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Journal of Natural Medicines

, Volume 71, Issue 1, pp 292–298 | Cite as

Constituents from Vitex negundo var. heterophylla and their inhibition of nitric oxide production

  • Chongyue Qiu
  • Liang Tong
  • Ting Yuan
  • Fei Wang
  • Feng Zhao
  • Lixia Chen
Note

Abstract

An iridoid glucoside, 10-p-hydroxybenzoyl-6β-hydroxyiridoid 1-O-β-d-(6′-O-p-hydroxybenzoyl)-glucopyranoside (1), and a phenol glucoside, 4-hydroxyphenethanol 3-O-β-d-(6′-O-p-hydroxybenzoyl)-glucopyranoside (2), along with nine known compounds (311) were isolated from the dried leaves of Vitex negundo var. heterophylla. Their structures were elucidated by extensive analysis of NMR spectra. All of the isolated compounds were evaluated for their inhibitory effects on nitric oxide production in RAW 264.7 macrophages. Compounds 2 and 3 exhibited obvious inhibitory effects on NO production with IC50 values of 30.76 and 49.89 μM, respectively. Molecular docking studies of compounds 2 and 3 with nitric oxide synthase (NOS) further confirmed the above results.

Keywords

Vitex negundo var. heterophylla Iridoid Phenol Nitric oxide Molecular docking 

Notes

Acknowledgments

This work was financially supported by grants from Key Projects of the National Science and Technology Pillar Program (Grant No. 2012BAI30B02) and Fund of the Educational Department of Liaoning Province (Grant No. L2011177).

Supplementary material

11418_2016_1032_MOESM1_ESM.doc (692 kb)
Supplementary material 1 (DOC 691 kb)

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Copyright information

© The Japanese Society of Pharmacognosy and Springer Japan 2016

Authors and Affiliations

  • Chongyue Qiu
    • 1
  • Liang Tong
    • 1
  • Ting Yuan
    • 1
  • Fei Wang
    • 1
  • Feng Zhao
    • 2
  • Lixia Chen
    • 1
  1. 1.Key Laboratory of Structure-Based Drug Design and Discovery, Department of Natural Products Chemistry, School of Traditional Chinese Materia Medica, Ministry of EducationShenyang Pharmaceutical UniversityShenyangChina
  2. 2.School of PharmacyYantai UniversityYantaiChina

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