MHI-148 Cyanine Dye Conjugated Chitosan Nanomicelle with NIR Light-Trigger Release Property as Cancer Targeting Theranostic Agent

  • Reju George Thomas
  • Myeong Ju Moon
  • Suchithra Poilil Surendran
  • Hyeong Ju Park
  • In-Kyu Park
  • Byeong-Il Lee
  • Yong Yeon Jeong
Research Article
  • 16 Downloads

Abstract

Purpose

Paclitaxel (PTX) loaded hydrophobically modified glycol chitosan (HGC) micelle is biocompatible in nature, but it requires cancer targeting ability and stimuli release property for better efficiency. To improve tumor retention and drug release characteristic of HGC-PTX nanomicelles, we conjugated cancer targeting heptamethine dye, MHI-148, which acts as an optical imaging agent, targeting moiety and also trigger on-demand drug release on application of NIR 808 nm laser.

Procedures

The amine group of glycol chitosan modified with hydrophobic 5β-cholanic acid and the carboxyl group of MHI-148 were bonded by 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide/N-hydroxysuccinimide chemistry. Paclitaxel was loaded to MHI-HGC nanomicelle by an oil-in-water emulsion method, thereby forming MHI-HGC-PTX.

Results

Comparison of near infrared (NIR) dyes, MHI-148, and Flamma-774 conjugated to HGC showed higher accumulation for MHI-HGC in 4T1 tumor and 4T1 tumor spheroid. In vitro studies showed high accumulation of MHI-HGC-PTX in 4T1 and SCC7 cancer cell lines compared to NIH3T3 cell line. In vivo fluorescence imaging of the 4T1 and SCC7 tumor showed peak accumulation of MHI-HGC-PTX at day 1 and elimination from the body at day 6. MHI-HGC-PTX showed good photothermal heating ability (50.3 °C), even at a low concentration of 33 μg/ml in 1 W/cm2 808 nm laser at 1 min time point. Tumor reduction studies in BALB/c nude mice with SCC7 tumor showed marked reduction in MHI-HGC-PTX in the PTT group combined with photothermal therapy compared to MHI-HGC-PTX in the group without PTT.

Conclusion

MHI-HGC-PTX is a cancer theranostic agent with cancer targeting and optical imaging capability. Our studies also showed that it has cancer targeting property independent of tumor type and tumor reduction property by combined photothermal and chemotherapeutic effects.

Key words

Cancer Photothermal therapy Heptamethine Trigger release 

Notes

Acknowledgements

This research was supported by Basic Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science and Future Planning (2015R1A2A2A01007798).

Compliance with Ethical Standards

The animal experiment was conducted in agreement with National Institutes of Health guide for the care and use of Laboratory animals and approved by Chonnam National University Medical School Research Institutional Animal Care and Use Committee (CNUHH 2014-148).

Conflict of Interest

The authors declare that they have no conflict of interest.

Supplementary material

11307_2018_1169_MOESM1_ESM.pdf (505 kb)
ESM 1 (PDF 505 kb)

