A search for bioactive natural products as anticancer lead compounds resulted in the isolation of one previously undescribed and three known clerodane diterpenoids (1–4) from Casearia kurzii. The structures of these compounds were established by analysis of their NMR, MS, and electronic circular dichroism data. The cytotoxic activities of four compounds against three human cancer cell lines were evaluated. Compound 2 was found to be the most active with an IC50 value of 4.1 μM against HeLa cells, and was selected to investigate the possible cytotoxic mechanism.
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This work was supported by the National University Student Innovation Program of Nankai University, the National Natural Science Foundation of China (No. U1703107), the Natural Science Foundation of Tianjin, China (No. 16JCYBJC27700), and Hundred Young Academic Leaders Program of Nankai University.
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Appendix A: Supplementary data. 1D and 2D NMR spectra of are available as supplementary data. Supplementary data related to this article can be found online. 1 (DOC 231 kb)
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Shuo, Y., Zhang, C., Yang, X. et al. Clerodane diterpenoids from Casearia kurzii and their cytotoxic activities. J Nat Med 73, 826–833 (2019). https://doi.org/10.1007/s11418-019-01324-5
- Casearia kurzii
- Clerodane diterpenoids
- Cytotoxic activities
- Cell cycle