Journal of Natural Medicines

, Volume 71, Issue 4, pp 757–764 | Cite as

Effect of ninjin’yoeito and ginseng extracts on oxaliplatin-induced neuropathies in mice

  • Toshiaki Suzuki
  • Ayano Yamamoto
  • Masahiro Ohsawa
  • Yoshiharu Motoo
  • Hajime Mizukami
  • Toshiaki Makino
Original Paper


Oxaliplatin (L-OHP) is a platinum-based anticancer agent used to treat various types of cancer. It frequently causes acute and chronic peripheral neuropathies, such as cold allodynia and mechanical hyperalgesia. Ninjin’yoeito (NYT) is a formula used in traditional Japanese Kampo medicine to improve recovery from diseases and other medical disorders. We previously reported that treatment with a boiling-water extract of NYT prevented L-OHP-induced damage to neurite-like outgrowths from differentiated PC12 cells. The objectives of the present study were to evaluate the in vivo effects of NYT on L-OHP-induced neuropathic pain in mice and identify the active ingredients in NYT. Treatment with NYT extract significantly ameliorated both cold allodynia and mechanical hyperalgesia induced by L-OHP. While L-OHP treatment suppressed neurite outgrowths from primary dorsal root ganglion cells in vitro, NYT extract blocked this suppression in a concentration-dependent manner. Among the herbal components of NYT, the extract of ginseng (Panax ginseng roots) showed a protective effect against neurite damage induced by L-OHP, and one of its active ingredients was identified as ginsenoside Rg3. Ginseng extract partially relieved L-OHP-induced neuropathic pain in mice. Our results suggest that NYT could be an attractive agent for treating L-OHP-induced neuropathic pain, and that the active ingredient of NYT may be ginseng.


Oxaliplatin Neuropathy Ninjin’yoeito Root of Panax ginseng Ginsenoside Rg3 



This research was supported by Grant-in-Aid for Scientific Research (KAKENHI) C #16K09267 (2016), and the Kidani Memorial Trust (2016).


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Copyright information

© The Japanese Society of Pharmacognosy and Springer Japan KK 2017

Authors and Affiliations

  • Toshiaki Suzuki
    • 1
    • 2
  • Ayano Yamamoto
    • 1
  • Masahiro Ohsawa
    • 3
  • Yoshiharu Motoo
    • 4
  • Hajime Mizukami
    • 1
    • 5
  • Toshiaki Makino
    • 1
  1. 1.Department of Pharmacognosy, Graduate School of Pharmaceutical SciencesNagoya City UniversityNagoyaJapan
  2. 2.Department of Cellular Pharmacology, Graduate School of Pharmaceutical SciencesNagoya UniversityNagoyaJapan
  3. 3.Department of NeuropharmacologyGraduate School of Pharmaceutical SciencesNagoyaJapan
  4. 4.Department of Medical OncologyKanazawa Medical UniversityUchinadaJapan
  5. 5.The Kochi Prefectural Makino Botanical GardenKochiJapan

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