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Formulation and characterization of garlic (Allium sativum L.) essential oil nanoemulsion and its acaricidal activity on eriophyid olive mites (Acari: Eriophyidae)

  • Plant-borne compounds and nanoparticles: challenges for medicine, parasitology and entomology
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Abstract

Green and nanoacaricides including essential oil (EO) nanoemulsions are important compounds to provide new, active, safe acaricides and lead to improvement of avoiding the risk of synthetic acaricides. This study was carried out for the first time on eriophyid mites to develop nanoemulsion of garlic essential oil by ultrasonic emulsification and evaluate its acaricidal activity against the two eriophyid olive mites Aceria oleae Nalepa and Tegolophus hassani (Keifer). Acute toxicity of nanoemulsion was also studied on male rats. Garlic EO was analyzed by gas chromatography–mass spectrometry (GC-MS), and the major compounds were diallyl sulfide (8.6%), diallyl disulfide (28.36%), dimethyl tetrasulfide (15.26%), trisulfide,di-2-propenyl (10.41%), and tetrasulfide,di-2-propenyl (9.67%). Garlic oil nanoemulsion with droplet size 93.4 nm was formulated by ultrasonic emulsification for 35 min. Emulsification time and oil and surfactant ratio correlated to the emulsion droplet size and stability. The formulated nanoemulsion showed high acaricidal activity against injurious eriophyid mites with LC50 298.225 and 309.634 μg/ml, respectively. No signs of nanoemulsion toxicity were noted in treating rats; thus, it may be considered non-toxic to mammals. Stability of garlic oil nanoemulsion, high acaricidal activity, and the absence of organic toxic solvents make the formulation that may be a possible acaricidal product. Results suggest the possibility of developing suitable natural nanoacaricide from garlic oil.

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References

  • Abbassy MA et al (2014) Adverse biochemical effects of various pesticides on sprayers of cotton fields in El-Behira Governorate, Egypt. Biomed Aging Pathol ​4(3):251–256

  • Abou-Awad BA, Metwally AM, A.-A.M. (2005) Environmental management and biological aspects of two erio-phyid olive mites in Egypt: Aceria oleae and Tegolophus hassani. Z Pflanzenkrankh Pflanzenschutz 112(3):287–303

    Google Scholar 

  • Adil B et al (2015) The study of the insecticidal effect of Nigella sativa essential oil against Tuta absoluta larvae. Int J Sci Technol Res 4(10):88–90. Available at: www.ijstr.org. Accessed 15 Nov 2016

  • Aktar MW, Sengupta D, Chowdhury A (2009) Impact of pesticides use in agriculture: their benefits and hazards. Interdiscip Toxicol 2(1):1–12. Available at: http://www.ncbi.nlm.nih.gov/pubmed/21217838 [Accessed December 15, 2016]. https://doi.org/10.2478/v10102-009-0001-7

    Article  Google Scholar 

  • Anjali C et al (2012) Neem oil (Azadirachta indica) nanoemulsion—a potent larvicidal agent against Culex quinquefasciatus. Pest Manag Sci 68(2):158–163. https://doi.org/10.1002/ps.2233

    Article  CAS  Google Scholar 

  • Araújo FA, Kelmann RG, Araújo BV, Finatto RB, Teixeira HF, Koester LS (2011) Development and characterization of parenteral nanoemulsions containing thalidomide. Eur J Pharm Sci 42(3):238–245. https://doi.org/10.1016/j.ejps.2010.11.014

    Article  Google Scholar 

  • Attia S, Grissa KL, Mailleux AC, Lognay G, Heuskin S, Mayoufi S, Hance T (2012) Effective concentrations of garlic distillate (Allium sativum) for the control of Tetranychus urticae (Tetranychidae). J Appl Entomol 136(4):302–312. https://doi.org/10.1111/j.1439-0418.2011.01640.x

    Article  Google Scholar 

  • Bakr EM (2000) LdP line software. URL: http://www.ehabsoft.com/ldpline/

  • Burt S (2004) Essential oils: their antibacterial properties and potential applications in foods—a review. 94:223–253

  • Çalmaşur Ö, Aslan I, Şahin F (2006) Insecticidal and acaricidal effect of three Lamiaceae plant essential oils against Tetranychus urticae Koch and Bemisia tabaci Genn. Ind Crop Prod 23(2):140–146. https://doi.org/10.1016/j.indcrop.2005.05.003

