The present study aimed to investigate the effect of combined resistance aerobic exercise training on asprosin and complement C1q tumor necrosis factor-related protein-1 concentrations in men with type 2 diabetes.
Twenty four male patients with type 2 diabetes were randomized to two combined resistance aerobic exercise training group (n = 12) and control group (n = 12). The combined resistance aerobic exercise training was performed within three sessions per week for 12 weeks. Anthropometric measurements and blood samples were collected at baseline and after 12 weeks. Analysis of covariance was used to compare changes between the groups with pre-test values as covariates.
After 12 weeks, asprosin concentration (p = 0.04), resistance insulin (p = 0.01), HbA1c (p = 0.001), fasting glucose (p = 0.01), weight (p = 0.04), body mass index (0.03), body fat percentage (0.001), total cholesterol (p = 0.04), Triglycerides (p = 0.03) significantly decreased in the exercise group compared to the control group. Significant changes were not observed at the complement C1q tumor necrosis factor-related protein-1 concentration (p = 0.52), WHR (p = 0.29), high-density lipoprotein cholesterol (p = 0.85), low-density lipoprotein cholesterol (p = 0.12), and fat free mass (p = 0.98) between the groups. After 12 weeks in the combined exercise group, low-density lipoprotein cholesterol (p = 0.007) decreased and high-density lipoprotein cholesterol (p = 0.01) increased significantly compared with the baseline. There was a significant positive correlation between asprosin changes with HbA1c(r = 0.44, p = 0.02) and resistance insulin (r = 0.43, p = 0.03) changes.
These findings suggest that combined resistance aerobic exercise training effectively decreases asprosin concentration and improves glycemic control in male patients with type 2 diabetes; however, it has no effect on complement C1q tumor necrosis factor-related protein-1 concentrations.
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The data and materials used and/or analyzed in the current study are available from the corresponding author, upon reasonable request.
Association AD (2018) Classification and diagnosis of diabetes: standards of medical care in diabetes—2018. Diabetes Care 41 (Supplement 1):S13-S27
Liang W, dong Ye D (2019) The potential of adipokines as biomarkers and therapeutic agents for vascular complications in type 2 diabetes mellitus. Cytokine Growth Factor Rev
Colberg SR, Sigal RJ, Fernhall B, Regensteiner JG, Blissmer BJ, Rubin RR, Chasan-Taber L, Albright AL, Braun B (2010) Exercise and type 2 diabetes: the American College of Sports Medicine and the American Diabetes Association: joint position statement. Diabetes Care 33(12):e147–e167
Stefanyk LE, Dyck DJ (2010) The interaction between adipokines, diet and exercise on muscle insulin sensitivity. Curr Opin Clin Nutr Metab Care 13(3):255–259
Berggren JR, Hulver MW, Houmard JA (2005) Fat as an endocrine organ: influence of exercise. J Appl Physiol 99(2):757–764
Han S, Kim JD, Lee S, Jeong AL, Park JS, Yong HJ, Boldbaatar A, Ka HI, Rhee E-J, Lee W-Y (2016) Circulating CTRP1 levels in type 2 diabetes and their association with FGF21. Int J Endocrinol. 2016
Romere C, Duerrschmid C, Bournat J, Constable P, Jain M, Xia F, Saha PK, Del Solar M, Zhu B, York B (2016) Asprosin, a fasting-induced glucogenic protein hormone. Cell 165(3):566–579
Zhang X, Jiang H, Ma X, Wu H (2019) Increased serum level and impaired response to glucose fluctuation of asprosin is associated with type 2 diabetes mellitus. J Diabetes Invest
Duerrschmid C, He Y, Wang C, Li C, Bournat JC, Romere C, Saha PK, Lee ME, Phillips KJ, Jain M (2017) Asprosin is a centrally acting orexigenic hormone. Nat Med 23(12):1444
Hosseini ZS, Heydari-Zarnagh H, Behmanesh M, Miri M (2020) Maternal exposure to air pollution and umbilical asprosin concentration, a novel insulin-resistant marker. Chemosphere:129228
Wang Y, Qu H, Xiong X, Qiu Y, Liao Y, Chen Y, Zheng Y, Zheng H (2018) Plasma asprosin concentrations are increased in individuals with glucose dysregulation and correlated with insulin resistance and first-phase insulin secretion. Med Inflam 2018
