Dissipation kinetics and risk assessment of iprovalicarb + propineb fungicide in tomato under different agroclimates

Abstract

Multi-location supervised field trials in India were conducted with a combination pesticide formulation (iprovalicarb 5.5% + propineb 61.25%, 66.75% WP) in tomato to study dissipation behavior at single (iprovalicarb 137.5 g a.i. ha−1 + propineb 1531.25 g a.i. ha−1) and double (iprovalicarb 275 g a.i. ha−1 + propineb 3062.5 g a.i. ha−1) dose. The samples were processed using a modified QuEChERS method for iprovalicarb and acid hydrolysis followed by carbon disulfide estimation for propineb and confirmation of their respective residues by LC–MS/MS and GC–MS. Both the fungicides in tomato fruits obey first-order kinetics irrespective of location and doses. Half-life (t1/2) values at all the four locations ranged from 1.08 to 4.67 days for iprovalicarb and 3.36 to 11.41 days for propineb in tomato. The Food Safety and Standards Authority of India (FSSAI) has set MRL of 1 mg kg−1 for propineb, but no MRL is yet fixed for iprovalicarb. Using OECD MRL calculator, the calculated MRL for iprovalicarb and propineb was found to be 2 and 4 mg kg−1, respectively. The hazard quotient (HQ) < 1, theoretical maximum daily intake (TMDI) < acceptable daily intake (ADI), TMDI < maximum permissible intake (MPI), percent acute hazard index (% aHI) ≤ 1, and percent chronic hazard index (% cHI) < 1 for both the fungicides indicated that the combination formulation will not pose any dietary risk and thus considered safe for human health.

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Data Availability

The basic data is available in the archives of the ICAR–All India Network Project on Pesticide Residues (AINP–PR), Indian Agricultural Research Institute, New Delhi, India.

References

  1. Aktar MW, Dutta P, Paramasivam M, Sengupta D (2009a) Persistence and dissipation of propineb–a dithiocarbamate fungicide in potato under East–Indian climatic conditions. Kasetsart J (Nat Sci) 43:50–55

    CAS  Google Scholar 

  2. Aktar MW, Dutta P, Paramasivam M, Sengupta D (2009b) Degradation dynamics and dissipation kinetics of a dithiocarbamate fungicide (propineb) in tomato under east–Indian climatic condition. Electronic J Environ, Agric Food Chem (EJEAFChe) 8(5):389–395

    CAS  Google Scholar 

  3. Anastassiades M, Lehotay SJ, Stajnbaher D, Schenck FJ (2003) Fast and easy multi–residue method employing acetonitrile extraction/partitioning and dispersive solid–phase extraction for the determination of pesticide residues in produce. J AOAC Int 86:412–431. https://doi.org/10.1093/jaoac/86.2.412

    CAS  Article  Google Scholar 

  4. Angioni A, Dedola F, Garau VL, Schirra M, Caboni P (2011) Fate of iprovalicarb, indoxacarb, and boscalid residues in grapes and wine by GC–ITMS analysis. J Agric Food Chem 59:6806–6812. https://doi.org/10.1021/jf2011672

    CAS  Article  Google Scholar 

  5. Banerjee H, Ganguly P, Roy S, Banerjee D, Paramasivam M, Banerjee T, Sharma KK (2010) Persistence and safety risk assessment of propineb in Indian tea. Environ Monit Assess 170:311–314. https://doi.org/10.1007/s10661-009-1234-9

    Article  Google Scholar 

  6. Bates R (2002) Pesticide residues and consumer risk assessments. Pestic Outlook 13(4):142–147. https://doi.org/10.1039/b206513b

    CAS  Article  Google Scholar 

  7. Blum M, Boehler M, Randall E, Young V, Csukai M, Kraus S, Moulin F, Scalliet G, Avrova AO, Whisson SC, Fonne-Pfister R (2010) Mandipropamid targets the cellulose synthase–like PiCesA3 to inhibit cell wall biosynthesis in the oomycete plant pathogen, Phytophthora infestans. Mol Plant Pathol 11:227–243

