Valorisation of tuna processing waste biomass: isolation, purification and characterisation of four novel antioxidant peptides from tuna by-product hydrolysate

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Abstract

Tuna protein hydrolysate (TPH) was prepared by hydrolysis with Prolyve BS and fractionated by membranes process. The antioxidant activities of recovered peptide fractions were evaluated. Four novel antioxidant peptides that were isolated from nanofiltration retentate exhibited the highest antioxidant activity, using gel chromatography and reversed phase high-performance liquid chromatography. The amino acid sequences of isolated peptides were identified as Tyr-Glu-Asn-Gly-Gly (P2), Glu-Gly-Tyr-Pro-Trp-Asn (P4), Tyr-Ile-Val-Tyr-Pro-Gly (P7) and Trp-Gly-Asp-Ala-Gly-Gly-Tyr-Tyr (P8) with molecular weights of 538.46, 764.75, 710.78 and 887.85 Da, respectively. P2, P4, P7 and P8 exhibited good scavenging activities on hydroxyl radical (IC50 0.41, 0.327, 0.17 and 0.042 mg/ml), DPPH radical (IC50 0.666, 0.326, 0.451 and 0.377 mg/ml) and superoxide radical (IC50 0.536, 0.307, 0.357 and 0.115 mg/ml). P7 was effective against lipid peroxidation in the model system. The isolated peptides might be useful used as natural food additive in food industry and formulation of nutritional products.

Keywords

Valorisation Tuna waste biomass hydrolysate Antioxidant activity Membrane fractionation Peptide Amino acid composition Amino acid sequence 

