Environmental Science and Pollution Research

, Volume 25, Issue 11, pp 10362–10370 | Cite as

An enhancement of antimicrobial efficacy of biogenic and ceftriaxone-conjugated silver nanoparticles: green approach

  • Rajasree Shanmuganathan
  • Davoodbasha MubarakAli
  • Desika Prabakar
  • Harshiny Muthukumar
  • Nooruddin Thajuddin
  • Smita S. Kumar
  • Arivalagan Pugazhendhi
Plant-borne compounds and nanoparticles: challenges for medicine, parasitology and entomology


Of the various methods explored for the synthesis of nanoparticles, biogenesis of silver nanoparticles (AgNPs) received great attention due to their versatile properties. In this report, Daucus carota extract was used for the synthesis of AgNPs and ceftriaxone was conjugated with AgNPs to enhance their antimicrobial efficacy. The conjugated and unconjugated AgNPs were characterized by adopting UV-Vis spectroscopy, FTIR, AFM, DLS, and TEM, which revealed the SPR peak at 420 nm and spherical shaped nanoparticles of 20 nm size, respectively. The antimicrobial efficacies of the unconjugated AgNPs and ceftriaxone-conjugated AgNPs were tested against ceftriaxone-resistant human pathogens, Bacillus cereus, Staphylococcus aureus, Klebsiella pneumoniae, and Pseudomonas aeruginosa. The ceftriaxone-conjugated AgNPs showed high inhibitory action (23 mm) than the unconjugated AgNPs (18 mm) at the concentration of 50 μg/mL. Both the unconjugated and ceftriaxone-conjugated AgNPs were found to be non-toxic on EAC cells at 50 μg/mL. The dose-dependent cytotoxic activities were observed on increasing the concentration of the AgNPs. The ceftriaxone-conjugated AgNPs showed high activity than the unconjugated AgNPs. The enhanced activity could be useful to treat ceftriaxone-resistant human pathogens.


Biogenesis AgNPs Ceftriaxone Antimicrobials Cytotoxic 



The authors would like to acknowledge DST-FIST for providing the instrumentation facilities for characterization studies. Department of Biotechnology (DBT, Govt. Of India) is gratefully acknowledged for NRMC-F.


