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Changes in content of triterpenoids and polysaccharides in Ganoderma lingzhi at different growth stages

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Abstract

Ganoderma lingzhi is a traditional medicinal mushroom, and its extract contains many bioactive compounds. Triterpenoids and polysaccharides are the primary bioactive components that contribute to its medicinal properties. In this study, we quantified 18 triterpenoids, total triterpenoid content and total polysaccharide content in the ethanol and water extracts of G. lingzhi at different growth stages. Triterpenoids were quantified by liquid chromatograph–tandem mass spectrometry in the multiple-reaction-monitoring mode. Total triterpenoid and total polysaccharide content were determined by colorimetric analysis. The results indicated that the fruit bodies at an early growth stage had a higher content of ganoderic acid A, C2, I and LM2, as well as of ganoderenic acid C and D, than those at a later growth stage. In contrast, ganoderic acid K, TN and T–Q contents were higher in mature fruit bodies (maturation stage). The highest total triterpenoid and total polysaccharide contents were found in fruit bodies before maturity (stipe elongation stage or early stage of pileus formation). Our results provide information which will contribute to the establishment of an efficient cultivation system for G. lingzhi with a higher content of triterpenoids.

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Acknowledgements

We appreciate the technical assistance from the Center for Advanced Instrumental and Educational Supports, Faculty of Agriculture, Kyushu University. This work was supported by JSPS KAKENHI Grant number JP17H03845.

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Authors and Affiliations

  1. Faculty of Agriculture, Kyushu University, Fukuoka, 812-8581, Japan

    Toshinori Nakagawa, Qinchang Zhu, Sonam Tamrakar, Yhiya Amen & Kuniyoshi Shimizu

  2. Department of Pharmacy, Shenzhen University, Shenzhen, 3688, China

    Qinchang Zhu

  3. Faculty of Pharmacy, Mansoura University, Mansoura, 35516, Egypt

    Yhiya Amen

  4. Department of Biomass Utilization, Institute of Agricultural and Forest Resources, Fukuoka Agriculture and Forestry Research Center, Fukuoka, 839-0827, Japan

    Yasuhiro Mori

  5. Miyazaki Prefectural Wood Utilization Research Center, Miyazaki, 880-8501, Japan

    Hiroto Suhara

  6. Fukuoka Prefecture Forest Research & Extension Center, Fukuoka, 839-0827, Japan

    Shuhei Kaneko

  7. Chemicals Evaluation and Research Institute, Fukuoka, 839-0801, Japan

    Hiroko Kawashima, Kotaro Okuzono & Yoshiyuki Inoue

  8. Department of Biological and Environmental Chemistry, Kindai University, Fukuoka, 820-8555, Japan

    Koichiro Ohnuki

Authors
  1. Toshinori Nakagawa
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  2. Qinchang Zhu
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  3. Sonam Tamrakar
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  4. Yhiya Amen
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  5. Yasuhiro Mori
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  6. Hiroto Suhara
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  7. Shuhei Kaneko
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  8. Hiroko Kawashima
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  9. Kotaro Okuzono
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  10. Yoshiyuki Inoue
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  11. Koichiro Ohnuki
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  12. Kuniyoshi Shimizu
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Corresponding author

Correspondence to Kuniyoshi Shimizu.

Additional information

Shuhei Kaneko formerly worked in Fukuoka Prefecture Forest Research & Extension Center.

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Nakagawa, T., Zhu, Q., Tamrakar, S. et al. Changes in content of triterpenoids and polysaccharides in Ganoderma lingzhi at different growth stages. J Nat Med 72, 734–744 (2018). https://doi.org/10.1007/s11418-018-1213-y

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  • DOI: https://doi.org/10.1007/s11418-018-1213-y

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