References

  1. Ahn C-B, Je J-Y, Cho Y-S (2012) Antioxidant and anti-inflammatory peptide fraction from salmon byproduct protein hydrolysates by peptic hydrolysis. Food Res Int 49:92–98CrossRefGoogle Scholar
  2. Ahn C-B, Kim J-G, Je J-Y (2014) Purification and antioxidant properties of octapeptide from salmon byproduct protein hydrolysate by gastrointestinal digestion. Food Chem 147:78–83CrossRefGoogle Scholar
  3. AOAC (2005) Official methods of analysis, 16th edn. Association of Official Analytical Chemists, Washington DCGoogle Scholar
  4. Bersuder P, Hole M, Smith G (1998) Antioxidants from a heated histidine-glucose model system. I: investigation of the antioxidant role of histidine and isolation of antioxidants by high-performance liquid chromatography. J Am Oil Chem Soc 75:181–187CrossRefGoogle Scholar
  5. Chen H-M, Muramoto K, Yamauchi F (1995) Structural analysis of Antioxidative peptides from Soybean .beta.-Conglycini. J Agric Food Chem 43:574–578CrossRefGoogle Scholar
  6. Chi C-F, Wang B, Deng Y-Y, Wang Y-M, Deng S-G, Ma J-Y (2014) Isolation and characterization of three antioxidant pentapeptides from protein hydrolysate of monkfish (Lophius litulon) muscle. Food Res Int 55:222–228CrossRefGoogle Scholar
  7. Chung SK, Osawa T, Kawakishi S (1997) Hydroxyl radical scavenging effects of spices and scavengers from brown mustard (Brassica nigra). Biosci Biotechnol Biochem 61:118–123CrossRefGoogle Scholar
  8. Diaz MN, Frei B, Vita JA, Keaney JF (1997) Antioxidants and atherosclerotic heart disease. N Engl J Med 337:408–416CrossRefGoogle Scholar
  9. FAO (2008) Fisheries departement: La situation mondiale des pêches et de l’aquaculture. FAO, RomeGoogle Scholar
  10. Girgih AT, Udenigwe CC, Hasan FM, Gill TA, Aluko RE (2013) Antioxidant properties of Salmon (Salmo salar) protein hydrolysate and peptide fractions isolated by reverse-phase HPLC. Food Res Int 52:315–322CrossRefGoogle Scholar
  11. Guo H, Kouzuma Y, Yonekura M (2009) Structures and properties of antioxidative peptides derived from royal jelly protein. Food Chem 113:238–245CrossRefGoogle Scholar
  12. Hsu K-C (2010) Purification of antioxidative peptides prepared from enzymatic hydrolysates of tuna dark muscle by-product. Food Chem 122:42–48CrossRefGoogle Scholar
  13. Je J-Y, Qian Z-J, Byun H-G, Kim S-K (2007) Purification and characterization of an antioxidant peptide obtained from tuna backbone protein by enzymatic hydrolysis. Process Biochem 42:840–846CrossRefGoogle Scholar
  14. Je JY, Qian ZJ, Lee SH, Byun HG, Kim SK (2008) Purification and antioxidant properties of bigeye tuna (Thunnus obesus) dark muscle peptide on free radical-mediated oxidative systems. J Med Food 11(4):629–637CrossRefGoogle Scholar
  15. Je J-Y, Cha J-Y, Cho Y-S, Ahn H-Y, Lee JH, Cho Y-S, Ahn C-B (2013) Hepatoprotective effect of peptic hydrolysate from salmon pectoral fin protein byproducts on ethanol-induced oxidative stress in Sprague Dawley rats. Food Res Int 51:648–653CrossRefGoogle Scholar
  16. Kim SK, Mendis E (2006) Bioactive compounds from marine processing byproducts—a review. Food Res Int 39:383–393CrossRefGoogle Scholar
  17. Klompong V, Benjakul S, Kantachote D, Shahidi F (2007) Antioxidative activity and functional properties of protein hydrolysate of yellow stripe trevally (Selaroides leptolepis) as influenced by the degree of hydrolysis and enzyme type. Food Chem 102:1317–1327CrossRefGoogle Scholar
  18. Kristinsson HG, Rasco BA (2000) Fish protein hydrolysates: production, biochemical, and functional properties. Crit Rev Food Sci Nutr 40:43–81CrossRefGoogle Scholar
  19. Li R, Yu H, Xing R, Liu S, Qing Y, Li K, Li B, Meng X, Cui J, Li P (2012) Isolation, identification and characterization of a novel antioxidant protein from the nematocyst of the jellyfish Stomolophus meleagris. Int J Biol Macromol 51:274–278CrossRefGoogle Scholar
  20. Li Z, Wang B, Chi C, Gong Y, Luo H, Ding G (2013a) Influence of average molecular weight on antioxidant and functional properties of cartilage collagen hydrolysates from Sphyrna lewini, Dasyatis akjei and Raja porosa. Food Res Int 51:283–293CrossRefGoogle Scholar
  21. Li Z, Wang B, Chi C, Gong Y, Tang J, Luo H (2013b) Purification and characterization of an antioxidant glycoprotein from the hydrolysate of Mustelus griseus. Int J Biol Macromol 52:267–274CrossRefGoogle Scholar
  22. Liu F, Ooi VEC, Chang ST (1997) Free radical scavenging activities of mushroom polysaccharide extracts. Life Sci 60:763–771CrossRefGoogle Scholar
  23. Luo H-Y, Wang B, Li Z-R, Chi C-F, Zhang Q-H, He G-Y (2013) Preparation and evaluation of antioxidant peptide from papain hydrolysate of Sphyrna lewini muscle protein. LWT Food Sci Technol 51:281–288CrossRefGoogle Scholar
  24. Memarpoor-Yazdi M, Mahaki H, Zare-Zardini H (2013) Antioxidant activity of protein hydrolysates and purified peptides from Zizyphus jujuba fruits. J Funct Foods 5:62–70CrossRefGoogle Scholar
  25. Mendis E, Rajapakse N, Byun H-G, Kim S-K (2005) Investigation of jumbo squid (Dosidicus gigas) skin gelatin peptides for their in vitro antioxidant effects. Life Sci 77:2166–2178CrossRefGoogle Scholar
  26. Mitsuda H, Yasumoto K, Iwami K (1996) Antioxidative action of indole compounds during the autoxidation of linoleic acid. Eiyo Shokuryo 19:210–214CrossRefGoogle Scholar
  27. Najafian L, Babji AS (2015) Isolation, purification and identification of three novel antioxidative peptides from patin (Pangasius sutchi) myofibrillar protein hydrolysates. LWT Food Sci Technol 60:452–461CrossRefGoogle Scholar
