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Metal release and sequestration from black slate mediated by a laccase of Schizophyllum commune

  • New Toxic Emerging Contaminants: Beyond the Toxicological effects
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Abstract

Schizophyllum commune is a filamentous basidiomycete which can degrade complex organic macromolecules like lignin by the secretion of a large repertoire of enzymes. One of these white rot enzymes, laccase, exhibits a broad substrate specificity and is able to oxidize a variety of substances including carbonaceous rocks. To investigate the role of laccase in bioweathering, laccase gene lcc2 was overexpressed, and the influence on weathering of black slate, originating from a former alum mine in Schmiedefeld, Germany, was examined. The metal release from the rock material was enhanced, associated with a partial metal accumulation into the mycelium. A sequestration of metals could be shown with fluorescent staining methods, and an accumulation of Zn, Cd, and Pb was visualized in different cell organelles. Additionally, we could show an increased metal resistance of the laccase overexpressing strain.

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Acknowledgements

This project was supported by the Deutsche Forschungsgesellschaft through GRK 1257 and JSMC.

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Authors and Affiliations

  1. Institute of Microbiology, Microbial Communication, Friedrich Schiller University, Jena, Germany

    Julia Kirtzel, Eric Leon Scherwietes, Katrin Krause & Erika Kothe

  2. Institute of Geosciences, Applied Geology, Friedrich Schiller University, Jena, Germany

    Dirk Merten

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  1. Julia Kirtzel
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  2. Eric Leon Scherwietes
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  3. Dirk Merten
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  4. Katrin Krause
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  5. Erika Kothe
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Correspondence to Erika Kothe.

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Kirtzel, J., Scherwietes, E.L., Merten, D. et al. Metal release and sequestration from black slate mediated by a laccase of Schizophyllum commune. Environ Sci Pollut Res 26, 5–13 (2019). https://doi.org/10.1007/s11356-018-2568-z

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