Molecular Imaging and Biology

, Volume 21, Issue 2, pp 232–239 | Cite as

In Vivo Mapping and Quantification of Creatine Using Chemical Exchange Saturation Transfer Imaging in Rat Models of Epileptic Seizure

  • Dong-Hoon Lee
  • Do-Wan Lee
  • Jae-Im Kwon
  • Chul-Woong Woo
  • Sang-Tae Kim
  • Jin Seong Lee
  • Choong Gon Choi
  • Kyung Won Kim
  • Jeong Kon Kim
  • Dong-Cheol WooEmail author
Research Article



To evaluate signal changes in the hippocampus of epileptic seizure rat models, based on quantified creatine chemical exchange saturation transfer (CrCEST) signals.


CEST data and 1H magnetic resonance spectroscopy (1H MRS) data were obtained for the two imaging groups: control (CTRL) and epileptic seizure-induced (ES; via kainic acid [KA] injection) groups. CrCEST signals in the hippocampal regions were quantitatively evaluated; correlations between CrCEST signals and phosphocreatine (PCr) and total creatine (tCr; PCr + Cr) concentrations, derived from the analysis of 1H MRS data, were investigated as a function of time changes (before KA injection, 3 and 5 h after KA injection).


Measured CrCEST signals were exhibited significant differences between before and after KA injection in the ES group. At each time point, CrCEST signals showed significant correlations with PCr concentration (all |r| > 0.59; all P < 0.05); no significant correlations were found between CrCEST signals and tCr concentrations (all |r| < 0.22; all P > 0.05).


CrCEST can adequately detect changes in the concentration of Cr as a result of energy metabolism, and may serve as a potentially useful tool for diagnosis and assessment of prognosis in epilepsy.

Key words

Creatine Chemical exchange saturation transfer Phosphocreatine Epilepsy Magnetic resonance spectroscopy 


Funding Information

This study was supported by grants of Basic Science Research Program through the National Research Foundation of Korea funded by the Ministry of Education [NRF ( NRF-2017R1A6A3A03012461 and NRF-2018R1A2B2007694] and the Korea Health Technology R&D Project through the Korea Health Industry Development Institute [KHIDI ( HI14C1090], funded by the Ministry of Health & Welfare, Republic of Korea.

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.


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Copyright information

© World Molecular Imaging Society 2018

Authors and Affiliations

  1. 1.Faculty of Health Sciences and Brain & Mind CentreThe University of SydneySydneyAustralia
  2. 2.Center for Bioimaging of New Drug Development, and MR Core Laboratory, Asan Medical CenterAsan Institute for Life SciencesSeoulRepublic of Korea
  3. 3.MR Core Laboratory, Asan Medical CenterAsan Institute for Life SciencesSeoulRepublic of Korea
  4. 4.Department of Radiology, Asan Medical CenterUniversity of Ulsan College of MedicineSeoulRepublic of Korea
  5. 5.Department of Convergence Medicine, Asan Medical CenterUniversity of Ulsan College of MedicineSeoulRepublic of Korea

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