Abstract
Due to the vegetation destruction and soil desertification caused by excessive exploitation at Ganzhou ion-type rare earth mine in the mid-1980s, it is essential to carry out ecological remediation. The symbiotic mycorrhiza formed by the developed perennial ryegrass (Lolium perenne L.) roots infected with arbuscular mycorrhizal fungi (AMF) can significantly improve the growth and resistance of plants. In this study, the combination of symbiotic mycorrhiza and soil modifier was used to construct the ryegrass-AMF-soil modifier combined remediation technology, which achieved effective ecological remediation of soil tailings. The orthogonal experiment of soil modifier showed that the most efficient formula for ryegrass biomass, soil organic matter, soil alkaline hydrolysis, soil available phosphorus, and soil pH was 5 g/kg sepiolite, 3 g/kg chicken manure, 2 g/kg humic acid, and 2 g/kg biochar (A4B3C3D3), and chicken manure (B), humic acid (C), and biochar (D) had significant effects on the improvement of ryegrass biomass, soil organic matter, soil alkaline nitrogen, and soil available phosphorus. Sepiolite (A) had a significant improvement in soil pH. Furthermore, the AMF infection results indicated that Glomus moss (G.m.) had higher affinity with ryegrass. The T4 treatment-combined remediation using G.m. inoculation had the most significant effect on ryegrass growth; plant height increased by 39.19% compared with T1 treatment-inoculation using G.m. Under combined remediation, soil pH, organic matter, alkali nitrogen, and effective phosphorus content also significantly improved after combined treatment. Under G.m. inoculation treatment (T4 treatment), the soil nutrient content reached the three criteria of the soil nutrient grading standard.
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References
Anderson RC, Liberty AE (1992) Influence of supplemental inorganic nutrients on growth, survivorship, and mycorrhizal relationships of Schizachyrium scoparium (Poceae) grown in fumigated and unfumigated soil. Am J Bot 79:406–411
Bao S (2001) Soil agrochemical analysis. China Agricultural Press, Beijing
Becquer A, Garcia K, Plassard C (2018) HcPT1.2 participates in pi acquisition in Hebeloma cylindrosporum external hyphae of ectomycorrhizas under high and low phosphate conditions. Plant Signal Behav 13(10)
Chao Y, Liu W, Chen Y (2016) Structure, variation, and co-occurrence of soil microbial communities in abandoned sites of a rare earth elements mine. Environ Sci Technol 50(21):11481–11490
Chen ZP (2015) Effect of arbuscular mycorrhizal fungi on accumulation and resistance of antimony in plants. Dissertation, Hunan Agricultural University
Florian W, Helge N, Mathimaran N et al (2012) Mycorrhizal networks: common goods of plants shared under unequal terms of trade. Plant Physiol 159:2
Gao ZQ, Zhou QX (2011) Contamination from rare earth ore strip mining and its impacts on resources and eco-environment. Chin J Ecol 30(12):2915–2922 (in Chinese)
Gholamhoseini M, Ghalavand A, Dolatabadian A (2013) Effects of arbuscular mycorrhizal inoculation on growth, yield, nutrient uptake and irrigation water productivity of sunflowers grown under drought stress. Agr Water Manage 117:106–114
Guan CY, Hu A, Yu CP (2019) Stratified chemical and microbial characteristics between anode and cathode after long-term operation of plant microbial fuel cells for remediation of metal contaminated soils. Sci Total Environ 670:585–594
Hannigan RE, Sholkovitz ER (2001) The development of middle rare earth element enrichments in freshwaters: weathering of phosphate minerals. Chem Geol 175(3):495–508
Hao X, Wang D, Wang P (2015) Evaluation of water quality in surface water and shallow groundwater: a case study of a rare earth mining area in southern Jiangxi Province. China Environ Monit Assess 188(1):24
Kafle A, Garcia K, Wang X, Pfeffer PE, Strahan GD, Bucking H (2019) Nutrient demand and fungal access to resources control the carbon allocation to the symbiotic partners in tripartite interactions of Medicago truncatula. Plant Cell Environ 42:270–284
