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Teratogenic effects induced by chitosan oligosaccharide in Wistar female rat Rattus norvegicus

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Abstract

The aim of this research is to investigate the teratogenic effects of chitosan oligosaccharide in Wistar female rats (Rattus norvegicus). Chitosan LD50 value was calculated by probit analysis. High dose, 1/10 LD50 which equal to 150 mg/kg body weight, and low dose, 1/30 LD50 which equal to 50 mg/kg body weight, were administrated orally to Wistar female rats to examine the teratogenic effect during organogenesis period from 6th day to 15th day of gestation. Treated and control rats were sacrificed and their foetuses were examined for external, skeletal and visceral anomalies, number and length of foetuses and their weights. Obtained results showed toxicity and teratogenic effects of chitosan on treated rats and their progenies, i.e. high fetal mortality, offspring’s weight and length reduction, and high incidence of fetal external, skeletal and visceral abnormalities. This suggested that chitosan is a teratogenic compound, restricted to current results from orally treated Wistar rats.

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Authors and Affiliations

  1. Pesticides Department, Faculty of Agriculture, Menoufia University, Shibin El Kom, Egypt

    Amal Abdel Aziz Eisa, Gamal El-Sayed Aboelghar, Ibrahim Mahmoud Ammar & Samah Saied Arafa

  2. Clinical Pathology Department, Faculty of Medicine, Cairo University, Giza, Egypt

    Hala Gabr Metwally

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  1. Amal Abdel Aziz Eisa
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  2. Gamal El-Sayed Aboelghar
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  3. Ibrahim Mahmoud Ammar
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  5. Samah Saied Arafa
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Correspondence to Samah Saied Arafa.

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Eisa, A.A.A., Aboelghar, G.ES., Ammar, I.M. et al. Teratogenic effects induced by chitosan oligosaccharide in Wistar female rat Rattus norvegicus. Environ Sci Pollut Res 25, 9371–9379 (2018). https://doi.org/10.1007/s11356-018-1199-8

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