References

  1. 1.
    Hervault A, Thanh NT (2014) Magnetic nanoparticle-based therapeutic agents for thermo-chemotherapy treatment of cancer. Nano 6:11553–11573Google Scholar
  2. 2.
    John JV, Thomas RG, Lee HR, Chen H, Jeong YY, Kim I (2016) Phospholipid end-capped acid-degradable polyurethane micelles for intracellular delivery of cancer therapeutics. Adv Healthcare Mater 5:1874–1883CrossRefGoogle Scholar
  3. 3.
    Ramachandra Kurup Sasikala A, Thomas RG, Unnithan AR, Saravanakumar B, Jeong YY, Park CH, Kim CS (2016) Multifunctional Nanocarpets for cancer Theranostics: remotely controlled graphene Nanoheaters for Thermo-Chemosensitisation and magnetic resonance imaging. Sci Rep 6:20543CrossRefPubMedPubMedCentralGoogle Scholar
  4. 4.
    Yue C, Zhang C, Alfranca G, Yang Y, Jiang X, Yang Y, Pan F, Fuente JM et al (2016) Near-infrared light triggered ROS-activated Theranostic platform based on Ce6-CPT-UCNPs for simultaneous fluorescence imaging and chemo-photodynamic combined therapy. Theranostics 6:456–469CrossRefPubMedPubMedCentralGoogle Scholar
  5. 5.
    Kwon S, Park JH, Chung H, Kwon IC, Jeong SY, Kim IS (2003) Physicochemical characteristics of self-assembled nanoparticles based on glycol chitosan bearing 5β-Cholanic acid. Langmuir 19:10188–10193CrossRefGoogle Scholar
  6. 6.
    Kim J-H, Kim Y-S, Kim S, Park JH, Kim K, Choi K, Chung H, Jeong SY, Park RW, Kim IS, Kwon IC (2006) Hydrophobically modified glycol chitosan nanoparticles as carriers for paclitaxel. J Control Release 111:228–234CrossRefPubMedGoogle Scholar
  7. 7.
    Key J, Cooper C, Kim AY, Dhawan D, Knapp DW, Kim K, Park JH, Choi K, Kwon IC, Park K, Leary JF (2012) In vivo NIRF and MR dual-modality imaging using glycol chitosan nanoparticles. J Control Release 163:249–255CrossRefPubMedGoogle Scholar
  8. 8.
    Guo F, Yu M, Wang J, Tan F, Li N (2015) Smart IR780 Theranostic Nanocarrier for tumor-specific therapy: hyperthermia-mediated bubble-generating and folate-targeted liposomes. ACS Appl Mater Interfaces 7:20556–20567CrossRefPubMedGoogle Scholar
  9. 9.
    Wang H, Agarwal P, Zhao S, Yu J, Lu X, He X (2015) A biomimetic hybrid nanoplatform for encapsulation and precisely controlled delivery of theranostic agents. [corrected]. Nat Commun 6:10081CrossRefPubMedPubMedCentralGoogle Scholar
  10. 10.
    GhavamiNejad A, SamariKhalaj M, Aguilar LE, Park CH, Kim CS (2016) pH/NIR light-controlled multidrug release via a mussel-inspired nanocomposite hydrogel for chemo-Photothermal cancer therapy. Sci Rep 6:33594CrossRefPubMedPubMedCentralGoogle Scholar
  11. 11.
    Wang T, Wang D, Yu H, Wang M, Liu J, Feng B, Zhou F, Yin Q, Zhang Z, Huang Y, Li Y (2016) Intracellularly acid-switchable multifunctional micelles for combinational photo/chemotherapy of the drug-resistant tumor. ACS Nano 10:3496–3508CrossRefPubMedGoogle Scholar
  12. 12.
    Wang X, Zhang J, Wang Y, Wang C, Xiao J, Zhang Q, Cheng Y (2016) Multi-responsive photothermal-chemotherapy with drug-loaded melanin-like nanoparticles for synergetic tumor ablation. Biomaterials 81:114–124CrossRefPubMedGoogle Scholar
  13. 13.
    Kaur P, Aliru ML, Chadha AS, Asea A, Krishnan S (2016) Hyperthermia using nanoparticles – promises and pitfalls. Int J Hyperth 32:76–88CrossRefGoogle Scholar
  14. 14.
    Yi X, Wang F, Qin W, Yang X, Yuan J (2014) Near-infrared fluorescent probes in cancer imaging and therapy: an emerging field. Int J Nanomedicine 9:1347–1365CrossRefPubMedPubMedCentralGoogle Scholar
  15. 15.