Lehmann J, Rillig MC, Thies J (2011) Biochar effects on soil biota- a review. Soil Biol Biochem 43(9):1812–1836
Li XL, George E, Marschner H (1991) Extension of the phosphorus depletion zone in VA-mycorrhizal white clover in a calcareous soil. Plant Soil 136:41–48
Li WX, Lu JW, Seneweera PSM et al (2010) Effect of fertilization on forage yield and quality, nutrients uptake and soil properties in the more intensive cropping system. J Food Agric Environ 8(2):427–434
Li FR, Liu LL, Liu JL, Yang K (2019) Abiotic and biotic controls on dynamics of labile phosphorus fractions in calcareous soils under agricultural cultivation. Sci Total Environ 681:163–174
Liu SW, Huang YY, Han ZJ (2015) Practices of the soil ecological remediation in ion-absorbed rare earth mine. J Environ Eng 4:160–165 (in Chinese)
Liu MH, Che YY, Wang LQ et al (2019) Rice straw biochar and phosphorus inputs have more positive effects on the yield and nutrient uptake of Lolium multiflorum than arbuscular mycorrhizal fungi in acidic Cd-contaminated soils. Chemosphere:235
MacLean AM, Bravo A, Harrison MJ (2017) Plant signaling and metabolic pathways enabling arbuscular mycorrhizal symbiosis. Plant Cell 29(10):2319–2335
Mishra M, Sahu RK et al (2008) Influence of organic amendments on growth, yield and quality of wheat and on soil properties during transition to organic production. Nutr Cycl Agroecosyst 82:51–60
Norini MP, Thouin H, Miard F, Battaglia-Brunet F, Gautret P, Guegan R, Le Forestier L, Morabito D, Bourgerie S, Motelica-Heino M (2019) Mobility of Pb, Zn, Ba, As and Cd toward soil pore water and plants (willow and ryegrass) from a mine soil amended with biochar. J Environ Manag 232:117–130
Pan Y, Li H (2016) Investigating heavy metal pollution in mining brownfield and its policy implications: a case study of the Bayan obo rare earth mine, Inner Mongolia. China Environ Manage 57(4):879–893
Pathan SM, Aylmore LAG, Colmer TD (2004) Turf culture under declining volume and frequency of irrigation on asandy soil amended with fly ash. Plant Soil 266:355–369
Roldan-Fajardo BE (1994) Effect of indigenous arbuscular mycorrhizal endophytes on the development of six wild plants colonizing a semi-aridarea in south-east Spain. New Phytol 127:115–121
Ruiz-lozano JM (2003) Arbuscular mycorrhizal symbiosis and alleviation of osmotic stress. New perspectives for molecular studies. Mycorrhiza 13(6):309–317
Sally K, Delphine M, Sefer B, Odile C, Moritz F (2017) Phylogenetic, structural, and functional characterization of AMT3;1, an ammonium transporter induced by mycorrhization among model grasses. Mycorrhiza 27(7):695–708
Sánz MJ, Taboada-castro MT (1998) Growth, mineral nutrition and mycorrhizal colonization of red clover and cu-cumber plants grown in a soil amended with composted urban wastes. Plant Soil 205:85–92
Sebastián M, Fernando B, Nanthi B (2012) Phytoremediation of metal-polluted soils by arbuscular mycorrhizal Fungi. Crit Rev Environ Sci Technol 42:741–775
Selvakumar G, Shagol CC, Kim K (2018) Spores associated bacteria regulates maize root K+ /Na+ ion homeostasis to promote salinity tolerance during arbuscular mycorrhizal symbiosis. BMC Plant Biol 18:109
Tian H, Drijber R, Zhang J (2013) Impact of long-term nitrogen fertilization and rotation with soybean on the diversity and phosphorus metabolism of indigenous arbuscular mycorrhizal fungi within the roots of maize (Zea mays L). Agric Ecosyst Environ 164:53–61
Toussaint JP, Starnaud M, Charest C (2004) Nlitrogen transfer and assimilation between the arbuscular mycorrhizal fungus Glomus intraradices Schenck & Smith and Ri T-DNA roots of Daucus carota L. in an invitro compartmented system. Can J Microbiol 50:251–260
Vestberg M, Saari K, Kukkonen S (2009) Mycotrophy of crops in rotation and soil amendment with peatinfluence the abundance and effectiveness of indigenous arbuscular mycorrhizal fungi in field soil. Mycorrhiza 15:447–458
Wang LH (2007) Effect of different organic fertilizers on soil properties. Dissertation, Jilin Agricultural University
