Sport Sciences for Health

, Volume 14, Issue 2, pp 387–392 | Cite as

Effect of carbohydrates versus carbohydrates plus proteins and antioxidants on oxidative stress and muscle damage induced by single bout resistance exercise

  • Daniel dos Santos Ferreira
  • Lydiane Tavares Toscano
  • Tayse Guedes Cabral
  • Gilberto Santos Cerqueira
  • Ana Carla Lima de França
  • Alexandre Sérgio Silva
Original Article


Although nutritional supplementation is controversial in the literature, the ergogenic value attributed to carbohydrate supplementation is recognized by delaying fatigue in resistance exercises. The aim of this study was to investigate the effect of supplementation of a commercial carbohydrate compound plus protein and antioxidants (CPA) in muscle damage and oxidative stress induced by a single resistance training session. Ten healthy young subjects (24 ± 4 years; 23.2 ± 1 kg/m2; \( V{\text{O}}_{2\hbox{max} } \) 44.9 ± 10 ml/kg/min) performed three series of ten exercises until concentric failure, randomly ingesting water (WAT), isolated carbohydrates (CHO) or carbohydrates associated with proteins and antioxidants (CPA). Blood samples were taken before, immediately and 24 h after each exercise session for analysis of muscle damage markers, creatine kinase (CK) and malondialdehyde (MDA) oxidative stress. Blood glucose was measured before, during and after the end of the exercises. CHO or CPA resulted in significant increases in glucose of 24 and 9.5%, respectively, at post-exercise compared to pre-exercise values. WAT resulted in a significant post-exercise increase in CK (107.6 ± 34.9–227.4 ± 82.2 U/l; p = 0.02), while CHO promoted no significant increase (226 ± 90–318 ± 190 U/l; p = 0.02) and CPA did not promote a significant reduction (130 ± 125–121 ± 83 U/l). CHO or CPA did not affect the concentration of lipid peroxidation. This study revealed that ingestion of CHO or CPA during resistance exercise decreases muscle damage, but does not influence the lipidic peroxidation marker MDA.


Oxidative stress Carbohydrates Proteins Resistance exercise 


Compliance with ethical standards

Conflict of interest

The authors declare that they have no competing interests.

Ethical approval

The study was approved by the ethics committee and they also state that the research reported in the paper was undertaken in compliance with the Helsinki Declaration and the International Principles governing research on animals.

Informed consent

Informed consent was obtained from all participants included in the study.


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Copyright information

© Springer-Verlag Italia S.r.l., part of Springer Nature 2018

Authors and Affiliations

  • Daniel dos Santos Ferreira
    • 1
  • Lydiane Tavares Toscano
    • 1
  • Tayse Guedes Cabral
    • 1
  • Gilberto Santos Cerqueira
    • 2
  • Ana Carla Lima de França
    • 3
  • Alexandre Sérgio Silva
    • 1
  1. 1.Physical Education, Laboratory of Physical Training Applied to Performance and Health, Department of Physical EducationFederal University of Paraíba (UFPB), Health Sciences Center (DEF)João PessoaBrazil
  2. 2.Post-graduation Program in Morphofunctional Sciences of the Federal University of CearaFortalezaBrazil
  3. 3.Physiotherapy, Laboratory of Physical Training Applied to Performance and Health, Department of Physical EducationFederal University of ParaibaJoao PessoaBrazil

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