Li X, Liao M, Shen R, Zhang L, Hu H, Wu J, Wang X, Qu H, Guo S, Long M (2018) Plasma asprosin levels are associated with glucose metabolism, lipid, and sex hormone profiles in females with metabolic-related diseases. Med Inflam 2018
Yuan M, Li W, Zhu Y, Yu B, Wu J (2020) Asprosin: a novel player in metabolic diseases. Front Endocrinol 11:64
Zhang L, Chen C, Zhou N, Fu Y, Cheng X (2019) Circulating asprosin concentrations are increased in type 2 diabetes mellitus and independently associated with fasting glucose and triglyceride. Clin Chim Acta 489:183–188
Naiemian S, Naeemipour M, Zarei M, Najafi ML, Gohari A, Behroozikhah MR, Heydari H, Miri M (2020) Serum concentration of asprosin in new-onset type 2 diabetes. Diabetol Metab Synd 12(1):1–8
Xin Y, Lyu X, Wang C, Fu Y, Zhang S, Tian C, Li Q, Zhang D (2014) Elevated circulating levels of CTRP1, a novel adipokine, in diabetic patients. Endocr J 61(9):841–847
Xin Y, Zhang D, Fu Y, Wang C, Li Q, Tian C, Zhang S, Lyu X (2017) C1qtnf-related protein 1 improve insulin resistance by reducing phosphorylation of serine 1101 in insulin receptor substrate 1. Endocr J:EJ17–0128
Peterson JM, Aja S, Wei Z, Wong GW (2012) CTRP1 protein enhances fatty acid oxidation via AMP-activated protein kinase (AMPK) activation and acetyl-CoA carboxylase (ACC) inhibition. J Biol Chem 287(2):1576–1587
Seldin MM, Tan SY, Wong GW (2014) Metabolic function of the CTRP family of hormones. Rev Endoc Metab Dis 15(2):111–123
Han S, Park JS, Lee S, Jeong AL, Oh KS, Ka HI, Choi H-J, Son W-C, Lee W-Y, Oh SJ (2016) CTRP1 protects against diet-induced hyperglycemia by enhancing glycolysis and fatty acid oxidation. J Nutri Biochem 27:43–52
Pan X, Lu T, Wu F, Jin L, Zhang Y, Shi L, Li X, Lin Z (2014) Circulating complement-C1q TNF-related protein 1 levels are increased in patients with type 2 diabetes and are associated with insulin sensitivity in Chinese subjects. PLoS One 9 (5)
Rodriguez S, Lei X, Petersen PS, Tan SY, Little HC, Wong GW (2016) Loss of CTRP1 disrupts glucose and lipid homeostasis. Am J Physiol Endocrinol Metab 311(4):E678–E697
Han S, Yang Y (2018) A novel blood pressure modulator C1q/TNF-α–related protein 1 (CTRP1). BMB Rep 51(12):611
Yagmur E, Buergerhausen D, Koek GH, Weiskirchen R, Trautwein C, Koch A, Tacke F (2019) Elevated CTRP1 plasma concentration is associated with sepsis and pre-existing type 2 diabetes mellitus in critically Ill patients. J Clin Med 8(5):661
Colberg SR, Sigal RJ, Yardley JE, Riddell MC, Dunstan DW, Dempsey PC, Horton ES, Castorino K, Tate DF (2016) Physical activity/exercise and diabetes: a position statement of the American Diabetes Association. Diabetes Care 39(11):2065–2079
Hansen D, Peeters S, Zwaenepoel B, Verleyen D, Wittebrood C, Timmerman N, Schotte M (2013) Exercise assessment and prescription in patients with type 2 diabetes in the private and home care setting: clinical recommendations from AXXON (Belgian Physical Therapy Association). Phys Ther 93(5):597–610
Hordern MD, Dunstan DW, Prins JB, Baker MK, Singh MAF, Coombes JS (2012) Exercise prescription for patients with type 2 diabetes and pre-diabetes: a position statement from Exercise and Sport Science Australia. J Sci Med Sport 15(1):25–31
Brzycki M (1993) Strength testing—predicting a one-rep max from reps-to-fatigue. J Phys Educ Recreat Dance 64(1):88–90
Sigal RJ, Armstrong MJ, Bacon SL, Boule NG, Dasgupta K, Kenny GP, Riddell MC (2018) Physical activity and diabetes. Can J Diabetes 42:S54–S63
AminiLari Z, Fararouei M, Amanat S, Sinaei E, Dianatinasab S, AminiLari M, Daneshi N, Dianatinasab M (2017) The effect of 12 weeks aerobic, resistance, and combined exercises on omentin-1 levels and insulin resistance among type 2 diabetic middle-aged women. Diabetes Metab J 41(3):205–212
Jorge MLMP, de Oliveira VN, Resende NM, Paraiso LF, Calixto A, Diniz ALD, Resende ES, Ropelle ER, Carvalheira JB, Espindola FS (2011) The effects of aerobic, resistance, and combined exercise on metabolic control, inflammatory markers, adipocytokines, and muscle insulin signaling in patients with type 2 diabetes mellitus. Metabolism 60(9):1244–1252