Wei Z, Hao Z, Li X, Guan Z, Cai Y, Liao X (2019) The effects of phytoremediation on soil bacterial communities in an abandoned mine site of rare earth elements. Sci Total Environ 670:950–960
Whalen JK, Chang C, Clayton GW (2000) Cattle manure amendments can increase the pH of acic soils. Soil Sci Soc Am J 64(3):962–966
Xiao ZJ, Liu ZW, Zhang N (2014) Environmental impact analysis and control technology of ion rare earth mining in south of Jiangxi province. Chin Rare Earths 35(6):56–61 (in Chinese)
Ye ZH, Yang ZY, Chan YS (2001) Growth response of Sesbania rostrata and S. cannabina to sludge-amended lead /zinc mine tailings: a greenhouse study. Environ Int 26:449–455
Yi BZ, Liu XJ, Gao F (2009) Effects of different AM Fungi on growth and physiology of strawberry. Chin Sci Bull 25(6):173–176
Yu RX, Li SP, Bi YL (2014) Effect of coal mining on root growth of artemisia sphaerocephala and its self-repairing ability. Coal Sci Technol 42(2):110–113
Zhang Y (2014) An experimental research on improvement of abandoned rare earth mine tailings soil and phytoremediation. Dissertation, Jiangxi University of Science and Technology (in Chinese)
Zhong ZG, Zhou HP, Hu J, Zhang YB (2017) Research progress of green extraction technology for ion-adsorption type rare earths ore in southern China. Metal Mine 12:76–81 (in Chinese)
Acknowledgments
We also thank Prof. Zhao Zhongqiu for the guidance during the writing and modifying of the manuscript.
Funding
This study was supported by the Foundation for Public Welfare of the Ministry of Land and Resources of the People’s Republic of China (No.201411017).
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Highlights
1. Application of microorganisms in ecological remediation of rare earth tailings
2. Application of new soil amendment in ecological remediation of rare earth tailings
3. Providing a new combined remediation technology in rare earth tailings
Appendix
Appendix
Supplementary material for soil amendments used in this experiment. The screening process of the modifier was as follows.
Effect of soil amendments on soil remediation
Ryegrass was used in our study to examine soil improvement effects under different experimental treatments. Ryegrass biomass was used as an effective indicator for soil improvement. Results indicated that ryegrass biomass significantly improved under the different treatments (Fig. 7). Although the improvement effects of the four amendments were noticeable, differences between the combinations of amendments existed in regulating soil properties and increasing soil nutrient content. Although an increase in biomass improved soil nutrients, the change in soil properties was also conducive to ryegrass growth. Our results indicated that the biomass change trends of each treatment were similar. Although these trends were affected by a sudden drop in temperature, the lack of a certain nutrient level in the soil and the addition of other nutrients will not increase the effects on plant growth. Analysis of variance showed that chicken manure (B), humic acid (C), and biochar (D) had significant effects on ryegrass biomass while sepiolite (A) had no significant effect. The effect these amendments had on biomass differed, having the order of influence of: chicken manure(B) > humic acid (C) > biochar (D) > sepiolite (A). Results for sepiolite indicated that treatment A4 had the largest value (1.249). There were significant differences between A4 and A1, A2, and A3, but no significant differences among A1, A2, and A3. For chicken manure, B2 recorded the largest mean value (1.225); no significant differences existed between B2 and B1 or between B3and B4. Similarly, C3 was the largest for humic acid (1.058); significant differences were present between C3, C1, and C4. No significant difference was present between C1 and C4. Results for biochar recorded treatment D3 to be the largest (1.139). Significant differences existed between D3 and D2, D4, D1, and D4; however, no significant difference was found between D2 and D4. The effects of the four amendments on ryegrass biomass were comprehensively analyzed and the optimum level combination was A4B2C3D3.
