Coordinated effects of lead toxicity and nutrient deprivation on growth, oxidative status, and elemental composition of primed and non-primed rice seedlings

  • Fahad Khan
  • Saddam Hussain
  • Mohsin Tanveer
  • Sehrish Khan
  • Hafiz Athar Hussain
  • Biland Iqbal
  • Mingjian Geng
Research Article

Abstract

Rice crop is highly susceptible to the toxic levels of lead (Pb) during early growth stages. Moreover, a sufficient availability of mineral nutrients is critical for survival of plants particularly under stressful conditions. An experiment was carried out to unravel the coordinated effects of Pb stress (1-mM PbCl2) and different nutrient treatments (sufficient nutrient supply, nitrogen (N) deprivation, phosphorus (P) deprivation, and potassium (K) deprivation) on morphological growth, reactive oxygen species (ROS), antioxidants, and nutrient status in primed and non-primed rice seedlings. Seeding were primed with distilled water, 60-μM selenium, or 100-mg L−1 salicylic acid. Results indicated that Pb toxicity did not affect the root growth, but severely reduced the shoot growth (length and biomass) of rice in N- or P-deprived seedlings. Rice seedlings grown with sufficient supply of nutrients or K-deprivation showed no growth reduction under Pb toxicity. Exposure of Pb stress triggered the production of ROS (H2O2, O2˙, OH) and lipid peroxidation rate particularly under N- or P-deprivation. Moreover, the shoot accumulations of macronutrients (P in particular) were also restricted under Pb toxicity. Seed priming treatments effectively alleviated the undesirable effects of Pb stress on rice growth. The primed rice seedlings showed minimal oxidative damage caused by excessive generation of ROS under Pb stress and/or nutrient deprivation. Seed priming strengthened the antioxidative defense system of rice seedlings by regulating the activities/levels of superoxide dismutase, catalase, peroxidase, and glutathione in rice leaves. Moreover, better accumulation of essential nutrients in primed rice seedlings prevented the excess uptake and translocation of Pb, as evident by the lowered shoot accumulation of Pb.

Keywords

Antioxidants Lead Nutrient deprivation Reactive oxygen species Rice seedlings Seed priming 

Notes

Funding information

We acknowledge the Special Fund for Agro-Scientific Research in the Public Interest of China (Project Nos. 201103005 and 201503122).

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Fahad Khan
    • 1
  • Saddam Hussain
    • 1
    • 2
  • Mohsin Tanveer
    • 3
  • Sehrish Khan
    • 4
  • Hafiz Athar Hussain
    • 5
  • Biland Iqbal
    • 6
  • Mingjian Geng
    • 1
  1. 1.College of Resources and EnvironmentHuazhong Agricultural UniversityWuhanChina
  2. 2.Department of AgronomyUniversity of AgricultureFaisalabadPakistan
  3. 3.Tasmania Institute of AgricultureUniversity of TasmaniaHobartAustralia
  4. 4.Department of Environmental SciencesUniversity of PeshawarKhyber PakhtunkhwaPakistan
  5. 5.College of Agronomy and BiotechnologySouthwest UniversityChongqingChina
  6. 6.Department of Agriculture ResearchKhyber PakhtunkhwaPakistan

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