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Environmental Science and Pollution Research

, Volume 25, Issue 16, pp 15609–15615 | Cite as

Ameliorative effects of nano-elemental selenium against hexavalent chromium-induced apoptosis in broiler liver

  • Liu Xueting
  • Mujeeb Ur Rehman
  • Khalid Mehmood
  • Shucheng Huang
  • Xinxin Tian
  • Xiaoxing Wu
  • Donghai Zhou
Research Article

Abstract

The current study examined the ameliorative effects of nano-elemental selenium (Nano-Se) against chromium-VI (K2Cr2O7)-induced apoptosis in chickens. The expression of apoptosis-related genes was evaluated by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and western blot. A total of 60, one-day-old broiler chickens allotted to six equal groups, i.e., control group (standard diet), Cr(VI)-exposed group (K2Cr2O7 via drinking water), Nano-Se group (Nano-Se at 0.5 mg/kg via diet), protection group (K2Cr2O7 + Nano-Se), cure group (K2Cr2O7 for initial 2 weeks and then Nano-Se), and prevention group (opposite to the cure group) and were detected by the activities of pro-apoptosis (Bax, Caspase-3) and anti-apoptosis (Bcl-2) genes expression at day 35 of the experiment. Intense apoptosis was observed in liver tissues of chickens exposed to K2Cr2O7. The Nano-Se supplementation caused a significant decrease (P < 0.01) in the mRNA expression levels of Bax and Caspase-3 genes, while significantly elevated (P < 0.05) mRNA expression level of Bcl-2 gene was observed in Nano-Se experimental groups as compare to control and Cr(VI)-exposed group. The results quantified by the RT-qPCR were further confirmed by the western blot analysis. Altogether, these results suggest anti-apoptotic effects of Nano-Se in the chicken liver, which is interesting for further study. The present findings suggested that Nano-Se has protective effects against K2Cr2O7-induced apoptosis in broilers liver and can serve a key role as a protective agent against apoptosis.

Keywords

Apoptosis Chickens Chromium-VI (K2Cr2O7Nano-selenium 

Notes

Authors’ contributions

LX, DZ, and MR participated in the conception and design of the study and wrote the manuscript. LX, MR, KM, SCH, XT, and XW performed the experiments and analyzed the data. All authors contributed to the analysis and supported the manuscript discussion. All authors read and approved the final manuscript.

Funding information

This study was supported by the National Key R&D Program of China (Project No. 2016YFD0501208), National Natural Science Foundation of China (No. 30700588), and Hubei Provincial Natural Science Foundation of China (Grant No. 2014CFB244).

Compliance with ethical standards

All the experimental procedures were approved and conducted under the guidelines provided by the Institutional Animal Care and Ethics Committee of Huazhong Agricultural University, Wuhan, China.

Conflict of interest

The authors declare that they have no competing interests.

Supplementary material

11356_2018_1758_MOESM1_ESM.docx (14 kb)
ESM 1 (DOCX 13.7 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Liu Xueting
    • 1
  • Mujeeb Ur Rehman
    • 1
  • Khalid Mehmood
    • 1
    • 2
  • Shucheng Huang
    • 1
  • Xinxin Tian
    • 1
  • Xiaoxing Wu
    • 1
  • Donghai Zhou
    • 1
  1. 1.College of Veterinary MedicineHuazhong Agricultural UniversityWuhanPeople’s Republic of China
  2. 2.University College of Veterinary & Animal SciencesIslamia University of BahawalpurPunjabPakistan

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