    Article  Google Scholar 

  • Chanda SM, Mortensen SR, Moser VC, Padilla S (1997) Tissue-specific effects of chlorpyrifos on carboxylesterase and cholinesterase activity in adult rats: an in vitro and in vivo comparison. Fundam Appl Toxicol 38(2):148–157

  • Chintkuntla (2015) Toxicity of plant essential oils to Tetranychus urticae (Acari: Tetranychidae) and Phytoseiulus persimilis (Acari: Phytoseiidae). J Econ Entomol 97(2):553–558

    Google Scholar 

  • Choi W, et al. (2006) Fumigant toxicities of essential oils and monoterpenes against Lycoriella mali adults. 25:398–401

  • Diaz S, Espinosa S, Brignole EA (2005) Citrus peel oil deterpenation with supercritical fluids optimal process and solvent cycle design. 35(896):49–61

  • Duarte JL, Amado JRR, Oliveira AEMFM, Cruz RAS, Ferreira AM, Souto RNP, Falcão DQ, Carvalho JCT, Fernandes CP (2015) Evaluation of larvicidal activity of a nanoemulsion of Rosmarinus officinalis essential oil. Braz J Pharmacogn 25(2):189–192. https://doi.org/10.1016/j.bjp.2015.02.010

    Article  CAS  Google Scholar 

  • Dziri S et al (2013) Composition of garlic essential oil (Allium sativum L.) as influenced by drying method. J Essent Oil Res 26(2):91–96

    Article  Google Scholar 

  • El-zemity SR, Rezk HA, Zaitoon AA (2006) Acaricidal activity of some essential oils and their monoterpenoidal constituents against the parasitic bee mites. Varroa Destructor (Acari: Varroidae) 2(11):1032–1036

    Google Scholar 

  • Environews Forum (1999) Killer environment. Environ Health Perspect 107:A62

  • Fernandes CP, et al. (2014) Development of an insecticidal nanoemulsion with Manilkara subsericea (Sapotaceae) extract, 1–9

  • Finney DJ (1971) Probit analysis statistical treatment of the sigmoid response curve (Book, 1971) WorldCat. Cambridge Univ. Press, Cambridge

    Google Scholar 

  • Gara EAO, Hill DJ (2000) Activities of garlic oil, garlic powder, and their diallyl constituents against Helicobacter pylori. Appl Environ Microbiol 66(5):2269–2273

    Article  Google Scholar 

  • Ghosh V (2013) Formulation and characterization of plant essential oil based nanoemulsion: evaluation of its larvicidal activity against Aedes aegypti †. Asian J Chem 25(March):18–20

    Google Scholar 

  • Ghosh V, Saranya S, Mukherjee A, Chandrasekaran N (2013) Cinnamon oil nanoemulsion formulation by ultrasonic emulsification: investigation of its bactericidal activity. J Nanosci Nanotechnol 13(May):114–122. https://doi.org/10.1166/jnn.2013.6701

    Article  CAS  Google Scholar 

  • Ghosh V, Mukherjee A, Chandrasekaran N (2014) Optimization of process parameters to formulate nanoemulsion by spontaneous emulsification: evaluation of larvicidal activity against Culex quinquefasciatus larva. BioNanoScience 4(2):157–165. https://doi.org/10.1007/s12668-014-0131-z

    Article  Google Scholar 

  • Goel A, Dani V, Dhawan DK (2005) Protective effects of zinc on lipid peroxidation, antioxidant enzymes and hepatic histoarchitecture in chlorpyrifos-induced toxicity. Chem Biol Interact 156:131–140

    Article  CAS  Google Scholar 

  • Golemanov K, Tcholakova S, Denkov ND, Gurkov T (2006) Selection of surfactants for stable paraffin-in-water dispersions, undergoing solid-liquid transition of the dispersed particles. Langmuir 22(8):3560–3569. https://doi.org/10.1021/la053059y

    Article  CAS  Google Scholar 

  • Gupta R, Sharma A, Maina P, Shukla RN (2014) Study of chemical composition of garlic oil and comparative analysis of cotrimoxazole in response to in vitro antibacterial activity. Int Res J Pharm 5(2):97–101. Available at: http://www.irjponline.com/admin/php/uploads/2110_pdf.pdf. https://doi.org/10.7897/2230-8407.050220

