Testosterone and cortisol response to acute intermittent and continuous aerobic exercise in sedentary men
Different types of physical activity can induce different hormonal and physiological responses. In this study, we examined the testosterone, cortisol, creatine kinase (CK) and lactate dehydrogenase (LDH) response to acute intermittent (IE) and continuous (CE) aerobic exercise in sedentary men.
In this single-blinded randomised crossover study, eleven sedentary healthy males completed protocols (CE and IE) on two different days separated by a 1-week washout period. CE comprised 40 min of running on a treadmill at 60% of reserve heart rate. IE consisted of 40 min of running on a treadmill with intensity alternating between 50% (2 min) and 80% (1 min) of reserve heart rate. Blood samples were taken before and immediately after each exercise session.
Serum testosterone concentrations increased significantly after IE (+8.0%, P = 0.021) and decreased non-significantly after CE (−5.8%, P = 0.409). The IE response was greater than the CE response (P = 0.01). Cortisol concentration decreased in both IE and CE exercise (P = 0.001 and P = 0.016, respectively), by −33.6 and −34.6%, respectively. The testosterone to cortisol ratio increased significantly after both forms of exercise (IE: P = 0.003; CE: P = 0.041). CK concentrations significantly increased from PRE to POST (IE: +20.6%, P = 0.001; CE: +26.5%, P = 0.046). Despite the increase in concentrations of LDH, the changes were not significant (F (3, 30) = 1.01, P = 0.402; IE: +11.4% and CE: +23.1%).
In summary, it seems that intermittent exercise can be more useful in the development of body anabolic processes in sedentary men due to pronounced increases in testosterone.
KeywordsTestosterone Cortisol Testosterone to cortisol ratio Intermittent exercise Continuous exercise
The authors would like to thank the subjects for their committed participation.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflicts of interest.
All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.
Informed consent was obtained from all individual participants included in the study.
- 8.Esposito A, Bianchi V (2012) Cortisol: physiology, regulation and health implications. Nova Science Publishers Inc, New YorkGoogle Scholar
- 16.Molmen-Hansen HE, Stolen T, Tjonna AE, Aamot IL, Ekeberg IS, Tyldum GA, Wisloff U, Ingul CB, Stoylen A (2012) Aerobic interval training reduces blood pressure and improves myocardial function in hypertensive patients. Eur J Prev Cardiol 19(2):151–160. doi: 10.1177/1741826711400512 CrossRefPubMedGoogle Scholar
- 17.McArdle WD, Katch FI, Katch VL (2010) Exercise physiology: nutrition, energy, and human performance, 7th edn. Lippincott Williams & Wilkins, PhiladelphiaGoogle Scholar
- 21.Sgrò P, Romanelli F, Felici F, Sansone M, Bianchini S, Buzzachera C, Baldari C, Guidetti L, Pigozzi F, Lenzi A (2014) Testosterone responses to standardized short-term sub-maximal and maximal endurance exercises: issues on the dynamic adaptive role of the hypothalamic-pituitary-testicular axis. J Endocrinol Invest 37(1):13–24CrossRefPubMedGoogle Scholar
- 22.Vingren JL, Budnar RG, McKenzie AL, Duplanty AA, Luk H-Y, Levitt DE, Armstrong LE (2016) The acute testosterone, growth hormone, cortisol and interleukin-6 response to 164-km road cycling in a hot environment. J Sports Sci 34(8):694–699. doi: 10.1080/02640414.2015.1068440 CrossRefPubMedGoogle Scholar
- 25.Rodrigues P, Wassmansdorf R, Salgueirosa FM, Hernandez SG, Nascimento VB, Daros LB, Wharton L, Osiecki R (2016) Time-course of changes in indirect markers of muscle damage responses following a 130-km cycling race. Rev Brasileira de Cineantropometria Desempenho Humano 18(3):322–331CrossRefGoogle Scholar
- 29.Bally L, Zueger T, Buehler T, Dokumaci AS, Speck C, Pasi N, Ciller C, Paganini D, Feller K, Loher H (2016) Metabolic and hormonal response to intermittent high-intensity and continuous moderate intensity exercise in individuals with type 1 diabetes: a randomised crossover study. Diabetologia 59(4):776–784CrossRefPubMedGoogle Scholar