministry of Health, Labour and Welfare, Tokyo, pp 1843–1844

    Google Scholar 

  6. 6.

    Yamamoto H, Sakakibara J, Nagatsu A, Sekiya K (1998) Inhibitors of arachidonate lipoxygenase from defatted perilla seed. J Agric Food Chem 46:862–865

    Article  Google Scholar 

  7. 7.

    Sanbongi C, Takano H, Osakabe N, Sasa N, Natsume M, Yanagisawa R, Inoue K-I, Sadakane K, Ichinose T, Yoshikawa T (2004) Rosmarinic acid in perilla extract inhibits allergic inflammation induced by mite allergen, in a mouse model. Clin Exp Allergy 34:971–977

    Article  Google Scholar 

  8. 8.

    Takeda H, Tsuji M, Miyamoto J, Matsumiya T (2002) Rosmarinic acid and caffeic acid reduce the defensive freezing behavior of mice exposed to conditioned fear stress. Psychopharmacology 164:233–235

    Article  Google Scholar 

  9. 9.

    Lu N, Bernardo EL, Tippayadarapanich T, Takagaki M, Kagawa N, Yamori W (2017) Growth and accumulation of secondary metabolites in perilla as affected by photosynthetic photon flux density and electrical conductivity of the nutrient solution. Front Plant Sci 8(708):1–12

    Google Scholar 

Download references

Author information

Affiliations

Authors

Corresponding author

Correspondence to Michiho Ito.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Deguchi, Y., Ito, M. Caffeic acid and rosmarinic acid contents in genus Perilla. J Nat Med (2020). https://doi.org/10.1007/s11418-020-01418-5

Download citation

Keywords

  • Perilla frutescens
  • Caffeic acid
  • Rosmarinic acid
  • Anthocyanins