  1. Baüer AW, Kirby WM, Sherris JC, Turck M (1966) Antibiotic susceptibility testing by a standardized single disk method. J Clin Pathol 45:493–496CrossRefGoogle Scholar
  2. Christy AJ, Umadevi M (2012) Synthesis and characterization of monodispersed silver nanoparticles. Adv Nat Sci Nanosci Nanotechnol 3:035013CrossRefGoogle Scholar
  3. Durán N, Marcato PD, Alves OL et al (2005) Mechanistic aspects of biosynthesis of silver nanoparticles by several Fusarium oxysporum strains. J Nanobiotechnol 3:8CrossRefGoogle Scholar
  4. Dutta PP, Bordoloi M, Gogoi K et al (2017) Antimalarial silver and gold nanoparticles: green synthesis, characterization and in vitro study. Biomed Pharmacother 91:567–580CrossRefGoogle Scholar
  5. Gomaa EZ (2017) Antimicrobial, antioxidant and antitumor activities of silver nanoparticles synthesized by Allium cepa extract: a green approach. J Genet Eng Biotechnol. doi: 10.1016/j.jgeb.2016.12.002
  6. Gopinath V, MubarakAli D, Priyadarshini S, Meera Priyadharsshini S, Thajuddin N, Velusamy P (2012) Biosynthesis of silver nanoparticles from Tribulus terrestris and its antimicrobial activity: a novel biological approach. Colloids Surf B: Biointerfaces 96:69–74CrossRefGoogle Scholar
  7. Gopinath V, Priyadarshini S, Loke MF et al (2015) Biogenic synthesis, characterization of antibacterial silver nanoparticles and its cell cytotoxicity. Arab J Chem. doi: 10.1016/j.arabjc.2015.11.011
  8. Harshiny M, Matheswaran M, Arthanareeswaran G et al (2015) Enhancement of antibacterial properties of silver nanoparticles–ceftriaxone conjugate through Mukia maderaspatana leaf extract mediated synthesis. Ecotoxicol Environ Saf 121:135–141CrossRefGoogle Scholar
  9. Iravani S, Korbekandi H, Mirmohammadi SV, Zolfaghari B (2014) Synthesis of silver nanoparticles: chemical, physical and biological methods. Res Pharm Sci 9:385–406Google Scholar
  10. Jacob SJP, Finub JS, Narayanan A (2012) Synthesis of silver nanoparticles using Piper longum leaf extracts and its cytotoxic activity against Hep-2 cell line. Colloids Surf B Biointerfaces 91:212–214CrossRefGoogle Scholar
  11. Jeyaraj M, Sathishkumar G, Sivanandhan G, MubarakAli D et al (2013) Biogenic silver nanoparticles for cancer treatment: an experimental report. Colloids Surf B Biointerfaces 106:86–92CrossRefGoogle Scholar
  12. Kanmani P, Lim ST (2013) Synthesis and structural characterization of silver nanoparticles using bacterial exopolysaccharide and its antimicrobial activity against food and multidrug resistant pathogens. Process Biochem 48:1099–1106CrossRefGoogle Scholar
  13. Liu L, Yang J, Xie J et al (2013) The potent antimicrobial properties of cell penetrating peptide-conjugated silver nanoparticles with excellent selectivity for gram-positive bacteria over erythrocytes. Nano 5:3834–3840Google Scholar
  14. Logeswari P, Silambarasan S, Abraham J (2013) Ecofriendly synthesis of silver nanoparticles from commercially available plant powders and their antibacterial properties. Scientia Iranica 20:1049–1054Google Scholar
  15. Lok C-N, Ho C-M, Chen R et al (2006) Proteomic analysis of the mode of antibacterial action of silver nanoparticles. J Proteome Res 5:916–924CrossRefGoogle Scholar
  16. Maráková N, Humpolíček P, Kašpárková V et al (2017) Antimicrobial activity and cytotoxicity of cotton fabric coated with conducting polymers, polyaniline or polypyrrole, and with deposited silver nanoparticles. Appl Surf Sci 396:169–176CrossRefGoogle Scholar
  17. Mohanty S, Mishra S, Jena P et al (2012) An investigation on the antibacterial, cytotoxic, and antibiofilm efficacy of starch-stabilized silver nanoparticles. Nanomed Nanotech Biol Med 8:916–924CrossRefGoogle Scholar
  18. MubarakAli D, Thajuddin N, Jeganathan K, Gunasekaran M (2011) Plant extract mediated synthesis of silver and gold nanoparticles and its antibacterial activity against clinically isolated pathogens. Colloids Surf B Biointerfaces 85:360–365CrossRefGoogle Scholar
  19. MubarakAli D, Sang-Yul L, Seong-Cheol K, Jung-Wan K (2015) One-step synthesis of cellulose/silver nanobiocomposites using a solution plasma process and characterization of their broad spectrum antimicrobial efficacy. RSC Adv 5:35052–35060CrossRefGoogle Scholar
  20. MubarakAli D, Seong-Cheol K, Sang-Yul L, Jung-Wan K (2016) The facile synthesis of chitosan-based silver nano-biocomposites via a solution plasma process and their potential antimicrobial efficacy. Arch Biochem Biophys 605:49–58CrossRefGoogle Scholar
  21. Muthu K, Priya S (2017) Green synthesis, characterization and catalytic activity of silver nanoparticles using Cassia auriculata flower extract separated fraction. Spectrochim Acta Mol Biomol Spectrosc 179:66–72CrossRefGoogle Scholar
  22. Ostaszewska T, Chojnacki M, Kamaszewski M, Sawosz-Chwalibóg E (2016) Histopathological effects of silver and copper nanoparticles on the epidermis, gills, and liver of Siberian sturgeon. Environ Sci Pollut Res Int 23:1621–1633CrossRefGoogle Scholar
  23. Pugazhendhi S, Kirubha E, Palanisamy PK, Gopalakrishnan R (2015) Synthesis and characterization of silver nanoparticles from Alpinia calcarata by Green approach and its applications in bactericidal and nonlinear optics. Appl Surf Sci 357:1801–1808CrossRefGoogle Scholar