    Article  Google Scholar 

  • Habluetzel A, et al (2006) Impact of the botanical insecticide Neem Azal 1 on survival and reproduction of the biting louse Damalinia limbata on angora goats, 1–10

  • Hala MI (2012) Activity of essential oil of lemongrass, Chymbopogon citratus (DC) Stapf against Tetranychus urticae Koch. 3(1):43–51

  • Han J, Kim SI, Choi BR, Lee SG, Ahn YJ (2011) Fumigant toxicity of lemon eucalyptus oil constituents to acaricide-susceptible and acaricide-resistant Tetranychus urticae. Pest Manag Sci 67(12):1583–1588. https://doi.org/10.1002/ps.2216

    Article  CAS  Google Scholar 

  • International Olive Council (2007) Production techniques in olive growing. ​http://www.internationaloliveoil.org/store/view/51-production-techniques-in-olive-growing-english

  • Jeppson LR, Keifer HH, Baker EW (1975) Mites injurious to economic plants. University of California Press. Available at: http://www.ucpress.edu/op.php?isbn=9780520023819. Accessed 21 Mar 2017

  • Jirovetz L et al (1992) Investigations of volatile constituents of the essential oil of Egyptian garlic (Allium sativum L.) by means of GC-MS and GC-FTIR. Z Lebensmittel-Untersuchung Forschung 194(4):363–365

    Article  CAS  Google Scholar 

  • Kim JW et al (2004) Antimicrobial activity of alk(en)yl sulfides found in essential oils of garlic and onion. Food Sci Biotechnol 13(2):235–239

    CAS  Google Scholar 

  • Kimbaris AC, Siatis NG, Pappas CS, Tarantilis PA, Daferera DJ, Polissiou MG (2006) Quantitative analysis of garlic (Allium sativum) oil unsaturated acyclic components using FT-Raman spectroscopy. Food Chem 94(2):287–295. https://doi.org/10.1016/j.foodchem.2005.01.017

    Article  CAS  Google Scholar 

  • Klang V, Matsko NB, Valenta C, Hofer F (2012) Electron microscopy of nanoemulsions: an essential tool for characterisation and stability assessment. Micron 43(2–3):85–103. https://doi.org/10.1016/j.micron.2011.07.014

    Article  CAS  Google Scholar 

  • Koul O (2004) Biological activity of volatile di-n-propyl disulfide from seeds of neem, Azadirachta indica (Meliaceae), to two species of stored grain pests, Sitophilus oryzae (L.) and Tribolium castaneum (Herbst). J Econ Entomol 97(3):1142–1147

  • Laborda R, Manzano I, Gamón M, Gavidia I, Pérez-Bermúdez P, Boluda R (2013) Effects of Rosmarinus officinalis and Salvia officinalis essential oils on Tetranychus urticae Koch (Acari: Tetranychidae). Ind Crop Prod 48:106–110. https://doi.org/10.1016/j.indcrop.2013.04.011

    Article  CAS  Google Scholar 

  • Mahdi ES, Noor AM, Sakeena MH, Abdullah GZ, Abdulkarim MF, Sattar MA (2011) Formulation and in vitro release evaluation of newly synthesized palm kernel oil esters-based nanoemulsion delivery system for 30% ethanolic dried extract derived from local Phyllanthus urinaria for skin antiaging. Int J Nanomedicine 6:2499–2512. https://doi.org/10.2147/IJN.S22337

    Article  CAS  Google Scholar 

  • Mansour SA, Mossa A-TH (2009) Oxidative damage, biochemical and histopathological alterations in rats exposed to chlorpyrifos and the antioxidant role of zinc. Pestic Biochem Physiol 96:14–23

    Article  Google Scholar 

  • Mansour SA, Mossa A-TH (2011) Adverse effects of exposure to low doses of chlorpyrifos in lactating rats. Toxicol Ind Health 27(3):213–224. Available at: http://www.ncbi.nlm.nih.gov/pubmed/20870695. https://doi.org/10.1177/0748233710384054

