Impact of sterilants on culture establishment of indigenous Musa L. varieties: a step forward for conservation


In this paper, we are focusing on study of the impact of sterilization on culture establishment of indigenous Musa variety to restore plant diversity and its importance in human life. Biodiversity supports biological system profitability where every species has a significant task to carry out. Biodiversity of plants guarantees an asset for new nourishment harvests and prescriptions. The biodiversity conservation can start from in situ and ex situ techniques. In situ strategies secure the plants in their common natural surroundings, where as ex situ techniques include safeguarding and upkeep of plant species through plant tissue culture technique. Re-establishing Indigenous plant territory is fundamental for conservation of biodiversity. Indigenous plants are those that present normally in a place where they developed. They are the biological premise whereupon the life of animals and human depends. Traditional medicines from plants are significant part of Indian culture. Banana cultivars nowadays are triploid (AAA, AAB, ABB) cross breeds of two species, Musa acuminate Colla (AA) and Musa balbisiana Colla (BB), which separately contribute the A and B genomes. The M. balbisiana which is normally present in North-Eastern belt of India has high nutrients and resistance to biotic and abiotic stresses. Nanjangud rasabale has been found in parts of Mysuru district, known for its stand-out smell, flavor, taste, and time span of sensible convenience anyway incredibly viewed as a jeopardized grouping; red bananas are phenomenal anyway concerning supplement C and potassium, they are better than yellow ones; Meitei Hei is acclaimed for its enjoyableness and incredible alluring quality. It is a cold permissive grouping thriving incredible even at 12–15 °C. The accomplishment of plant tissue culture convention relies upon explant sterilization procedure. Determination of sterilant and time span of introduction are additionally basic on the grounds that the living material should not lose their natural action and just contaminants should be wiped out during cleansing. Surface sanitization of explant is a procedure which includes the drenching of explants into suitable convergence of compound sterilants for a predetermined time bringing about the foundation of a contamination-free culture.

This is a preview of subscription content, access via your institution.

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8
Fig. 9


  1. Banaerjee N, De Langhe E (1985) A tissue culture technique for rapid clonal propagation and storage under minimal growth conditions of Musa (Banana and Plantain). Plant Cell Rep 4(6):351–354.

    Article  Google Scholar 

  2. Cheesman E.E. (1948). Classification of the bananas. Critical notes on species. Musa balbisiana Colla. Kew Bulletin,3(3), 325.

  3. FAO STAT. (2011) Food and Agriculture Organization of the United Nations

  4. Hore DK, Sharma BD, Pandey G (1992) Status of banana in North-East India. J Econ Taxon Bot 16:447–455

    Google Scholar 

  5. ICAR. (2015). Vision 2050. National research centre for Banana

    Google Scholar 

  6. Osuji JO, Okoli BE, Ortiz R (1995) Morphological characterisation of Musa cultivars. Nigerian J Genet X:8–13

  7. Joe A, Sreejith PE, Sabu M (2014) A new variety of Musa balbisianaColla (Musaceae) from South India. Phytotaxa 175(2):113–116.

    Article  Google Scholar 

  8. Buah JN (2016) In vitro growth of dwarf Cavendish Banana plantlets in different culture vessels and light intensities. Int J Agric Res 11(1):23–31.

    CAS  Article  Google Scholar 

  9. Borborah K, Borthakur SK, Tanti B (2015) Musa BalbisianaColla- taxonomy, traditional knowledge and economic potentialities of the plant in Assam, India. Indian J Tradit Knowl 15(1):116–120

    Google Scholar 

  10. Borborah K, Borthakur SK, Tanti B (2016) A new variety of Musa Balbisiana Colla from Assam. India Bangladesh J Plant Taxon 23(1):75–78.

    Article  Google Scholar 

  11. Martin KP, Pachathundikandi SK, Zhang CL, Slater A, Joseph M (2006) RAPD analysis of a variant of banana (Musa sp.) cv. Grande Naine and its propagation via shoot tip culture. In Vitro Cell Dev Biol Plant 42(2):188–192.

    CAS  Article  Google Scholar 

  12. Moore H.E. (1957). Musa and Ensete the cultivated bananas. Baileya5, 177

  13. Nayar NM (2010) The bananas: botany, origin, dispersal. Horticultural Reviews:117–164.

  14. P. Babu. (2019). An efficient protocol for in vitro regeneration of Banana var. Nanjangudurasabale(Musa spp. AAB). Int.J.Curr.Microbiol.App.Sci8(6): 3392-3402.

  15. Rajoriya P (2011) Development of protocols for micropropagation of red banana (Musa acuminata). (M. Sc. Biotechnlgy thesis) Sam Higginbottom University of agriculture, Technology and Sciences, Allahabad. 1–34

  16. Lalrinsanga R, Vanlaldiki H, Meitei WI (2013) In vitro shoot tip culture of Banana cultivar Meitei Hei. The Bioscan 8(3):839–844

    Google Scholar 

  17. Scot C. Nelson, Randy C. Ploetz, and Angela Kay Kepler. (2006). Musa species (banana and plantain). Species Profiles for Pacific Island Agroforestry

    Google Scholar 

  18. Subbaraya U (2006) Farmer's knowledge of wild Musa in India. Plant Production and Protection Division, FAO, Rome, pp 1–46

    Google Scholar 

  19. Sulistyaningsih LD, Megia R, Widjaja EA (2014) Two new records of wild bananas (Musa balbisiana and Musa itinerans) from Sulawesi. Makara J Sci 18(1):1–6.

    Article  Google Scholar 

Download references


The authors are grateful to the Amity University, Noida for facilitating us with the infrastructure and laboratory facilities for manifesting this research work.

Author information



Corresponding author

Correspondence to Susmita Shukla.

Additional information

Publisher’s note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Responsible editor: Philippe Garrigues

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Bhutani, R., Shukla, S. & Shukla, S. Impact of sterilants on culture establishment of indigenous Musa L. varieties: a step forward for conservation. Environ Sci Pollut Res 28, 3913–3919 (2021).

Download citation


  • Indigenous
  • Plant biodiversity
  • Sterilization
  • Contamination
  • Plant tissue culture