    CAS  Article  Google Scholar 

  8. Chadha KL (2002) Disease management in vegetables. In: Handbook of horticulture. ICAR, New Delhi, p 862

    Google Scholar 

  9. Chen F, Zhou Q, Qin C, Li Y, Zhan J (2018) Low evolutionary risk of iprovalicarb resistance in Phytophthora infestans. Pestic Biochem Physiol 152:76–83. https://doi.org/10.1016/j.pestbp.2018.09.003

    CAS  Article  Google Scholar 

  10. CIB&RC (Central Insecticide Board & Registration Committee) (2020) Registered Products: Insecticides / Pesticides Registered under section 9(3) of the Insecticides Act, 1968 for use in the Country. http://ppqs.gov.in/sites/default/files/insecticides_pesticides_registered_under_section_93_of_the_insecticides_act_1968_for_use_in_the_country_as_on_30.11.2020.pdf (Accessed 4 January, 2021)

  11. CODEX (Codex Alimentarius Commission) (2020) Codex online pesticide databases. http://www.fao.org/fao-who-codexalimentarius/codex-texts/dbs/pestres/pesticides/en/ Accessed 5 August 2020

  12. Dutzmann S (1999) Iprovalicarb (SZX 0722)–a novel fungicide with specific activity against oomycetes, PflanzenschutzNachr. Bayer (Ger Ed) 52:15–32

    CAS  Google Scholar 

  13. EFSA (European Food Safety Authority) (2015) Conclusion on the peer review of the pesticide risk assessment of the active substance iprovalicarb. EFSA J 13(4):4060. https://doi.org/10.2903/j.efsa.2015.4060

    CAS  Article  Google Scholar 

  14. EFSA (European Food Safety Authority) (2016) Conclusion on the peer review of the pesticide risk assessment of the active substance propineb. EFSA J14(11):4605. https://doi.org/10.2903/j.efsa.2016.4605

    CAS  Article  Google Scholar 

  15. EU (European Union) (2020) EU Pesticides database–European Commission. Code number 0231010. https://ec.europa.eu/food/plant/pesticides/eu-pesticides-database/public/?event=pesticide.residue.selection&language=EN Accessed 23 June 2020

  16. FAO (Food and Agriculture Organization) (2016) Submission and evaluation of pesticide residues data for the estimation of maximum residue levels in food and feed. In: FAO Plant Production and Protection Paper, Volume 225, 3rd edn. Food and Agriculture Organization of the United Nations, Rome, pp 1–283. http://www.fao.org/3/a-i5452e.pdf. Accessed 23 June 2020

  17. FAOSTAT (Food and Agriculture Organization Corporate Statistical Database) (2018) Tomato. http://www.fao.org/faostat/en/#data/QC/ Accessed 20 June 2020

  18. Feron VJ, Van Vliet PW, Notten WRF (2004) Exposure to combinations of substances: a system for assessing health risks. Environ Toxicol Pharmacol 18:215–222. https://doi.org/10.1016/j.etap.2003.11.009

    CAS  Article  Google Scholar 

  19. FSSAI (Food Safety and Standards Authority of India) (2018) Gazette Notification on Food Safety and Standards (Contaminants, Toxins and Residues) Amendment Regulation related to MRL of pesticide. The Gazette of India: Extraordinary, [Part III—Sec. 4], 24 December 2018, pp 1–51. https://fssai.gov.in/home/fss-legislation/notifications/gazette-notification.html/ Accessed 20 June 2020

  20. Gad Alla SA, Loutfy NM, Shendy AH, Ahmed MT (2015) Hazard index, a tool for a long term risk assessment of pesticide residues in some commodities, a pilot study. Regul Toxicol Pharmacol 73(3):985–991. https://doi.org/10.1016/j.yrtph.2015.09.016

    CAS  Article  Google Scholar 

  21. Herrman JL, Younes M (1999) Background to the ADI/TDI/PTWI. Regul Toxicol Pharmacol 30(2):S109–S113. https://doi.org/10.1006/rtph.1999.1335