    Article  CAS  Google Scholar 

  • Maurhofer M, Schl E (2007) Elsinochrome A production by the bindweed biocontrol fungus Stagonospora convolvuli LA39 does not pose a risk to the environment or the consumer of treated crops. 59:194–205

  • Miresmailli S, Isman MB (2006) Efficacy and persistence of rosemary oil as an acaricide against two spotted spider mite (Acari: Tetranychidae) on greenhouse tomato. J Econ Entomol 99(6):2015–2023. https://doi.org/10.1093/jee/99.6.2015

    Article  Google Scholar 

  • Mnayer D, Fabiano-Tixier AS, Petitcolas E, Hamieh T, Nehme N, Ferrant C, Fernandez X, Chemat F (2014) Chemical composition, antibacterial and antioxidant activities of six essentials oils from the Alliaceae family. Molecules 19(12):20034–20053. https://doi.org/10.3390/molecules191220034

    Article  Google Scholar 

  • Mossa A-TH (2016) Green pesticides: essential oils as biopesticides in insect-pest management. J Environ Sci Technol 9(5):​354–378

  • Mossa ATH, Swelam ES, Mohafrash SMM (2015) Sub-chronic exposure to fipronil induced oxidative stress, biochemical and histotopathological changes in the liver and kidney of male albino rats. Toxicol Rep 2:775–784. https://doi.org/10.1016/j.toxrep.2015.02.009

    Article  CAS  Google Scholar 

  • Mossa A-TH, Abdel Rasoul MA, Mohafrash SMM (2017) Lactational exposure to abamectin induced mortality and adverse biochemical and histopathological effects in suckling pups. Environ Sci Pollut Res 24(11):10150–10165

  • Naher S et al (2014) Comparative studies on physicochemical properties and GC-MS analysis of essential oil of two varieties of Allium sativum Linn ( garlic). Int J Pharm Phytopharmacol Res 4(3):173–175

    Google Scholar 

  • NRCoN, A (2011) Guide for the care and use of laboratory animals. 8th edn

  • Oliveira AEMFM et al (2016) Development of larvicidal nanoemulsion with Pterodon emarginatus Vogel oil. PLoS One 11(1):1–16

    Google Scholar 

  • Orafidiya LO, Oladimeji FA (2002) Determination of the required HLB values of some essential oils. Int J Pharm 237(1–2):241–249. https://doi.org/10.1016/S0378-5173(02)00051-0

    Article  CAS  Google Scholar 

  • Park BS et al. (2002) Insecticidal and acaricidal activity of pipernonaline and piperoctadecalidine derived from dried fruits of Piper longum L. Crop Protection

  • Pontes WJT, Oliveira JCG, Câmara CAG, Lopes ACHR, Gondim Júnior MGC, Oliveira JV, Barros R, Schwartz MOE (2007) Chemical composition and acaricidal activity of the leaf and fruit essential oils of Protium heptaphyllum (Aubl.) Marchand (Burseraceae). Acta Amazon 37(1):103–109. https://doi.org/10.1590/S0044-59672007000100012

    Article  CAS  Google Scholar 

  • Rao PGP, Rao LJ, Raghavan B (1999) Chemical composition of essential oils of garlic (Allium sativum L.) J Spices Aromatic Crops 8(1):41–47

    Google Scholar 

  • Rattan RS (2010) Mechanism of action of insecticidal secondary metabolites of plant origin. Crop Prot 29(9):913–920. https://doi.org/10.1016/j.cropro.2010.05.008

    Article  CAS  Google Scholar 

  • Rattanachaikunsopon P, Phumkhachorn P (2008) Diallyl sulfide content and antimicrobial activity against food-borne pathogenic bacteria of chives (Allium schoenoprasum). Biosci Biotechnol Biochem 72(11):2987–2991

    Article  CAS  Google Scholar 

  • Rhomberg LR, Wolff SK (1998) Empirical scaling of single oral lethal doses across mammalian species based on a large database. Risk Anal 18(6):741–753

  • Ribeiro JC, Ribeiro WLC, Camurça-Vasconcelos ALF, Macedo ITF, Santos JML, Paula HCB, Araújo Filho JV, Magalhães RD, Bevilaqua CML (2014) Efficacy of free and nanoencapsulated Eucalyptus citriodora essential oils on sheep gastrointestinal nematodes and toxicity for mice. Vet Parasitol 204(3-4):243–248. https://doi.org/10.1016/j.vetpar.2014.05.026

