Environmental Science and Pollution Research

, Volume 25, Issue 10, pp 10058–10068 | Cite as

Cd and Cu accumulation, translocation and tolerance in Populus alba clone (Villafranca) in autotrophic in vitro screening

  • Morena Marzilli
  • Patrick Di Santo
  • Giuseppe Palumbo
  • Lucia Maiuro
  • Bruno Paura
  • Roberto Tognetti
  • Claudia Cocozza
Research Article


The present study investigated accumulation, translocation and tolerance of autotrophic Populus alba clone “Villafranca” in response to excess concentrations of cadmium (Cd) and copper (Cu) provided to the plants. For this purpose, increasing concentrations of Cd (0, 5, 50 and 250 μM) and Cu (0, 5, 50, 250 and 500 μM) were administered to the growth medium in which micropropagated poplar plantlets were exposed to metal treatments for 15 days. Filter bags, instead of the conventional in vitro screening, were applied to improve the experimental design. Results showed that Cd and Cu increased in shoots and roots at increasing metal concentration in the medium. The highest Cd content was found in leaves, while the highest Cu content was found in roots. In “Villafranca”, Cu showed toxic effects on the development of the seedlings, especially at the highest concentrations, reducing plant dry mass. However, the tolerance index (Ti) indicated good tolerance in this clone under exposure to excess metal concentrations, whereas plants had higher translocation factor (Tf). We recommend in vitro selection of tolerant genotypes, aimed at providing early indication on accumulation potentiality and tolerance capability in research on plant sensitivity to excess heavy metal concentrations.


Pollution Cadmium Copper Phytoremediation White poplar Micropropagation Autotrophy 

Supplementary material

11356_2018_1299_MOESM1_ESM.docx (47 kb)
Table S1 (DOCX 46 kb).


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Morena Marzilli
    • 1
  • Patrick Di Santo
    • 1
  • Giuseppe Palumbo
    • 1
  • Lucia Maiuro
    • 1
  • Bruno Paura
    • 1
  • Roberto Tognetti
    • 1
  • Claudia Cocozza
    • 2
  1. 1.Dipartimento Agricoltura, Ambiente e AlimentiUniversità degli Studi del MoliseCampobassoItaly
  2. 2.Consiglio Nazionale delle Ricerche (CNR)Istituto per la Protezione Sostenibile delle Piante (IPSP)Sesto FiorentinoItaly

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