Can the sonication of polystyrene nanoparticles alter the acute toxicity and swimming behavior results for Daphnia magna?

Abstract

The seemingly ubiquitous presence of plastic debris led to a greater focus on micro- and nanoplastics research derived from the degradation process of macroplastics. The ingestion and consequent accumulation of plastics on the biota are the main concerns. Researchers strive to make assay conditions as close as possible to those of the environment. In this regard, sonication can be applied to de-agglomerate the plastic particles, but this may alter significantly their toxicity. The aim of this study was to understand the effects of the sonication process on the acute toxicity and swimming behavior of polystyrene nanoparticles using Daphnia magna as the test organism. The results show a 2-fold reduction in the acute toxicity after the sonication process; the EC50 of the PSNP-NS was 1.28 ± 0.17 mmol while for PSNP-S the EC50 was 2.77 ± 0.32 mmol, possibly through the formation of an eco-corona on the nanoplastic surface, formed from the ions dispersed in the medium or proteins secreted by the test organisms. The mean swimming distance was reduced when compared to the control group for both the PSNP-S and PSNP-NS. This is the first research stating the toxicological differences between sonicated and non-sonicated polystyrene nanoparticle samples using Daphnia magna as test organism.

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Data availability

The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.

References

  1. Albanese A, Chan WCW (2011) Effect of gold nanoparticle aggregation on cell uptake and toxicity. ACS Nano 5:5478–5489. https://doi.org/10.1021/nn2007496

    CAS  Article  Google Scholar 

  2. Besseling E, Wang B, Lürling M, Koelmans AA (2014) Nanoplastic affects growth of S. obliquus and reproduction of D. magna. Environ Sci Technol 48:12336–12343. https://doi.org/10.1021/es503001d

    CAS  Article  Google Scholar 

  3. Chu SP (1942) The influence of the mineral composition of the medium on the growth of planktonic algae: Part I. Methods and Culture Media J Ecol 30:284. https://doi.org/10.2307/2256574

    CAS  Article  Google Scholar 

  4. Cui R, Kim SW, An YJ (2017) Polystyrene nanoplastics inhibit reproduction and induce abnormal embryonic development in the freshwater crustacean Daphnia galeata. Sci Rep 7:1–10. https://doi.org/10.1038/s41598-017-12299-2

    CAS  Article  Google Scholar 

  5. Fadare OO, Wan B, Guo LH, Xin Y, Qin W, Yang Y (2019) Humic acid alleviates the toxicity of polystyrene nanoplastic particles to: Daphnia magna. Environ Sci Nano 6:1466–1477. https://doi.org/10.1039/c8en01457d

    CAS  Article  Google Scholar 

  6. Farré M, Gajda-Schrantz K, Kantiani L, Barceló D (2009) Ecotoxicity and analysis of nanomaterials in the aquatic environment. Anal Bioanal Chem 393:81–95. https://doi.org/10.1007/s00216-008-2458-1

    CAS  Article  Google Scholar 

  7. Greven AC, Merk T, Karagöz F, Mohr K, Klapper M, Jovanović B, Palić D (2016) Polycarbonate and polystyrene nanoplastic particles act as stressors to the innate immune system of fathead minnow (Pimephales promelas). Environ Toxicol Chem 35:3093–3100. https://doi.org/10.1002/etc.3501

    CAS  Article  Google Scholar 

  8. Handy RD, Cornelis G, Fernandes T, Tsyusko O, Decho A, Sabo-Attwood T, Metcalfe C, Steevens JA, Klaine SJ, Koelmans AA, Horne N (2012) Ecotoxicity test methods for engineered nanomaterials: practical experiences and recommendations from the bench. Environ Toxicol Chem 31:15–31. https://doi.org/10.1002/etc.706

    CAS  Article  Google Scholar 

  9. ISO (2012) Water quality — determination of the inhibition of the mobility of Daphnia magna Straus (Cladocera, Crustacea) — acute toxicity test. 6341:11

  10. Jiang R, Lin W, Wu J, Xiong Y, Zhu F, Bao LJ, You J, Ouyang G, Zeng EY (2018) Quantifying nanoplastic-bound chemicals accumulated in: Daphnia magna with a passive dosing method. Environ Sci Nano 5:776–781. https://doi.org/10.1039/c7en00932a

    CAS  Article  Google Scholar 

  11. Külkamp IC, Paese K, Guterres SS, Pohlmann AR (2009) Stabilization of lipoic acid by encapsulation in polymeric nanocapsules designed for cutaneous administration. Quim Nova 32:2078–2084

    Article  Google Scholar 

  12. Lehner R, Weder C, Petri-Fink A, Rothen-Rutishauser B (2019) Emergence of nanoplastic in the environment and possible impact on human health. Environ Sci Technol 53:1748–1765. https://doi.org/10.1021/acs.est.8b05512

    CAS  Article  Google Scholar 

  13. Liu L, Fokkink R, Koelmans AA (2016) Sorption of polycyclic aromatic hydrocarbons to polystyrene nanoplastic. Environ Toxicol Chem 35:1650–1655. https://doi.org/10.1002/etc.3311

    CAS  Article  Google Scholar 

  14. Liu Y, Nie Y, Wang J, Wang J, Wang X, Chen S, Zhao G, Wu L, Xu A (2018) Mechanisms involved in the impact of engineered nanomaterials on the joint toxicity with environmental pollutants. Ecotoxicol Environ Saf 162:92–102. https://doi.org/10.1016/j.ecoenv.2018.06.079

    CAS  Article  Google Scholar 

  15. Lu S, Qu R, Forcada J (2009) Preparation of magnetic polymeric composite nanoparticles by seeded emulsion polymerization. Mater Lett 63:770–772. https://doi.org/10.1016/j.matlet.2008.12.045

    CAS  Article  Google Scholar 

  16. Lynch I, Dawson KA, Lead JR, Valsami-Jones E (2014) Macromolecular coronas and their importance in nanotoxicology and nanoecotoxicology, 1st edn. Elsevier Ltd.

