Environmental Science and Pollution Research

, Volume 25, Issue 10, pp 9697–9707 | Cite as

Plasmas ozone inactivation of Legionella in deionized water and wastewater

  • Jun Li
  • Xuebin Li
  • Kunquan Li
  • Tao Tao
Research Article


The results show that ozone concentration determination using ultraviolet spectrophotometry (UV-2450) at 258 nm is easier than using indigo method at 600 nm. A strong linear relationship was found between purge time and O3 concentration in deionized water. Ozone concentration can be predicted in deionized water. A higher O3 flow rate or lower temperature led to a higher O3 concentration. Ozone concentration was stable in 60 min, so that ozone self-decomposition could be ignored at ozone concentrations below 0.4 mg L−1. A higher temperature led to a higher inactivation efficiency and rate, and that a lower temperature led to a lower ozone decay rate and inactivation efficiency even if ozone solubility increased when temperature decreased. The fastest inactivation rate occurred before c0t = 165 μg L−1 s, but the inactivation rate decreased after c0t = 165 μg L−1 s with tail phenomena. The tail phenomena were clearly observed and may be caused by oxidization of lipopolysaccharides (LPS), cell membrane, etc. The activation energy Ea = 55,404 ± 0.3 J mol−1 were obtained for Legionella inactivation with ozone in deionized water. Ozone maximum decay rate was positively proportional to COD concentration. COD impacted on ozone concentration seriously. Higher COD concentration resulted in higher ozone decay rate. COD could result in ozone concentration decrement rapidly to a steady value in 5 s. Higher initial ozone concentration resulted in higher germ inactivation rate. Higher initial COD concentration resulted in lower Legionella inactivation efficiency. COD was easier to react with ozone than Legionella. The relationship among the initial COD concentrations COD0, the initial O3 concentration c0, and the O3 contact time t necessary for a 99.999% reduction of Legionella in wastewater can be expressed in some equations. O3 disinfection time t necessary for a 99.999% reduction of Legionella can be predicted by Eqs. (10) and (11).

Graphical abstract


Indigo Activation energy Tail phenomena Lipopolysaccharides 



The authors would like to thank Professor Thanh Helen Nguyen and Shaoying Qi for supervision and assistance, and Nora Sadik for English editing. Many thanks for EP Purification, Inc. for supplying ozone generator. This project has been supported by a National Science Foundation of USA CAREER grant to T. H. N. (0954501); the Key Research Items of Zhejiang Educational Committee, China (Grant No. Z201119987); the General Research Items of the Natural Science Foundation of Zhejiang Province, China (Grant No. Y5110280); the Natural Science Foundation of China (Grant No. 51102136); and the Doctoral fund of Ministry of Education of China (Grant No. 20110097120021).


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.School of Environmental Science and EngineeringZhejiang Gongshang UniversityZhejiangChina
  2. 2.Department of Management and Information TechnologyNantong Shipping CollegeNantongChina
  3. 3.College of EngineeringNanjing Agricultural UniversityNanjingChina
  4. 4.School of Environmental Science and EngineeringHuazhong University of Science and TechnologyWuhanChina

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