  24. Pugazhendhi S, Sathya P, Palanisamy PK, Gopalakrishnan R (2016) Synthesis of silver nanoparticles through green approach using Dioscorea alata and their characterization on antibacterial activities and optical limiting behavior. J Photochem Photobiol B 159:155–160CrossRefGoogle Scholar
  25. Rajakumar G, Abdul Rahuman A (2011) Larvicidal activity of synthesized silver nanoparticles using Eclipta prostrata leaf extract against filariasis and malaria vectors. Acta Trop 118:196–203CrossRefGoogle Scholar
  26. Ramkumar VS, Pugazhendhi A, Gopalakrishnan K et al (2017) Biofabrication and characterization of silver nanoparticles using aqueous extract of seaweed Enteromorpha compressa and its biomedical properties. Biotechnol Rep 14:1–7CrossRefGoogle Scholar
  27. Sahana R, Daniel SCGK, Sankar SG et al (2014) Formulation of bactericidal cold cream against clinical pathogens using Cassia auriculata flower extract-synthesized Ag nanoparticles. Green Chem Lett Rev 7:64–72CrossRefGoogle Scholar
  28. Salem JK, El-Nahhal IM, Najri BA et al (2016) Effect of anionic surfactants on the surface plasmon resonance band of silver nanoparticles: determination of critical micelle concentration. J Mol Liq 223:771–774CrossRefGoogle Scholar
  29. Sangwoo N, MubarakAli D, Jungwan K (2016) Characterization of alginate/silver nanobiocomposites synthesized by solution plasma process and their antimicrobial properties. J Nanomater 4712813:9Google Scholar
  30. Saratale GD, Saratale RG, Benelli G et al (2017) Anti-diabetic potential of silver nanoparticles synthesized with Argyreia nervosa leaf extract high synergistic antibacterial activity with standard antibiotics against foodborne bacteria. J Clust Sci 28:1709–1727CrossRefGoogle Scholar
  31. Sarkar S, Jana AD, Samanta SK, Mostafa G (2007) Facile synthesis of silver nano particles with highly efficient anti-microbial property. Polyhedron 26:4419–4426CrossRefGoogle Scholar
  32. Shankar PD, Shobana S, Karuppusamy I et al (2016) A review on the biosynthesis of metallic nanoparticles (gold and silver) using bio-components of microalgae: formation mechanism and applications. Enzym Microb Technol 95:28–44CrossRefGoogle Scholar
  33. Shrivastava S, Bera T, Roy A et al (2007) Characterization of enhanced antibacterial effects of novel silver nanoparticles. Nanotechnology 18:225103CrossRefGoogle Scholar
  34. Singh D, Rathod V, Ninganagouda S et al (2014) Optimization and characterization of silver nanoparticle by endophytic fungi Penicillium sp. isolated from Curcuma longa (turmeric) and application studies against MDR E. coli and S. aureus. Bioinorg Chem Appl 2014:e408021Google Scholar
  35. Sondi I, Salopek-Sondi B (2004) Silver nanoparticles as antimicrobial agent: a case study on E. coli as a model for gram-negative bacteria. J Colloid Interface Sci 275:177–182CrossRefGoogle Scholar
  36. Sperling RA, Parak WJ (2010) Surface modification, functionalization and bioconjugation of colloidal inorganic nanoparticles. Philos Trans R Soc Lond A 368:1333–1383CrossRefGoogle Scholar
  37. Su Y, Zhao L, Meng F et al (2017) Silver nanoparticles decorated lipase-sensitive polyurethane micelles for on-demand release of silver nanoparticles. Colloids Surf B: Biointerfaces 152:238–244CrossRefGoogle Scholar
  38. Suman TY, Radhika Rajasree SR, Kanchana A, Elizabeth SB (2013) Biosynthesis, characterization and cytotoxic effect of plant mediated silver nanoparticles using Morinda citrifolia root extract. Colloids Surf B: Biointerfaces 106:74–78CrossRefGoogle Scholar
  39. Thiel J, Pakstis L, Buzby S et al (2007) Antibacterial properties of silver-doped titania. Small 3:799–803CrossRefGoogle Scholar
  40. Venugopal K, Rather HA, Rajagopal K et al (2017) Synthesis of silver nanoparticles (Ag NPs) for anticancer activities (MCF 7 breast and A549 lung cell lines) of the crude extract of Syzygium aromaticum. J Photochem Photobiol B Biol 167:282–289CrossRefGoogle Scholar
  41. Vijayan SR, Santhiyagu P, Ramasamy R et al (2016) Seaweeds: a resource for marine bionanotechnology. Enzym Microb Technol 95:45–57CrossRefGoogle Scholar
  42. Yohannan Panicker C, Tresa Varghese H, Philip D (2006) FT-IR, FT-Raman and SERS spectra of vitamin C. Spectrochim Acta Mol Biomol Spectrosc 65:802–804CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2017

Authors and Affiliations

  1. 1.CoRx Life sciences and Pharmaceutical (CLAP) Private LimitedTiruchirappalliIndia
  2. 2.National Repository for Microalgae and Cyanobacteria - Freshwater (DBT), Department of MicrobiologyBharathidasan UniversityTiruchirappalliIndia
  3. 3.Anna UniversityChennaiIndia
  4. 4.Department of BiotechnologyPeriyar Maniammai UniversityThanjavurIndia
  5. 5.Department of Environmental Science and EngineeringGuru Jambheshwar University of Science and TechnologyHisarIndia
  6. 6.Green Processing, Bioremediation and Alternative Energies Research Group (GPBAE), Faculty of Environment and Labour SafetyTon Duc Thang UniversityHo Chi Minh CityVietnam

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