Environmental Science and Pollution Research

, Volume 25, Issue 12, pp 11527–11535 | Cite as

Genotoxicity of spent pot liner as determined with the zebrafish (Danio rerio) experimental model

  • Tássia F. D. Castro
  • Isadora M. Paiva
  • Aline F. S. Carvalho
  • Isadora L. Assis
  • Marcel J. Palmieri
  • Larissa F. Andrade-Vieira
  • Silvana Marcussi
  • Luis D. Solis-Murgas
Research Article


Spent pot liner (SPL) is a solid waste generated during the primary smelting of aluminum, and its toxicity is attributed to the presence of fluoride, cyanide, and aluminum salts, which can be leached into aquatic ecosystems. Since the effects of this waste on aquatic life forms have not yet been investigated, the objective of our study was to evaluate the toxicity of simulated leachates of SPL on zebrafish (Danio rerio). Animals were exposed to 0 (control), 0.32, 0.64, or 0.95 g L−1 of SPL for 24, 72, and 96 h, and genotoxicity was accessed through micronucleus and comet assays. All of the tested treatments induced DNA fragmentation, and the observed frequency of micronuclei and damaged nucleoids generally increased with increasing SPL concentration. The highest frequency of micronuclei (3.3 per 3000 erythrocytes) was detected after 96 h of exposure with 0.95 g L−1 SPL. In the comet assay, nucleoids classified with highest level of damage in relation to the control were observed principally after 24 and 96 h of exposure. The data obtained in this study confirm the genotoxicaction and mutagenic potential of SPL and indicate that open-air deposits of the waste material could represent a health risk to humans and ecosystems alike.


Comet assay Micronucleus assay Gill cells Aluminum DNA fragmentation 



We thank Pedro Henrique Cesar for support in the formatting of Fig. 4.

Funding information

This work was supported by the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), the Coordenação de Aperfeiçoamento de Nível Superior (CAPES), and the Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG).

Compliance with ethical standards

The handling of animals and the experiments conducted with them were carried out in strict accordance with institutional (Animal Experimentation Ethics Committee of the Federal University of Lavras (UFLA), Lavras, MG, Brazil) and national (National Council for Control of Animal Experimentation (CONCEA)) guidelines for the care and use of laboratory animals.

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Tássia F. D. Castro
    • 1
  • Isadora M. Paiva
    • 2
  • Aline F. S. Carvalho
    • 1
  • Isadora L. Assis
    • 1
  • Marcel J. Palmieri
    • 3
  • Larissa F. Andrade-Vieira
    • 3
  • Silvana Marcussi
    • 4
  • Luis D. Solis-Murgas
    • 1
  1. 1.Departamento de Medicina VeterináriaUniversidade Federal de LavrasLavrasBrazil
  2. 2.Departamento de Ciências Biológicas, Setor de GenéticaUniversidade Federal de Minas GeraisBelo HorizonteBrazil
  3. 3.Departamento de Ciências BiológicasUniversidade Federal de LavrasLavrasBrazil
  4. 4.Departamento de QuímicaUniversidade Federal de LavrasLavrasBrazil

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