Journal of Natural Medicines

, Volume 71, Issue 4, pp 703–710 | Cite as

An overview and comparison of a recombinant antigen-binding fragment and an antigen-binding fragment from a monoclonal antibody against wogonin glucuronide

  • Madan Kumar Paudel
  • Seiichi Sakamoto
  • Hiroyuki Tanaka
  • Satoshi Morimoto
Original Paper


Wogonin glucuronide (wogonin 7-O-β-d-glucuronide, Wgn) is widely recognized as a constituent of Scutellariae radix, which is used in Kampo medicines. Wgn has been used for both pharmacological (antifebrile uses and in detoxification) and research purposes. A recombinant antigen-binding fragment (rFab) and an antigen-binding fragment from a monoclonal antibody (mFab) against Wgn were constructed and used in an indirect competitive enzyme-linked immunosorbent assay (icELISA) in this study. The rFab and mFab against Wgn showed both activity and recognition against Wgn. The developed icELISA was validated as a quantitative analytical method to detect Wgn by testing both its utility and its reliability using multiple concentrations of Wgn from S. radix. This approach provides a more economic method to analyze and purify Kampo medicines.


Wogonin glucuronide (Wgn) Recombinant antigen-binding fragment (rFab) Indirect competitive enzyme-linked immunosorbent assay (icELISA) Anti-Wgn monoclonal antibody (MAb 315A) Scutellariae radix Scutellaria baicalensis 



This study was supported, in part, by a Grant-in-Aid from KAKENHI, grant number 16K08296, and the research fund of Kyushu University Foundation.


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Copyright information

© The Japanese Society of Pharmacognosy and Springer Japan KK 2017

Authors and Affiliations

  • Madan Kumar Paudel
    • 1
  • Seiichi Sakamoto
    • 1
  • Hiroyuki Tanaka
    • 1
  • Satoshi Morimoto
    • 1
  1. 1.Department of Pharmacognosy, Graduate School of Pharmaceutical SciencesKyushu UniversityFukuokaJapan

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