Environmental Science and Pollution Research

, Volume 26, Issue 4, pp 3350–3356 | Cite as

Cytotoxic and genotoxic effect of oxyfluorfen on hemocytes of Biomphalaria glabrata

  • Maíra de Vasconcelos Lima
  • Williams Nascimento de SiqueiraEmail author
  • Hianna Arely Milca Fagundes Silva
  • José de Melo Lima Filho
  • Elvis Joacir de França
  • Ana Maria Mendonça de Albuquerque Melo
Research Article


Chemicals released from anthropogenic activities such as industry and agriculture often end up in aquatic ecosystems. These substances can cause serious damage to these ecosystems, thus threatening the conservation of biodiversity. Among these substances are pesticides, such as oxyfluorfen, a herbicide used for the control of grasses and weeds. Considering its widespread use, it is important to investigate the possible toxicity of this compound to aquatic organisms, especially invertebrates. Hence, the use of biological systems able to detect such effects is of great importance. The mollusk Biomphalaria glabrata has been shown to be useful as an environmental indicator to assess the potential ecological effects of physical and chemical stressors in freshwater environments. The present study sought to detect mutagenic changes in hemocytes of B. glabrata exposed to oxyfluorfen. To perform these tests, this study used ten animals per group, exposed acutely (48 h) and chronically (15 days) to oxyfluorfen. The herbicide concentrations were 0.125, 0.25, and 0.5 mg/L. The results showed that oxyfluorfen induced significant frequencies of micronuclei, binucleated cells, and apoptosis in hemocytes of mollusks when compared to the control group. Unlike chronic exposure, acute exposure was dose-dependent. The present study’s results demonstrate the cytotoxic and genotoxic effects of oxyfluorfen on hemocytes of B. glabrata.


Biomphalaria glabrata Genotoxic Hemocyte Herbicide Mollusk Oxyfluorfen 


Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Maíra de Vasconcelos Lima
    • 1
    • 2
    • 3
  • Williams Nascimento de Siqueira
    • 1
    • 2
    • 3
    Email author
  • Hianna Arely Milca Fagundes Silva
    • 1
    • 3
  • José de Melo Lima Filho
    • 4
  • Elvis Joacir de França
    • 2
  • Ana Maria Mendonça de Albuquerque Melo
    • 1
    • 3
  1. 1.Departamento de Biofísica e RadiobiologiaUniversidade Federal de PernambucoRecifeBrazil
  2. 2.Serviço de Monitoração AmbientalCentro Regional de Ciências Nucleares do NordesteRecifeBrazil
  3. 3.Laboratório de RadiobiologiaUniversidade Federal de Pernambuco (UFPE)RecifeBrazil
  4. 4.Grupo de Dosimetria NuméricaInstituto Federal de Educação Ciência e Tecnologia de PernambucoRecifeBrazil

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