Journal of Natural Medicines

, Volume 71, Issue 4, pp 650–658 | Cite as

Phytochemical profile and angiotensin I converting enzyme (ACE) inhibitory activity of Limonium michelsonii Lincz

  • Janar Jenis
  • Jeong Yoon Kim
  • Zia Uddin
  • Yeong Hun Song
  • Hyeong-Hwan Lee
  • Ki Hun Park
Original Paper


Members of the genus Limonium are widely used as medicinal herbs due to their health-promoting effects, such as an ability to improve blood circulation by inhibiting angiotensin I converting enzyme (ACE). While the potential of L. michelsonii Lincz. (a medicinal plant endemic to Kazakhstan) to inhibit ACE has been demonstrated, the inhibitory activities of its secondary metabolites have not been explored. In this work, the principal phenolic compounds (120) among these metabolites were isolated to determine the components responsible for ACE inhibition. The natural abundances of the active constituents within the target plant were characterized by UPLC-Q-TOF/MS analysis. All of the isolated compounds except for gallates 1012 were found to significantly inhibit ACE, with IC50 values of between 7.1 and 138.4 μM. Unexpectedly, the flavonol glycosides 1620 were observed to be more potent than the corresponding aglycones 4 and 5. For example, quercetin (4) had IC50 = 30.3 μM, whereas its glycosides (16, 17) had IC50 = 10.2 and 14.5 μM, respectively. A similar trend was observed for myricetin (5) and its glycosides (1820). In a kinetic study, the flavonols 35 and 1620 and the dihydroflavonols 8 and 9 behaved as competitive inhibitors, whereas other flavones (1, 2, 1315) and flavanones (6, 7) performed noncompetitive inhibition.


Limonium michelsonii Lincz. Angiotensin I converting enzyme Phenolic metabolites Competitive inhibitors 



Angiotensin I converting enzyme


Inhibitor concentration that produces a 50% decrease in activity


Inhibition constant


Maximum velocity


Michaelis–Menten constant



This work was done with research funds from the Ministry of Agriculture, Food and Rural Affairs (No. 315032-04-2-SB010) and the Next-Generation BioGreen 21 program, Rural Development Administration (SSAC, No. PJ01107001), Republic of Korea. The BK21 PLUS program supported scholarships for senior researchers and all other students.

Compliance with ethical standards

Conflict of interest

The authors declare no conflict of interest.

Supplementary material

11418_2017_1095_MOESM1_ESM.pdf (3.1 mb)
Supplementary material 1 (PDF 3176 kb)


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Copyright information

© The Japanese Society of Pharmacognosy and Springer Japan 2017

Authors and Affiliations

  • Janar Jenis
    • 1
    • 2
  • Jeong Yoon Kim
    • 1
  • Zia Uddin
    • 1
  • Yeong Hun Song
    • 1
  • Hyeong-Hwan Lee
    • 1
  • Ki Hun Park
    • 1
  1. 1.Division of Applied Life Science (BK21 Plus), IALSGyeongsang National UniversityJinjuRepublic of Korea
  2. 2.Faculty of Chemistry and Chemical TechnologyAl-Farabi Kazakh National UniversityAlmatyRepublic of Kazakhstan

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