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Long-term effects of sprint interval training on expression of cardiac genes involved in energy efficiency

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Abstract

Purpose

The real mechanisms of intensive exercise training-induced energy efficiency have not yet been well examined. Therefore, the aim of the present study was to investigate the effects of sprint interval training (SIT) on gene expression of uncoupling proteins (UCPs) and endothelial nitric oxide synthase (eNOS).

Methods

For this purpose, 16 Albino Wistar rats (250–300 g) were randomly divided into equal groups of control and sprint training. The animals run on treadmill for 10 weeks, 5 days per week at intensity corresponding to 90–95% maximal oxygen consumption. The gene expression of UCP2, UCP3 and eNOS was analyzed by RT-PCR method in hearts. The data were analyzed by independent samples T test at P < 0.05 level.

Results

Sprint interval training significantly decreased mRNA expression of UCP2 (t14 = 4.818, P = 0.001) and UCP3 (t14 = 4.620, P = 0.001) in cardiac muscle of rats. In contrast, mRNA expression of eNOS in cardiac muscle significantly increased following sprint interval training (t14 = 7.967, P = 0.001).

Conclusion

This study elucidates that SIT through reduction in gene expression of uncoupling proteins can improve energy efficiency. But, more studies are needed to confirm this hypothesis.

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Abbreviations

C:

Control

eNOS:

Endothelial nitric oxide synthase

SIT:

Sprint interval training

UCPs:

Uncoupling proteins

VO2max:

Maximal oxygen uptake

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Acknowledgements

We thank the staff of the animal laboratory at Baqiyatallah University of Medical Sciences for their valuable assistance with us in carrying out the exercise protocols and animal surgery.

Funding

This research received no specific grant from any funding agency in the public, commercial, or not-for-profit sectors.

Author information

Authors and Affiliations

  1. Department of Sport Sciences, University of Bojnord, Bojnord, Iran

    Hossein TaheriChadorneshin

  2. Department of Biology, College of Basic Sciences, Yadegar-e-Imam Khomeini (RAH) Shahre Rey Branch, Islamic Azad University, Tehran, Iran

    Fatemeh Rostamkhani

  3. Exercise Physiology Research Center, Life Style Institute, Baqiyatallah University of Medical Sciences, Nosrati alley, sheikh Bahaei Street, Mollasadra Street, Vanak Square, Tehran, Post Office Box 19395-5487, Iran

    Hossein Shirvani

Authors
  1. Hossein TaheriChadorneshin
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Correspondence to Hossein Shirvani.

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The authors declare that they have no conflict of interest.

Ethical approval

All methods performed in the study were in accordance with ethical standards of the national research committee and with the 1964 Helsinki Declaration.

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There is no informed consent for this study since the study was not conducted on humans.

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TaheriChadorneshin, H., Rostamkhani, F. & Shirvani, H. Long-term effects of sprint interval training on expression of cardiac genes involved in energy efficiency. Sport Sci Health 15, 59–63 (2019). https://doi.org/10.1007/s11332-018-0480-z

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  • DOI: https://doi.org/10.1007/s11332-018-0480-z

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