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Imaging Pulmonary Foreign Body Reaction Using [125I]iodo-DPA-713 SPECT/CT in Mice

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Abstract

Purpose

Foreign body reactions elicit granulomatous inflammation composed of reactive macrophages. We hypothesized that [125I]iodo-DPA-713 single-photon emission computed tomography (SPECT), a low-molecular-weight pyrazolopyrimidine ligand selectively trapped by phagocytes, could be used to detect foreign body reactions in a murine model.

Procedures

C57BL/6 mice intratracheally inoculated with dextran beads, which developed foreign body lesions, were imaged after injection of [125I]iodo-DPA-713 or DPA-713-IRDye800CW using SPECT and optical imaging, respectively.

Results

Foreign body lesions were clearly observed in the lungs of the dextran-treated mice on computer tomography imaging and demonstrated significantly higher [125I]iodo-DPA-713 uptake compared with control animals (p < 0.01). Ex vivo studies demonstrated granulomatous reactions in the lungs of dextran-treated mice and localization of DPA-713-IRDye800CW at the diseased sites confirming the imaging findings.

Conclusion

Radioiodinated DPA-713 may be used as a noninvasive biomarker for the detection of pulmonary foreign body reactions.

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References

  1. Rupprecht R, Papadopoulos V, Rammes G, Baghai TC, Fan J, Akula N, Groyer G, Adams D, Schumacher M (2010) Translocator protein (18 kDa) (TSPO) as a therapeutic target for neurological and psychiatric disorders. Nat Rev Drug Discov 9:971–988

    Article  CAS  PubMed  Google Scholar 

  2. Chen MK, Guilarte TR (2008) Translocator protein 18 kDa (TSPO): molecular sensor of brain injury and repair. Pharmacol Ther 118:1–17

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Foss CA, Harper JS, Wang H, Pomper MG, Jain SK (2013) Noninvasive molecular imaging of tuberculosis-associated inflammation with radioiodinated DPA-713. J Infect Dis 208:2067–2074

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Ordonez AA, Pokkali S, DeMarco VP et al (2015) Radioiodinated DPA-713 imaging correlates with bactericidal activity of tuberculosis treatments in mice. Antimicrob Agents Chemother 59:642–649

    Article  CAS  PubMed  Google Scholar 

  5. Anderson JM, Rodriguez A, Chang DT (2008) Foreign body reaction to biomaterials. Semin Immunol 20:86–100

    Article  CAS  PubMed  Google Scholar 

  6. Wang H, Pullambhatla M, Guilarte TR, Mease RC, Pomper MG (2009) Synthesis of [125I]iodoDPA-713: a new probe for imaging inflammation. Biochem Biophys Res Commun 389:80–83

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. Foss CA, Liu L, Mease RC, Wang H, Pasricha P, Pomper MG (2017) Imaging macrophage accumulation in a murine model of chronic pancreatitis with 125I-iodo-DPA-713 SPECT/CT. J Nucl Med 58:1685–1690

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. Foss CA, Bedja D, Mease RC, Wang H, Kass DA, Chatterjee S, Pomper MG (2015) Molecular imaging of inflammation in the ApoE -/- mouse model of atherosclerosis with iodoDPA. Biochem Biophys Res Commun 461:70–75

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Tucker EW, Pokkali S, Zhang Z, DeMarco VP, Klunk M, Smith ES, Ordonez AA, Penet MF, Bhujwalla Z, Jain SK, Kannan S (2016) Microglia activation in a pediatric rabbit model of tuberculous meningitis. Dis Model Mech 9:1497–1506

    Article  PubMed  PubMed Central  Google Scholar 

  10. Bendeck SE, Leung AN, Berry GJ, Daniel D, Ruoss SJ (2001) Cellulose granulomatosis presenting as centrilobular nodules: CT and histologic findings. AJR Am J Roentgenol 177:1151–1153

    Article  CAS  PubMed  Google Scholar 

  11. Radow SK, Nachamkin I, Morrow C, Fairman RP, Fratkin M, Rodriguez GE, Glauser FL (1983) Foreign body granulomatosis. Clinical and immunologic findings. Am Rev Respir Dis 127:575–580

    Article  CAS  PubMed  Google Scholar 

  12. James ML, Fulton RR, Henderson DJ, Eberl S, Meikle SR, Thomson S, Allan RD, Dolle F, Fulham MJ, Kassiou M (2005) Synthesis and in vivo evaluation of a novel peripheral benzodiazepine receptor PET radioligand. Bioorg Med Chem 13:6188–6194

    Article  CAS  PubMed  Google Scholar 

  13. Foss CA, Plyku D, Ordonez AA, Sanchez-Bautista J, Rosenthal HB, Minn IL, Lodge MA, Pomper MG, Sgouros G, Jain SK (2018) Biodistribution and radiation dosimetry of (124)I-iodo-DPA-713, a PET radiotracer for macrophage-associated inflammation. J Nucl Med:jnumed.117.207431

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Funding

This study was funded by the National Institutes of Health (NIH) Director’s Transformative Research Award R01-EB020539 (S.K.J.). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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Authors and Affiliations

  1. Center for Infection and Inflammation Imaging Research, Johns Hopkins University, 1550 Orleans Street, CRB-II, Rm 1.09, Baltimore, MD, 21287, USA

    Julian Sanchez-Bautista, Catherine A. Foss, Alvaro A. Ordonez, Mariah H. Klunk & Sanjay K. Jain

  2. Center for Tuberculosis Research, Johns Hopkins University, Baltimore, MD, USA

    Julian Sanchez-Bautista, Alvaro A. Ordonez, Mariah H. Klunk & Sanjay K. Jain

  3. Department of Pediatrics, Johns Hopkins University, Baltimore, MD, USA

    Julian Sanchez-Bautista, Alvaro A. Ordonez, Mariah H. Klunk & Sanjay K. Jain

  4. Russell H. Morgan Department of Radiology and Radiological Sciences, Johns Hopkins University, Baltimore, MD, USA

    Catherine A. Foss & Sanjay K. Jain

Authors
  1. Julian Sanchez-Bautista
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  2. Catherine A. Foss
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  3. Alvaro A. Ordonez
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  4. Mariah H. Klunk
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  5. Sanjay K. Jain
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Corresponding author

Correspondence to Sanjay K. Jain.

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Conflict of Interest

The authors declare that they have no conflict of interest.

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Sanchez-Bautista, J., Foss, C.A., Ordonez, A.A. et al. Imaging Pulmonary Foreign Body Reaction Using [125I]iodo-DPA-713 SPECT/CT in Mice. Mol Imaging Biol 21, 228–231 (2019). https://doi.org/10.1007/s11307-018-1249-0

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  • DOI: https://doi.org/10.1007/s11307-018-1249-0

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