    Article  CAS  Google Scholar 

  • Rodrigues E d CR, Ferreira AM, Vilhena JCE, Almeida FB, Cruz RAS, Florentino AC, Souto RNP, Carvalho JCT, Fernandes CP (2014) Development of a larvicidal nanoemulsion with Copaiba (Copaifera duckei) oleoresin. Braz J Pharmacogn 24(6):699–705. https://doi.org/10.1016/j.bjp.2014.10.013

    Article  CAS  Google Scholar 

  • Salvia-Trujillo L, Rojas-Graü MA, Soliva-Fortuny R, Martín-Belloso O (2013) Effect of processing parameters on physicochemical characteristics of microfluidized lemongrass essential oil-alginate nanoemulsions. Food Hydrocoll 30(1):401–407. https://doi.org/10.1016/j.foodhyd.2012.07.004

    Article  CAS  Google Scholar 

  • Samojlik I, Lakić N, Mimica-Dukić N, Đaković-Švajcer K, Božin B (2010) Antioxidant and hepatoprotective potential of essential oils of coriander (Coriandrum sativum L.) and caraway (Carum carvi L.) (Apiaceae). J Agric Food Chem 58(15):8848–8853. https://doi.org/10.1021/jf101645n

    Article  CAS  Google Scholar 

  • Shafiq S et al (2006) Development and bioavailability assessment of ramipril nanoemulsion formulation. Eur J Pharm Biopharm 66(2):227–243

  • Sugumar S et al. (2014) Nanoemulsion of eucalyptus oil and its larvicidal activity against Culex quinquefasciatus. 393–402

  • Swidan MH (2007) Effect of garlic oil and allyl disulfide on protein and amino acid composition of two stored-product adult insects Callosobruchus chinensis (Coleoptera: Bruchidae) and Sitophilus oryzae (Coleoptera: Curculionidae). Sci J Fac Sci Minu-a Univ XXI:91–113

  • Tadros T et al (2004) Formation and stability of nano-emulsions. Adv Colloid Interf Sci 108–109:303–318

    Article  Google Scholar 

  • Walter DE, Proctor HC (1999) Mites: ecology, Evolution, and Behaviour. UNSW Press, Sydney

    Google Scholar 

  • Wang L, Dong J, Chen J, Eastoe J, Li X (2009) Design and optimization of a new self-nanoemulsifying drug delivery system. J Colloid Interface Sci 330(2):443–448. https://doi.org/10.1016/j.jcis.2008.10.077

    Article  CAS  Google Scholar 

  • Wang L, Li X, Zhang G, Dong J, Eastoe J (2007) Oil-in-water nanoemulsions for pesticide formulations. J Colloid Interface Sci 314(1):230–235. https://doi.org/10.1016/j.jcis.2007.04.079

    Article  CAS  Google Scholar 

  • Yang FL, Li XG, Zhu F, Lei CL (2009) Structural characterization of nanoparticles loaded with garlic essential oil and their insecticidal activity against Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae). J Agric Food Chem 57(21):10156–10162. https://doi.org/10.1021/jf9023118

    Article  CAS  Google Scholar 

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  1. Pesticide Chemistry Department, National Research Centre (NRC), 33 El Bohouth Street (former El Tahrir St.), Dokki, P.O. 12622, Giza, Egypt

    Abdel-Tawab H. Mossa & Samia M. M. Mohafrash

  2. Plant Protection Department, National Research Centre (NRC), 33 El Bohouth Street (former El Tahrir St.), Dokki, P.O. 12622, Giza, Egypt

    Sahar I. Afia & Badawi A. Abou-Awad

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  1. Abdel-Tawab H. Mossa
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  2. Sahar I. Afia
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  4. Badawi A. Abou-Awad
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Mossa, AT.H., Afia, S.I., Mohafrash, S.M.M. et al. Formulation and characterization of garlic (Allium sativum L.) essential oil nanoemulsion and its acaricidal activity on eriophyid olive mites (Acari: Eriophyidae). Environ Sci Pollut Res 25, 10526–10537 (2018). https://doi.org/10.1007/s11356-017-0752-1

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