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Environmental Science and Pollution Research

, Volume 25, Issue 11, pp 10362–10370 | Cite as

An enhancement of antimicrobial efficacy of biogenic and ceftriaxone-conjugated silver nanoparticles: green approach

  • Rajasree Shanmuganathan
  • Davoodbasha MubarakAli
  • Desika Prabakar
  • Harshiny Muthukumar
  • Nooruddin Thajuddin
  • Smita S. Kumar
  • Arivalagan Pugazhendhi
Plant-borne compounds and nanoparticles: challenges for medicine, parasitology and entomology

Abstract

Of the various methods explored for the synthesis of nanoparticles, biogenesis of silver nanoparticles (AgNPs) received great attention due to their versatile properties. In this report, Daucus carota extract was used for the synthesis of AgNPs and ceftriaxone was conjugated with AgNPs to enhance their antimicrobial efficacy. The conjugated and unconjugated AgNPs were characterized by adopting UV-Vis spectroscopy, FTIR, AFM, DLS, and TEM, which revealed the SPR peak at 420 nm and spherical shaped nanoparticles of 20 nm size, respectively. The antimicrobial efficacies of the unconjugated AgNPs and ceftriaxone-conjugated AgNPs were tested against ceftriaxone-resistant human pathogens, Bacillus cereus, Staphylococcus aureus, Klebsiella pneumoniae, and Pseudomonas aeruginosa. The ceftriaxone-conjugated AgNPs showed high inhibitory action (23 mm) than the unconjugated AgNPs (18 mm) at the concentration of 50 μg/mL. Both the unconjugated and ceftriaxone-conjugated AgNPs were found to be non-toxic on EAC cells at 50 μg/mL. The dose-dependent cytotoxic activities were observed on increasing the concentration of the AgNPs. The ceftriaxone-conjugated AgNPs showed high activity than the unconjugated AgNPs. The enhanced activity could be useful to treat ceftriaxone-resistant human pathogens.

Keywords

Biogenesis AgNPs Ceftriaxone Antimicrobials Cytotoxic 

Notes

Acknowledgements

The authors would like to acknowledge DST-FIST for providing the instrumentation facilities for characterization studies. Department of Biotechnology (DBT, Govt. Of India) is gratefully acknowledged for NRMC-F.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2017

Authors and Affiliations

  1. 1.CoRx Life sciences and Pharmaceutical (CLAP) Private LimitedTiruchirappalliIndia
  2. 2.National Repository for Microalgae and Cyanobacteria - Freshwater (DBT), Department of MicrobiologyBharathidasan UniversityTiruchirappalliIndia
  3. 3.Anna UniversityChennaiIndia
  4. 4.Department of BiotechnologyPeriyar Maniammai UniversityThanjavurIndia
  5. 5.Department of Environmental Science and EngineeringGuru Jambheshwar University of Science and TechnologyHisarIndia
  6. 6.Green Processing, Bioremediation and Alternative Energies Research Group (GPBAE), Faculty of Environment and Labour SafetyTon Duc Thang UniversityHo Chi Minh CityVietnam

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