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Biochemical changes in oxidative stress markers following endurance training and consumption of purslane seed in rats with hydrogen peroxide-induced toxicity

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Abstract

The current study examined fluctuations in oxidative stress markers following endurance training (ET) and consumption of purslane seeds (Ps) in rats after receiving H2O2. Fifty-four adult male Wistar rats were assigned to nine experimental groups: (1) control (intoxicated-no treatment); (2) ET; (3) ET + Ps 50 mg/kg/day; (4) ET + Ps 200 mg/kg/day; (5) ET + Ps 400 mg/kg/day; (6) Ps 50 mg/kg/day; (7) Ps 200 mg/kg/day; (8) Ps 400 mg/kg/day; (9) control (non-intoxicated, intact). The first eight groups were given 100 mg/kg of H2O2 to induce oxidative stress. Groups 2–5 were given ET for a period of 8 weeks. Heart and lung tissues were then exposed to evaluate the oxidative stress markers. Catalase, glutathione peroxidase, malondialdehyde, and superoxide dismutase enzymes were measured using ELISA kits. A marked improvement in enzyme concentration was observed in both tissues. It was more pronounced in the groups receiving higher doses of Ps + ET. The findings provide evidence that purslane seed supplementation has antioxidant potential alongside endurance training and improved the ability to cope with oxidative stress.

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  1. Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Tehran, Tehran, Iran

    Rahman Soori, Valiollah Shahedi, Ali Akbarnejad & Siroos Choobineh

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  1. Rahman Soori
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Correspondence to Rahman Soori.

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Soori, R., Shahedi, V., Akbarnejad, A. et al. Biochemical changes in oxidative stress markers following endurance training and consumption of purslane seed in rats with hydrogen peroxide-induced toxicity. Sport Sci Health 15, 133–139 (2019). https://doi.org/10.1007/s11332-018-0501-y

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  • DOI: https://doi.org/10.1007/s11332-018-0501-y

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