Active functional groups in biofilms determine the adsorption and desorption of contaminants and nutrients. Epilithic biofilms were characterized in order to understand the association between the chemistry alteration patterns and the surrounding anthropic activities of the Guaporé River watershed. The instrumental analyses included pyrolysis coupled to gas chromatography and mass spectroscopy, spectroscopy in the IR region with attenuated total reflectance, and two-dimensional nuclear magnetic resonance. Spectrometric techniques demonstrated that epilithic biofilms are mainly composed of polysaccharides, nitrogen-containing compounds, lipids, and aromatic and phenolic structures, which have functional groups characteristic of alcohols, esters, ethers, and amides. The polysaccharide levels reflect well the environmental pressures. The chemical composition of epilithic biofilms can be an effective tool for environmental assessment in watercourses, since the different anthropic actions developed in watersheds, mainly agriculture and urban areas, can modify the organic fraction of biofilms.
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All data generated during this study are included in this published article and its supplementary information files. Additional data are available from the corresponding author on reasonable request.
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The authors thank Clarissa Piccinin Frizzo for assistance with spectral measurements and Núcleo de Análises e Pesquisas Orgânicas of the Chemistry Department of the Universidade Federal de Santa Maria for technical support.
This research was funded by Mais Água of FINEP/FEPAGRO, National Council for Scientific and Technological Development (CNPq, Brazil) for fellowships awarded to DS Rheinheimer (309515/2015–7), FAPERGS (19/2551–0001641-7), and Région Nouvelle Aquitaine (France).
The authors declare that they have no competing interests.
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Fernandes, G., Bastos, M.C., Mondamert, L. et al. Organic composition of epilithic biofilms from agricultural and urban watershed in South Brazil. Environ Sci Pollut Res (2021). https://doi.org/10.1007/s11356-020-11389-5
- Anthropic activities
- Epilithic biofilms
- Chemical composition
- Functional groups
- Spectrometric techniques