  17. Ma Y, Huang A, Cao S, Sun F, Wang L, Guo H, Ji R (2016) Effects of nanoplastics and microplastics on toxicity, bioaccumulation, and environmental fate of phenanthrene in fresh water. Environ Pollut 219:166–173. https://doi.org/10.1016/j.envpol.2016.10.061

    CAS  Article  Google Scholar 

  18. Murdock RC, Braydich-Stolle L, Schrand AM, Schlager JJ, Hussain SM (2008) Characterization of nanomaterial dispersion in solution prior to in vitro exposure using dynamic light scattering technique. Toxicol Sci 101:239–253. https://doi.org/10.1093/toxsci/kfm240

    CAS  Article  Google Scholar 

  19. Nasser F, Lynch I (2016) Secreted protein eco-corona mediates uptake and impacts of polystyrene nanoparticles on Daphnia magna. J Proteome 137:45–51. https://doi.org/10.1016/j.jprot.2015.09.005

    CAS  Article  Google Scholar 

  20. OECD (2004) Test No. 202: Daphnia sp. acute immobilisation test. OECD, Paris

  21. Pikuda O, Xu EG, Berk D, Tufenkji N (2018) Toxicity assessments of micro- and nanoplastics can be confounded by preservatives in commercial formulations. Environ Sci Technol Lett 6:21–25. https://doi.org/10.1021/acs.estlett.8b00614

    CAS  Article  Google Scholar 

  22. Price GJ, Smith PF (1991) Ultrasonic degradation of polymer solutions. 1. Polystyrene revisited. Polym Int 24:159–164. https://doi.org/10.1002/pi.4990240306

    CAS  Article  Google Scholar 

  23. Rist S, Baun A, Hartmann NB et al (2017) Ingestion of micro- and nanoplastics in Daphnia magna – quantification of body burdens and assessment of feeding rates and reproduction. Environ Pollut 228:398–407. https://doi.org/10.1016/j.envpol.2017.05.048

    CAS  Article  Google Scholar 

  24. Saptarshi SR, Duschl A, Lopata AL (2013) Interaction of nanoparticles with proteins: relation to bio-reactivity of the nanoparticle. J Nanobiotechnology 11:1–12. https://doi.org/10.1186/1477-3155-11-26

    CAS  Article  Google Scholar 

  25. Vicentini DS, Nogueira DJ, Melegari SP, Arl M, Köerich JS, Cruz L, Justino NM, Oscar BV, Puerari RC, da Silva MLN, Simioni C, Ouriques LC, Matias MS, Castilhos Junior AB, Matias WG (2019) Toxicological evaluation and quantification of ingested metal-core nanoplastic by Daphnia magna through fluorescence and ICP-MS methods. Environ Toxicol Chem 38:2101–2110. https://doi.org/10.1002/etc.4528

    CAS  Article  Google Scholar 

  26. Wagner M, Lambert S (2018) Freshwater microplastics. Springer International Publishing

  27. Wan J-K, Chu W-L, Kok Y-Y, Lee C-S (2018) Distribution of microplastics and nanoplastics in aquatic ecosystems and their impacts on aquatic organisms, with emphasis on microalgae. In: Reviews of Environmental Contamination and Toxicology. Springer, Cham, Kuala Lumpur, pp 133–158

  28. Zhang J, Liu D, Yang G, Han B, Wu Z (2007) Effect of ultrasound on the microstructure of polystyrene in cyclohexane: a synchrotron small-angle X-ray scattering study. Colloid Polym Sci 285:1275–1279. https://doi.org/10.1007/s00396-007-1677-x

    CAS  Article  Google Scholar 

Download references

Acknowledgments

This research was funded by the Brazilian agencies Coordination for the Improvement of Higher Education Personnel (CAPES), and the National Council for Scientific and Technological Development (CNPq) and supported by Multiuser Laboratory of Biology Studies (LAMEB).

Funding

All funding was provided from the Coordination for the Improvement of Higher Education Personnel (CAPES) and the National Council for Scientific and Technological Development (CNPq).

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All authors contributed equally to this research. VPV with aid from DJN collected all the data (characterization, acute toxicity tests, and swimming assay). DSV and WGM contributed on the results discussion by adding major considerations and contributions regarding the evaluation of the data but also the elaboration of the hypothesis.

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Correspondence to William G. Matias.

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Vaz, V.P., Nogueira, D.J., Vicentini, D.S. et al. Can the sonication of polystyrene nanoparticles alter the acute toxicity and swimming behavior results for Daphnia magna?. Environ Sci Pollut Res (2021). https://doi.org/10.1007/s11356-021-12455-2

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Keywords

  • Nanoplastics
  • Daphnia magna
  • Polystyrene nanoparticles
  • De-agglomeration method
  • Sonication
  • Sublethal effects