    CAS  Article  Google Scholar 

  22. Hoskins WM (1961) Mathematical treatment of the rate of loss of pesticide residues. FAO Plant Protect Bull 9:163–168

    Google Scholar 

  23. Hu X, Li Z, Wu M, Li K, Cang T (2004) An analytical method for determination of propineb residue in tomato CNKI Journal: Agriculture: Agriculture: Acta Agriculturae Zhejiangensis. 2004:05. http://en.cnki.com.cn/Article_en/CJFDTotal-ZJNB200405009.htm Accessed 21 February 2020

  24. Islam S, Middya R, Mondal B (2018) Bioefficacy of Fungicides against Phytophthora infestans causing late blight of potato under laboratory condition. Curr J Appl Sci Technol 26(1):1–5

    Article  Google Scholar 

  25. JFCRF (Japan Food Chemical Research Foundation) (2020) Maximum residue limits (MRLs) of agricultural chemicals in foods. http://db.ffcr.or.jp/front/pesticide_detail?id=7700 Accessed 5 August 2020

  26. Jha S, Khalko S, Ashajyothi M, Bandyopadhyay S, Roy A (2017) Efficacy of combined formulations of fungicides in managing late blight disease of potato caused by Phytophthora infestans (Mont.) de Bary. Int J Curr Microbiol App Sci 6(12):765–771. https://doi.org/10.20546/ijcmas.2017.612.081

    CAS  Article  Google Scholar 

  27. Kumar YB, Shabeer TPA, Jadhav M, Banerjee K, Hingmire S, Saha S, Rai AB (2020) Analytical method validation, dissipation and safety evaluation of combination fungicides fenamidone + mancozeb and iprovalicarb + propineb in/on tomato. J Food Sci Technol 57:2061–2069. https://doi.org/10.1007/s13197-020-04240-9

    CAS  Article  Google Scholar 

  28. Lal M, Yadav S, Chand S, Kaushik SK, Singh BP, Sharma S (2015) Evaluation of fungicides against late blight (Phytophthora infestans) on susceptible and moderately resistant potato cultivars. Indian Phytopath 68(3):345–347

    Google Scholar 

  29. Lal M, Yadav S, Singh BP (2017) Efficacy of new fungicides against late blight of potato in subtropical plains of India. J Pure Appl Microbiol 11(1):599–603

    CAS  Article  Google Scholar 

  30. Lozowicka B, Kaczynski P, Patrikova AE, Kuzembekova GB, Abzhalieva AB, Sarsembayeva NB, Alihan K (2014) Pesticide residues in grain from Kazakhstan and potential health risks associated with exposure to detected pesticides. Food Chem Toxicol 64:238–248

    CAS  Article  Google Scholar 

  31. Lu XH, Zhu SS, Bi Y, Liu XL, Hao JJ (2010) Baseline sensitivity and resistance–risk assessment of Phytophthora capsici to iprovalicarb. Phytopathology 100:1162–1168

    CAS  Article  Google Scholar 

  32. Maity A, Mukherjee I (2009) Assessment of iprovalicarb, a systemic fungicide in/on cabbage (Brassica oleracea var. Capitata). Bull Environ Contam Toxicol 83(3):341–347. https://doi.org/10.1007/s00128-009-9645-4

    CAS  Article  Google Scholar 

  33. Malhat FM, El Sharkawi HM, Loufty NM, Ahmed MT (2014) Field dissipation and health hazard assessment of fenhexamid on Egyptian grape. Toxicol Environ Chem 96:722–729

    CAS  Article  Google Scholar 

  34. Mujawar S, Utture SC, Fonseca E, Matarrita J, Banerjee K (2014) Validation of a GC–MS method for the estimation of dithiocarbamate fungicide residues and safety evaluation of mancozeb in fruits and vegetables. Food Chem 150:175–181. https://doi.org/10.1016/j.foodchem.2013.10.148

    CAS  Article  Google Scholar 

  35. Mumtaz MM, Durkin PR (1992) A weight–of evidence scheme for assessing interactions in chemical mixtures. Toxicol Ind Health 8:377–406