    Zhang E, Zhang C, Su Y, Cheng T, Shi C (2011) Newly developed strategies for multifunctional mitochondria-targeted agents in cancer therapy. Drug Discov Today 16:140–146CrossRefPubMedGoogle Scholar
  16. 16.
    Lee S, George Thomas R, Ju Moon M, Ju Park H, Park IK, Lee BI, Yeon Jeong Y (2017) Near-infrared Heptamethine cyanine based iron oxide nanoparticles for tumor targeted multimodal imaging and Photothermal therapy. Sci Rep 7:2108CrossRefPubMedPubMedCentralGoogle Scholar
  17. 17.
    Yeh CS, Su CH, Ho WY, Huang CC, Chang JC, Chien YH, Hung ST, Liau MC, Ho HY (2013) Tumor targeting and MR imaging with lipophilic cyanine-mediated near-infrared responsive porous Gd silicate nanoparticles. Biomaterials 34:5677–5688CrossRefPubMedGoogle Scholar
  18. 18.
    Xiao L, Zhang Y, Yue W, Xie X, Wang JP, Chordia MD, Chung LWK, Pan D (2013) Heptamethine cyanine based 64Cu-PET probe PC-1001 for cancer imaging: synthesis and in vivo evaluation. Nucl Med Biol 40:351–360CrossRefPubMedPubMedCentralGoogle Scholar
  19. 19.
    Weiswald LB, Bellet D, Dangles-Marie V (2015) Spherical cancer models in tumor biology. Neoplasia 17:1–15CrossRefPubMedPubMedCentralGoogle Scholar
  20. 20.
    Wang Y, Liu T, Zhang E, Luo S, Tan X, Shi C (2014) Preferential accumulation of the near infrared heptamethine dye IR-780 in the mitochondria of drug-resistant lung cancer cells. Biomaterials 35:4116–4124CrossRefPubMedGoogle Scholar
  21. 21.
    Buxhofer-Ausch V, Secky L, Wlcek K et al (2013) Tumor-specific expression of organic anion-transporting polypeptides: transporters as novel targets for cancer therapy. J Drug Delivery 2013:863539CrossRefGoogle Scholar
  22. 22.
    Wu JB, Shao C, Li X, Shi C, Li Q, Hu P, Chen YT, Dou X, Sahu D, Li W, Harada H, Zhang Y, Wang R, Zhau HE, Chung LWK (2014) Near-infrared fluorescence imaging of cancer mediated by tumor hypoxia and HIF1alpha/OATPs signaling axis. Biomaterials 35:8175–8185CrossRefPubMedPubMedCentralGoogle Scholar
  23. 23.
    Zhang C, Liu T, Su Y, Luo S, Zhu Y, Tan X, Fan S, Zhang L, Zhou Y, Cheng T, Shi C (2010) A near-infrared fluorescent heptamethine indocyanine dye with preferential tumor accumulation for in vivo imaging. Biomaterials 31:6612–6617CrossRefPubMedGoogle Scholar
  24. 24.
    Zhang E, Luo S, Tan X, Shi C (2014) Mechanistic study of IR-780 dye as a potential tumor targeting and drug delivery agent. Biomaterials 35:771–778CrossRefPubMedGoogle Scholar
  25. 25.
    Dong Z, Gong H, Gao M, Zhu W, Sun X, Feng L, Fu T, Li Y, Liu Z (2016) Polydopamine nanoparticles as a versatile molecular loading platform to enable imaging-guided cancer combination therapy. Theranostics 6:1031–1042CrossRefPubMedPubMedCentralGoogle Scholar
  26. 26.
    Kumar P, Srivastava R (2015) IR 820 stabilized multifunctional polycaprolactone glycol chitosan composite nanoparticles for cancer therapy. RSC Adv 5:56162–56170CrossRefGoogle Scholar

Copyright information

© World Molecular Imaging Society 2018

Authors and Affiliations

  • Reju George Thomas
    • 1
    • 2
  • Myeong Ju Moon
    • 1
    • 2
  • Suchithra Poilil Surendran
    • 1
    • 2
  • Hyeong Ju Park
    • 3
  • In-Kyu Park
    • 4
  • Byeong-Il Lee
    • 3
  • Yong Yeon Jeong
    • 1
    • 2
  1. 1.Department of RadiologyChonnam National University Hwasun HospitalHwasunSouth Korea
  2. 2.Biomolecular Theranostics (BiT) LabGwangjuSouth Korea
  3. 3.Medical Photonics Research CenterKorea Photonics Technology InstituteGwangjuSouth Korea
  4. 4.Department of Biomedical SciencesChonnam National University Medical SchoolGwangjuSouth Korea

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