  28. Nazeer RA, Sampath Kumar NS, Jai Ganesh R (2012) In vitro and in vivo studies on the antioxidant activity of fish peptide isolated from the croaker (Otolithes ruber) muscle protein hydrolysate. Peptides 35:261–268CrossRefGoogle Scholar
  29. Ngo D-H, Ryu B, Vo T-S, Himaya SWA, Wijesekara I, Kim S-K (2011) Free radical scavenging and angiotensin-I converting enzyme inhibitory peptides from Pacific cod (Gadus macrocephalus) skin gelatine. Int J Biol Macromol 49:1110–1116CrossRefGoogle Scholar
  30. Opheim M, Slizyte R, Sterten H, Provan F, Larssen E, Kjos NP (2015) Hydrolysis of Atlantic salmon (Salmo salar) rest raw materials—effect of raw material and processing on composition, nutritional value, and potential bioactive peptides in the hydrolysates. Process Biochem 50:1247–1257CrossRefGoogle Scholar
  31. Osawa T, Namiki M (1985) Natural antioxidants isolated from Eucalyptus leaf waxes. J Agric Food Chem 33:777–780CrossRefGoogle Scholar
  32. Peng X, Xiong YL, Kong B (2009) Antioxidant activity of peptide fractions from whey protein hydrolysates as measured by electron spin resonance. Food Chem 113:196–201CrossRefGoogle Scholar
  33. Pownall TL, Udenigwe CC, Aluko RE (2010) Amino acid composition and antioxidant properties of pea seed (Pisum sativum L.) enzymatic protein hydrolysate fractions. J Agric Food Chem 58(8):4712–4718CrossRefGoogle Scholar
  34. Qian Z-J, Jung W-K, Byun H-G, Kim S-K (2008) Protective effect of an antioxidative peptide purified from gastrointestinal digests of oyster, Crassostrea gigas against free radical induced DNA damage. Bioresour Technol 99:3365–3371CrossRefGoogle Scholar
  35. Rajapakse N, Mendis E, Byun H-G, Kim S-K (2005) Purification and in vitro antioxidative effects of giant squid muscle peptides on free radical-mediated oxidative systems. J Nutr Biochem 16:562–569CrossRefGoogle Scholar
  36. Ranathunga S, Rajapakse N, Kim S-K (2006) Purification and characterization of antioxidative peptide derived from muscle of conger eel (Conger myriaster). Eur Food Res Technol 222:310–315CrossRefGoogle Scholar
  37. Sabeena Farvin KH, LLk A, Nielsen HH, Jacobsen C, Jakobsen G, Johansson I, Jessen F (2014) Antioxidant activity of Cod (Gadus morhua) protein hydrolysates: in vitro assays and evaluation in 5% fish oil-in-water emulsion. Food Chem 149:326–334CrossRefGoogle Scholar
  38. Saidi S, Ben Amar R (2016) Valorisation of tuna processing waste biomass for recovery of functional and antioxidant peptides using enzymatic hydrolysis and membrane fractionation process. Environ Sci Pollut Res 23:21070–21085CrossRefGoogle Scholar
  39. Saidi S, Deratani A, Belleville M-P, Amar RB (2014) Production and fractionation of tuna by-product protein hydrolysate by ultrafiltration and nanofiltration: Impact on interesting peptides fractions and nutritional properties. Food Res Int 65(Part C):453–461CrossRefGoogle Scholar
  40. Samaranayaka AGP, Li-Chan ECY (2011) Food-derived peptidic antioxidants: a review of their production, assessment, and potential applications. J Funct Foods 3:229–254CrossRefGoogle Scholar
  41. Sampath Kumar NS, Nazeer RA, Jaiganesh R (2011) Purification and biochemical characterization of antioxidant peptide from horse mackerel (Magalaspis cordyla) viscera protein. Peptides 32:1496–1501CrossRefGoogle Scholar
  42. Sampath Kumar NS, Nazeer RA, Jaiganesh R (2012) Purification and identification of antioxidant peptides from the skin protein hydrolysate of two marine fishes, horse mackerel (Magalaspis cordyla) and croaker (Otolithes ruber). Amino Acids 42:1641–1649CrossRefGoogle Scholar
  43. Sarmadi BH, Ismail A (2010) Antioxidative peptides from food proteins: a review. Peptides 31:1949–1956CrossRefGoogle Scholar
  44. Shahidi F, Han X-Q, Synowiecki J (1995) Production and characteristics of protein hydrolysates from capelin (Mallotus villosus). Food Chem 53:285–293CrossRefGoogle Scholar
  45. Song R, Wei R-B, Ruan G-Q, Luo H-Y (2015) Isolation and identification of antioxidative peptides from peptic hydrolysates of half-fin anchovy (Setipinna taty). LWT Food Sci Technol 60:221–229CrossRefGoogle Scholar
  46. Song R, K-q Z, R-b W (2016) In vitro antioxidative activities of squid (Ommastrephes bartrami) viscera autolysates and identification of active peptides. Process Biochem 51:1674–1682CrossRefGoogle Scholar
  47. Udenigwe CC, Aluko RE (2011) Chemometric analysis of the amino acid requirements of antioxidant food protein hydrolysates. Int J Mol Sci 12:3148–3161CrossRefGoogle Scholar
  48. Wang B, Li Z-R, Chi C-F, Zhang Q-H, Luo H-Y (2012) Preparation and evaluation of antioxidant peptides from ethanol-soluble proteins hydrolysate of Sphyrna lewini muscle. Peptides 36:240–250CrossRefGoogle Scholar
  49. Wang B, Li L, Chi C-F, Ma J-H, Luo H-Y, Xu Y-F (2013) Purification and characterisation of a novel antioxidant peptide derived from blue mussel (Mytilus edulis) protein hydrolysate. Food Chem 138:1713–1719CrossRefGoogle Scholar
  50. Xie Z, Huang J, Xu X, Jin Z (2008) Antioxidant activity of peptides isolated from alfalfa leaf protein hydrolysate. Food Chem 111:370–376CrossRefGoogle Scholar
  51. Zhang Y, Duan X, Zhuang Y (2012) Purification and characterization of novel antioxidant peptides from enzymatic hydrolysates of tilapia (Oreochromis niloticus) skin gelatine. Peptides 38:13–21CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Laboratory of Materials Science and Environment, Faculty of Science of SfaxUniversity of SfaxSfaxTunisia
  2. 2.Animal Ecophysiology Laboratory, Faculty of Sciences of SfaxUniversity of SfaxSfaxTunisia

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