    CAS  Article  Google Scholar 

  36. Mumtaz MM, Stpes IG, Clewell HJ, Yang RSH (1993) Risk assessment of chemical mixtures: biologic and toxicologic issues. Fundam Appl Toxicol 21:258–269

    CAS  Article  Google Scholar 

  37. NHB (National Horticulture Board) (2019) Area Production Statistics. Ministry of Agriculture & Farmers’ Welfare, Government of India. http://nhb.gov.in/Statistics.aspx?enc=WkegdyuHokljEtehnJoq0KWLU79sOQCy+W4MfOk01GFOWQSEvtp9tNHHoiv3p49g Accessed 22 June 2020

  38. NIN (National Institute of Nutrition) (2010) Nutrient requirements and recommended dietary allowance for Indians, a report of the expert group of the ICMR. ICMR–National Institute of Nutrition, Hyderabad, India. http://nutritionfoundationofindia.org/PPT-2011/Seven17-18teen/Dr-B-Sesikeran.pdf Accessed 15 August 2019

  39. NSSO (National Sample Survey Office) (2014) Household Consumption of Various Goods and Services in India 2011–12, NSS 68th Round, National Sample Survey Office, Ministry of Statistics and Programme Implementation, Government of India

  40. OECD (Organisation for Economic Co–operation and Development) (2011) OECD MRL calculator: spreadsheet for single data set and spreadsheet for multiple data set, 2 March 2011. https://www.epa.gov/pesticide-tolerances/oecd-maximum-residue-limit-calculator Accessed 15 August 2019

  41. Pant B, Bose B, Banerjee K, Koley TK, Rai AB, Singh A, Saha S (2018) Role of iprovalicarb 5.5+propineb 61.25 fungicide in the management of Blight diseases of Tomato. J Mycopathol Res 56(2):101–108

    Google Scholar 

  42. Parihar NS, Sharma BN (2011) Persistence of propineb (polymeric zinc 1, 2–propylene bisdithiocarbamate) fungicide in onion and cropped soil. Bull Environ Contam Toxicol 86:545–547. https://doi.org/10.1007/s00128-011-0256-5

    CAS  Article  Google Scholar 

  43. Patel BV, Chawla S, Gor H, Upadhyay P, Parmar KD, Patel AR, Shah PG (2016) Residue decline and risk assessment of fluopyram + tebuconazole (400SC) in/on onion (Allium cepa). Environ Sci Pollut Res 23:20871–20881

    CAS  Article  Google Scholar 

  44. Renwick AG (2002) Pesticide residue analysis and its relationship to hazard characterization (ADI/ARfD) and intake estimations (NEDI/NESTI). Pest Manag Sci 58:1073–1082

    CAS  Article  Google Scholar 

  45. Saini MK, Raza SK, Farooqi JA (2017) Dissipation kinetics study of Iprovalicarb in/on tomato fruit and grapes berries. Int J Adv Scientif Techn Res (IJASTR) 7(4):259–268

    Google Scholar 

  46. SANTE (2019) Guidance document on analytical quality control and method validation procedures for pesticide residues analysis in food and feed. Document No. SANTE/ 12682/2019

  47. Sharma ID, Patyal SK, Dubey JK, Nath A (2003) Persistence of propineb (Fungicide) in apple and tomato. Pestic Res J 15(1):60–63

    Google Scholar 

  48. Sharma KK, Shukla VR, Patel AR, Vaghela KM, Patel HK, Shah PG, Banerjee H, Banerjee T, Hudait RK, Sharma D, Sahoo SK, Singh B, Tripathy V (2016) Multilocation field trials for risk assessment of a combination fungicide fluopicolide + propamocarb in tomato. Environ Monit Assess 188(11):604. https://doi.org/10.1007/s10661-016-5610-y

    CAS  Article  Google Scholar 

  49. Sharma KK, Tripathy V, Rao CS, Bhushan VS, Reddy KN, Jyot G, Sahoo SK, Singh B, Mandal K, Banerjee H, Banerjee T, Bhattacharya S, George T, Beevi N, Sharma K, Tayade A, Gopal M, Walia S (2019) Persistence, dissipation, and risk assessment of a combination formulation of trifloxystrobin and tebuconazole fungicides in/on tomato. Regul Toxicol Pharmacol 108:104471. https://doi.org/10.1016/j.yrtph.2019.104471