Church TS, Blair SN, Cocreham S, Johannsen N, Johnson W, Kramer K, Mikus CR, Myers V, Nauta M, Rodarte RQ (2010) Effects of aerobic and resistance training on hemoglobin A1c levels in patients with type 2 diabetes: a randomized controlled trial. JAMA 304(20):2253–2262
Greenhill C (2016) Liver: asprosin—new hormone involved in hepatic glucose release. Nat Rev Endocrinol 12(6):312
Ko JR, Seo DY, Kim TN, Park SH, Kwak H-B, Ko KS, Rhee BD, Han J (2019) Aerobic exercise training decreases hepatic asprosin in diabetic rats. J Clin Med 8(5):666
Nakhaei H, Mogharnasi M, Fanaei H (2019) Effect of swimming training on levels of asprosin, lipid profile, glucose and insulin resistance in rats with metabolic syndrome. Obes Med 15:100111
Kajimura S (2017) Advances in the understanding of adipose tissue biology. Nat Rev Endocrinol 13(2):69–70
Salminen A, Kaarniranta K, Kauppinen A (2016) Age-related changes in AMPK activation: role for AMPK phosphatases and inhibitory phosphorylation by upstream signaling pathways. Age Res Rev 28:15–26
Ferretti AC, Tonucci FM, Hidalgo F, Almada E, Larocca MC, Favre C (2016) AMPK and PKA interaction in the regulation of survival of liver cancer cells subjected to glucose starvation. Oncotarget 7(14):17815
Wang M, Yin C, Wang L, Liu Y, Li H, Li M, Yi X, Xiao Y (2019) Serum asprosin concentrations are increased and associated with insulin resistance in children with obesity. Ann Nutr Metab 75(4):205–212
Wiecek M, Szymura J, Maciejczyk M, Kantorowicz M, Szygula Z (2018) Acute anaerobic exercise affects the secretion of Asprosin, Irisin, and other cytokines–a comparison between sexes. Front Physiol 9:1782
Schumann U, Qiu S, Enders K, Bosnyák E, Laszlo R, Machus K, Trájer E, Jaganathan S, Zügel M, Steinacker J (2017) Asprosin, a newly identified fasting-induced hormone is not elevated in obesity and is insensitive to acute exercise: 3592 board #39 June 3 8. Med Sci Sports Exerc 49:1023. https://doi.org/10.1249/01.mss.0000519807.24061.ca
Schäffler A, Buechler C (2012) CTRP family: linking immunity to metabolism. Trends Endocrinol Metab 23(4):194–204
Shabani P, Emamgholipour S, Doosti M (2017) CTRP1 in liver disease. In: Advances in clinical chemistry, vol 79. Elsevier, pp 1–23
Kon M, Ebi Y, Nakagaki K (2019) Effects of a single bout of high-intensity interval exercise on C1q/TNF-related proteins. Appl Physiol Nutr Metab 44(1):47–51
Kim KY, Kim HY, Kim JH, Lee CH, Kim DH, Lee YH, Han SH, Lim JS, Cho DH, Lee MS, Yoon S, Kim KI, Yoon DY, Yang Y (2006) Tumor necrosis factor-alpha and interleukin-1beta increases CTRP1 expression in adipose tissue. FEBS Lett 580(16):3953–3960. https://doi.org/10.1016/j.febslet.2006.06.034
Kadoglou NP, Iliadis F, Angelopoulou N, Perrea D, Ampatzidis G, Liapis CD, Alevizos M (2007) The anti-inflammatory effects of exercise training in patients with type 2 diabetes mellitus. Eur J Cardiovascu Prevent Rehabilit 14(6):837–843
Melo L, Dativo-Medeiros J, Menezes-Silva C, Barbosa F, Sousa-Rodrigues C, Rabelo L (2017) Physical exercise on inflammatory markers in type 2 diabetes patients: a systematic review of randomized controlled trials. Oxidat Med Cell Long 2017:1–10. https://doi.org/10.1155/2017/8523728
Thanks are owed to the patients and volunteers participated in this study.
The present study was financially supported by University of Neyshabur, Iran.
Conflict of interest
The study authors declare no conflict of interest.
The study protocol was carried out in compliance with the Declaration of Helsinki and was approved by the Ethics Committee of Sabzevar University of Medical Sciences and registered number IRCT20181006041252N15 in Iranian Registry of Clinical Trials (Primary Registry in the WHO Registry Network).
Written informed consent was obtained from all volunteers.
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Zarei, M., Nakhzari Khodakheyr, J., Rashidlamir, A. et al. The effect of combined resistance aerobic exercise training on concentrations of asprosin and complement C1q tumor necrosis factor-related protein-1 in men with type 2 diabetes. Sport Sci Health (2021). https://doi.org/10.1007/s11332-021-00738-7
- Asprosin protein
- C1QTNF1 protein
- Type 2 diabetes