Effects of soil amendments on soil physical and chemical properties
Effect of soil ameliorants on soil pH
pH is the most basic index to measure soil acidity and alkalinity. Different treatments in our experiments had a significant influence on soil pH (Fig. 8). After soil amelioration, there were obvious differences among the different treatments. The trend of change for soil pH was from low to high. Soil pH for each treatment was higher than the control, with differences being significant. Variance analysis indicated that the order of the four factors affecting soil pH was sepiolite (A) > chicken manure (B) > humic acid (C) > biochar (D). Our results indicate that as the concentration of sepiolite increased, soil pH increased, thereby making this an important factor influencing soil pH.
Variance analysis results showed that sepiolite (A) had a significant effect on soil improvement in the mining area, while chicken manure (B), humic acid (C), and biochar (D) had no significant effect (although the degree of effect differed). For sepiolite, treatment A4 recorded the highest value (6.820), having a significant difference with A1 and A2. A1 and A3 had a significant difference and A1, A2, and A3 had no significant difference. B2 recorded the largest mean value for chicken manure (6.535). Significant differences were recorded between B2 and B1; B4, B2, and B3; and B1 and B4. C4 recorded the largest mean value for humic acid (6.442). Significant differences were recorded between C1 and C2 and between C3 and C4, but there were no significant differences between C2, C3, and C4. The largest mean value for biochar was recorded in treatment D3 (6.370). There were significant differences between D1 and D3 and between D2 and D4. No significant differences were recorded between D1 and D3 or between D2 and D4. The optimum combination of amendments to improve soil pH was: A4B2C4D3.
Effects of soil ameliorants on soil organic matter content
Soil nutrients have a direct effect on crop yield and quality, and soil organic matter content is an effective indicator of soil fertility. Soil organic matter affects the physical properties of soil, such as fertilizer effects, water content, pore size, and buffer; organic matter is also a carbon source and an energy source for soil microorganisms.
Soil organic matter content of the improved soil was higher than that of the control, with a trend of low to high (Fig. 9). Variance analysis results showed that chicken manure (B), humic acid (C), and biochar (D) had significant effects on the improvement of soil organic matter in mining area, while sepiolite (A) had no significant effect. The degree of influence between the amendments differed. As chicken manure (B), humic acid (C), and biochar (D) are rich in organic matter, these amendments had obvious effects on soil improvement. The effect of the four amendment treatments improved soil organic matter in the order of C > D > B > A.
Variance analysis showed that treatment A4 recorded the highest value for sepiolite (2.015), and that significant differences between A4 and A1 and between A2 and A3 existed. There were no significant differences among A1, A2, and A3. The greatest value for chicken manure was recorded in treatment B3(2.135), and significant differences were recorded between B3 and B1, B4, but no significant difference between B1 and B4. Results for humic acid recorded treatment C3 to have the greatest value (3.127), and the difference between C3 and C1, C2, and C3 was significant; there were no significant differences between C1, C2, and C3. D3 recorded the highest value for biochar amendment (2.197), and D3 and D4 were significantly different from D1 and D2; no significant difference was recorded between D3 and D4 or between D1 and D2. The optimum level combination of the four amendment treatments for soil organic matter was A4B3C3D3.