    CAS  Article  Google Scholar 

  50. Sharma KK, Tripathy V, Mohapatra S, Matadha NY, Pathan ARK, Sharma BN, Dubey JK, Katna S, George T, Tayade A, Sharma K, Gupta R, Walia S (2021) Dissipation kinetics and consumer risk assessment of novaluron + lambda–cyhalothrin co–formulation in cabbage. Ecotoxicol Environ Saf 208:111494. https://doi.org/10.1016/j.ecoenv.2020.111494

    CAS  Article  Google Scholar 

  51. Tandon S, Sand NK (2009) Harvest time residues of melody duo (iprovalicarb + propineb) in soil and potato tubers. Pestology 33(2):40–45

    CAS  Google Scholar 

  52. US EPA (United States Environmental Protection Agency) (1986) Guidelines for the Health Risk Assessment of Chemical Mixtures. EPA/630/R-98/002. Risk Assessment Forum, U.S. Environmental Protection Agency, Washington, DC. September 1986 [online]. http://www.epa.gov/ncea/raf/pdfs/chem_mix/chemmix_1986.pdf. Accessed 23 July 2008

  53. US EPA (United States Environmental Protection Agency) (2020) 40 Electronic Code of Federal Regulations (e–CFR): Part 180–Tolerances and exemptions for pesticide chemical residues in food. https://www.ecfr.gov/cgi-bin/text-idx?SID=652f6661f1c740545053c400dfe56616&node=pt40.24.180&rgn=div5 Accessed 5 August 2020

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Acknowledgements

Authors are thankful to the Indian Council of Agricultural Research (ICAR), New Delhi, India (F.No. CS.8/2/2017-IA-III), for providing necessary infrastructure and facilities for conducting the study and M/s Bayer Crop Science Limited, Mumbai, India (ST No. 107), for sponsoring multi-location supervised field trials.

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Authors

Contributions

Conceptualization, funding acquisition, investigation, project administration, resources, visualization, supervision: Dr. Krishan Kumar Sharma and Dr. Vandana Tripathy

Review and editing: Dr Suresh Walia

Writing—original draft preparation: Dr. Khushbu Sharma, Dr. Rajbir Yadav, and Dr. Ruchi Gupta

Project administration, formal analysis, and investigation (KAU–Center): Dr. Thomas George

Project administration, formal analysis, and investigation (MPKV–Center): Dr. Chidanand Shiveshankar Patil and Yogesh Subhash Saindane

Project administration, formal analysis, and Investigation (IIHR–Center): Dr. Soudamini Mohapatra and Lekha Siddamallaiah

Project administration, formal analysis, and investigation (SKNAU–Center): Dr. Abdul Rashid Khan Pathan and Dr. Amit Kumar Yadav

Corresponding author

Correspondence to Krishan Kumar Sharma.

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The study does not require approval of the Institute’s Ethical Committee.

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The work has been conducted under All India Network Project on Pesticide Residues, and prior consent of all the participating authors has been obtained.

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The author vouches that the work has not been published elsewhere completely, in part, or in any other form, and that the manuscript has not been submitted to another journal.

Disclosure statement

The field trials complied with national and international guidelines and the authors have no competing financial interest. Pesticide risk assessment, food safety, and field trials are the mandate of the project, and necessary approval for the same has been accorded by the competent authority.

Conflict of interest

Krishan Kumar Sharma is a network coordinator. The rest of the authors declare no conflict of interest.

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Tripathy, V., Sharma, K.K., George, T. et al. Dissipation kinetics and risk assessment of iprovalicarb + propineb fungicide in tomato under different agroclimates. Environ Sci Pollut Res (2021). https://doi.org/10.1007/s11356-021-12919-5

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Keywords

  • Iprovalicarb
  • Propineb
  • Residue
  • Tomato
  • Consumer risk assessment
  • OECD MRL calculator