The role of cytokinins, ethephon, and chlorocholine chloride in the native proteolytic activity of forest soils
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Plant growth-promoting rhizobacteria (PGPRs) synthesizes and exports phytohormones which are called plant growth regulators (PGRs). These PGRs may play a regulatory role in plant growth and development. PGRs are organic substances that influence physiological processes of plants at extremely low concentrations. The objective of this study was to find out whether three PGRs, cytokinins, ethephon, and chlorocholine, have a stimulatory or inhibitory effect on the activity of native proteases in soil.
Materials and methods
A revised methodology was used to determine soil protease activity, where TRIS-HCl buffer was replaced with demineralized water. This is described as native protease activity as its activity is not affected by chemicals. The aim was to approximate the protease response to cytokinins in the native soil environment. The native soil proteolytic activity was determined spectrophotometrically.
Results and discussion
The present paper shows that cytokinin, ethephon, and chlorocholine chloride negatively affect the native proteolytic activity of forest soils with the exceptions of the organic and organomineral horizons of European beech (Fagus sylvatica L.) on a rendzic Leptosol. In addition, 6-benzylaminopurine stimulates the native proteolytic activity of the organic horizon of pedunculate oak (Quercus robur L.). A negative effect of cytokinins on the soil proteolytic activity can decrease the rate of organic matter decomposition. The results provide soil biochemists with an insight into the roles of rhizospheric substances on soil microbial activity.
This work has shown that cytokinins and PGRs inhibit the activity of native soil proteases in most of the studied forest sites. Results describe the effect of rhizospheric compounds on the activity of soil microorganisms, with potentially significant implications for the nitrogen cycle in forest soils.
KeywordsChlorocholine chloride Cytokinin Ethephone Forest soil Soil proteases
This study was created within the framework of Grant TA04020888 (Technology Agency of the Czech Republic).
- Amara U, Khalid R, Hayat R (2015) Soil bacteria and phytohormones for sustainable crop production, bacterial metabolites. In: Maheshwari DK (ed) Sustainable agroecosystem, Vol. 12 of the series sustainable development and biodiversity. Springer International Publishing, Switzerland, pp 87–103. https://doi.org/10.1007/978-3-319-24654-3_5 Google Scholar
- Anosheh HP, Emam Y, Ashraf M, Foolad MR (2012) Exogenous application of salicylic acid and chlormequat chloride alleviates negative effects of drought stress in wheat. Adv Stud Biol 4(11):501–520Google Scholar
- Cycoń M, Lewandowska A, Piotrowska-Seget Z (2012) Mineralization dynamics of chlormequat chloride (CCC) in soils of different textures. Pol J Environ Stud 21(3):595–602Google Scholar
- Farooq UZ, Bano A (2006) Effect of abscisic acid and chlorocholine chloride on nodulation and biochemical content of Vigna radiata L. under water stress. Pak J Bot 38(5):1511–1518Google Scholar
- Goswami D, Thakker JN, Dhandhukia PC (2016) Portraying mechanics of plant growth promoting rhizobacteria (PGPR): a review. Cogt Food Agric 2(1):1–19Google Scholar
- Großkinsky DK, Tafner R, Moreno MV, Stenglein SA, García de Salamone IE, Nelson LM, Novák O, Strnad M, van der Graaff E, Roitsch T (2016) Cytokinin production by Pseudomonas fluorescens G20-18 determines biocontrol activity against Pseudomonas syringae in Arabidopsis. Sci Rep 6(1):23310. https://doi.org/10.1038/srep23310 CrossRefGoogle Scholar
- Holík L, Vranová V, Rosíková J, Rejšek K (2017) Protease activity of forest soils and their responses to phenolic compounds in soil. Chem List 111:47–49Google Scholar
- IUSS Working Group WRB (2015) World Reference Base for Soil Resources 2014, update 2015 international soil classification system for naming soil and creating legends for soil maps. World Soil Resources Report No. 106. FAO, RomeGoogle Scholar
- Joint FAO (2005) Pesticide residues in food. Report of the Joint Meeting of the FAO Panel of Experts on Pesticide Residues in Food and the Environment and the WHO Core Assessment Group on Pesticide Residues, Rome, Italy, pp 20–29Google Scholar
- Khan NA, Mir MR, Nazar R, Singh S (2008) The application of ethephon (an ethylene releaser) increases growth, photosynthesis and nitrogen accumulation in mustard (Brassica juncea L.) under high nitrogen levels. Plant Biol 10(5):534–538. https://doi.org/10.1111/j.1438-8677.2008.00054.x CrossRefGoogle Scholar
- Kudoyarova GR, Melentiev AI, Martynenko EV, Timergalina LN, Arkhipova TN, Shendel GV, Kuz'mina LY, Dodd IC, Veselov SY (2014) Cytokinin producing bacteria stimulate amino acid deposition by wheat roots. Plant Physiol Biochem 83:285–291. https://doi.org/10.1016/j.plaphy.2014.08.015 CrossRefGoogle Scholar
- Laplaze L, Benkova E, Casimiro I, Maes L, Vanneste S, Swarup R, Weijers D, Calvo V, Parizot B, Begon M, Herrera-Rodriguez OR, Graham N, Doumas P, Friml J, Bogusz D, Beeckman T, Bennette M (2007) Cytokinins act directly on lateral root founder cells to inhibit root initiation. Plant Cell 19(12):3889–3900. https://doi.org/10.1105/tpc.107.055863 CrossRefGoogle Scholar
- Maheshwari DK, Dheeman S, Agarwal M (2015) Phytohormone-producing PGPR for sustainable agriculture. In: Maheshwari DK (ed) Bacterial metabolites in sustainable agroecosystem. Springer International Publishing, Switzerland, pp 159–182. https://doi.org/10.1007/978-3-319-24654-3_7 CrossRefGoogle Scholar
- Ndeddy Aka RJ, Babalola OO (2016) Effect of bacterial inoculation of strains of Pseudomonas aeruginosa, Alcaligenes feacalis and Bacillus subtilis on germination, growth and heavy metal (Cd, Cr, and Ni) uptake of Brassica juncea. Int J Phytoremediation 18(2):200–209. https://doi.org/10.1080/15226514.2015.1073671 CrossRefGoogle Scholar