Effect of soil amendment on soil alkaline nitrogen content
The main source of nitrogen in the soil was from soil alkaline nitrogen. Nitrogen from this source is easily absorbed by plants, and it is mainly in the form of ammonium nitrogen, nitrate nitrogen, amino acids, amides, and easily decomposed proteins. Alkaline nitrogen plays an important role in the growth of ryegrass, and it can have a sensitive reaction to soil nitrogen dynamics and nitrogen supply during ryegrass growth periods. The content of alkaline nitrogen in the soil is related to biomass and nitrogen uptake of the ryegrass. Results for variance analysis showed that chicken manure (B), humic acid (C), and biochar (D) had significant effects on the improvement of alkaline nitrogen in the mining area; the effect of sepiolite (A) was not significant. Our results indicate that the application of a soil amendment can significantly improve alkaline nitrogen in the soil (Fig. 10). We believe that the addition of soil amendments can increase soil pH, and an increase in soil enzyme activity promotes the activation of soil nitrogen.
Analysis of variance showed that the four amendment treatments affected soil alkaline nitrogen content in the order of D > B > A > C. Results for sepiolite recorded treatment A3 to have the highest value (44.625), and significant differences existed between A3 and A1 and A4 and between A2 and A4. There was no significant difference between A1 and A4. Chicken manure results indicated that treatment B2 had the highest value (43.750), and significant differences existed between B2 and B1, B3, and B4; no significant difference was recorded between B1, B3, and B4. C3 recorded the highest value for humic acid (37.395); there were no significant differences between C3 and C1 or between C2 and C4. Treatment D2 recorded the highest value for biochar (45.500), and D2 had significant differences with D1 and D3. There was no significant difference between D1 and D3. Our results indicate that the optimal combination of the effects of the soil amendments on soil alkaline nitrogen content was A3B2C3D2.
Effect of soil amendment on soil available phosphorus content
As phosphorus is fixed in the soil when it is in a null state, the effective phosphorus content of a soil can therefore be used as an important indicator for evaluating soil fertility. Phosphorus is also an important nutrient for the growth of ryegrass. Our results indicate that after the addition of the soil amendments to the mine soil, differences between treatments was significant. Soil-available phosphorus content was higher than the control, and the trend was from low to high (Fig. 11). Compared with other studies, the combination of bio-black carbon and inorganic fertilizer into red soil improved the physical and chemical properties and nutrient content of red soil. Results for analysis of variance showed that chicken manure (B), humic acid (C), and biochar (D) have significant effects on the improvement of available phosphorus in the mining area; the effect of sepiolite (A) was not significant. The order of improvement of soil-available phosphorus using the four amendments was A > B > D > C.
Analysis of variance results indicated that treatment A4 had the highest value for sepiolite (37.710), and that there was a significant difference between A4 and A1 and A3 (P < 0.05). A significant difference also existed between A1 and A2. Chicken manure results showed that treatment B3 had the highest value (32.111), and that the difference between B3and B1, B4 was significant. Differences between B2 and B3 and B1 and B4 were not significant. Treatment C3 recorded the highest values for humic acid (29.831), and no significant difference was recorded between C1, C2, and C3; the difference between C3 and other values was significant. Biochar treatments recorded D3 to have the highest value (30.363). D3 and D4 recorded significant differences with D1 and D2, and there was no significant difference between D3 and D4. Comprehensive analysis of the improvement of soil-available phosphorus using the four amendments indicated that the optimal combination was A4B3C3D3.
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Yang, Q., Zhao, Z., Hou, H. et al. The effect of combined ecological remediation (plant microorganism modifier) on rare earth mine wasteland. Environ Sci Pollut Res 27, 13679–13691 (2020). https://doi.org/10.1007/s11356-020-07886-2
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DOI: https://doi.org/10.1007/s11356-020-07886-2