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Detoxification effect of single inoculation and co-inoculation of Oudemansiella radicata and Serratia marcescens on Pb and fluoranthene co-contaminated soil

  • Soils, Sec 3 • Remediation and Management of Contaminated or Degraded Lands • Research Article
  • Published:
Journal of Soils and Sediments Aims and scope Submit manuscript

Abstract

Purpose

In this study, the pot experiments were performed to investigate that Pb and fluoranthene toxicity can be reduced and soil biological properties in co-contaminated soil can be improved by Oudemansiella radicata (O. radicata) combining with Serratia marcescens (S. marcescens).

Materials and methods

The soil used in this study was air-dried in 25 °C and sieved through a 2-mm mesh sieve, then was artificially contaminated with the different concentrations Pb. The microbial counts, the ligninolytic enzymes (Laccase and MnP), and soil enzymes (dehydrogenase and acid phosphatase) were analyzed by different methods. The Pb accumulation in mushroom and soils samples were analyzed by using the flame atomic absorption spectrometry (FAAS, SpectrAA-220Fs, United States Varian, Inc.). Fluoranthene content was analyzed using gas chromatograph-mass spectroscopy (GC–MS, Agilent 6890, USA).

Results and discussion

Results showed that Pb and fluoranthene had a negative effect on soil biological properties and mushroom. S. marcescens inoculation and O. radicata cultivation mitigated the adverse impact and improved the soil biological characteristics. S. marcescens inoculation could significantly increase the percentage of HOAc-extractable Pb and Pb concentration in O. radicata. Meanwhile, O. radicata and S. marcescens inoculation in soil could accelerate the removal of fluoranthene, and removal rate of fluoranthene was 86.39–91.95% in inoculated groups compared to 71.46–81.76% of non-inoculated groups (P < 0.05). This study demonstrated that the interaction of O. radicata and S. marcescens was effective in promoting the bioremediation of co-contaminated soil with Pb and fluoranthene, and thus making it easier for us to detoxify the pollutants.

Conclusions

This study investigated that potential of O. radicata and S. marcescen on the bioremediation of Pb-fluoranthene co-contaminated soil. The combined effect of O. radicata and S. marcescen could significantly promote the bioremediation of Pb-fluoranthene co-contaminated soil, especially for the twice inoculation of S. marcescen groups. These results demonstrated that the presence of O. radicata and S. marcescen could significantly promote the bioremediation of Pb-fluoranthene co-contaminated soil, especially for the twice inoculation of S. marcescen groups.

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Acknowledgments

The authors wish to thank Director Zhi Xing, Engineer Fulin Wang and Guiyuan Zheng from No. 605 of Sichuan Province Metallurgy Geological Bureau for their technical assistance. The authors also wish to thank Professor Guanglei Cheng and Dong Yu from Sichuan University for the technical assistance.

Funding

This work was supported by the Agricultural science and Technology Achievements Transformation Program of Sichuan Province (2017NZZJ008), the Science and Technology Supportive Project of Sichuan Province (2016NZ0050, 2017RZ0063, and 2018RZ0110), the National Science and Technology Supporting Program (2015BAD05B01-5), the NSFC (J1103518), and the Key Research and Development Program of Sichuan Province (2017SZ0188, 2017SZ0181).

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  1. The first two authors contributed equally to this work and should be considered co-first author

Authors and Affiliations

  1. Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, 610065, Sichuan, People’s Republic of China

    Lingling Li, Shanshan Guo, Yang Sun, Xuedan Li, Yufeng Gao & Heng Xu

  2. Institute of Soil and Groundwater Pollution Control of Sichuan Academy of Environmental Sciences, Chengdu, 610041, Sichuan, People’s Republic of China

    Yunzhen Li

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  1. Lingling Li
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  2. Shanshan Guo
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Correspondence to Heng Xu or Yunzhen Li.

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Li, L., Guo, S., Sun, Y. et al. Detoxification effect of single inoculation and co-inoculation of Oudemansiella radicata and Serratia marcescens on Pb and fluoranthene co-contaminated soil. J Soils Sediments 19, 3008–3017 (2019). https://doi.org/10.1007/s11368-019-02304-8

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