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Environmental Science and Pollution Research

, Volume 25, Issue 10, pp 9342–9350 | Cite as

The acute effects of erythromycin and oxytetracycline on enhanced biological phosphorus removal system: shift in bacterial community structure

  • Zhetai Hu
  • Peide Sun
  • Jingyi Han
  • Ruyi Wang
  • Liang Jiao
  • Pengfei Yang
  • Jing Cai
Research Article

Abstract

Since extensive application, an increasing amount of antibiotics has been released into wastewater treatment plants. In this study, the enhanced biological phosphorus removal (EBPR) system was fed with synthetic wastewater containing erythromycin (ERY) and oxytetracycline (OTC) for 7 days to evaluate the variations of solution ortho-P (SOP), volatile fatty acid (VFA), poly-bhydroxyalkanoates (PHAs), specific oxygen uptake rater (SOUR), and microbial community in the EBPR system. The obtained results showed that the P-removal efficiency decreased to 0.0%, and at the end of the experiment, only less than 20% of the VFA could be consumed. Besides, the variable processes of P and PHAs were destroyed. Moreover, to better grasp the inhibitory mechanism of antibiotics, microbial community compositions of activated sludge sampled in all reactors were investigated by high-throughput sequencing techniques. Results of comparative and evolutionary analysis revealed that high concentrations (5 and 10 mg/L) of ERY and OTC could seriously shift microbial communities, while combined antibiotics could induce more. Additionally, Accumulibacter and Competibacter were two primary microorganisms at the genus level in the EBPR system. Accumulibacter decreased seriously for exposure to antibiotics, while Competibacter increased in all experimental reactors especially in combined antibiotics reactor.

Keywords

Enhanced biological phosphorus removal Erythromycin Oxytetracycline Combined antibiotics effect Microbial community composition 

Notes

Funding information

The study was financially supported by the National Natural Science Foundation of China (No. 21606196), Major Scientific and Technological Project of Zhejiang Province (No. 2014C03002), Scientific Research Fund of Zhejiang Provincial Education Department (No. Y201635678), and Scientific Research Fund of Zhejiang Provincial Education Department (No. Y201635678).

Supplementary material

11356_2018_1221_MOESM1_ESM.docx (1.2 mb)
ESM 1 (DOCX 1212 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Zhetai Hu
    • 1
  • Peide Sun
    • 1
  • Jingyi Han
    • 1
  • Ruyi Wang
    • 1
  • Liang Jiao
    • 1
  • Pengfei Yang
    • 1
  • Jing Cai
    • 1
  1. 1.School of Environmental Science and EngineeringZhejiang Gongshang UniversityHangzhouChina

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