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Breast Cancer Cells Imaging By Targeting Methionine Transporters with Gadolinium-Based Nanoprobe

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Molecular Imaging and Biology Aims and scope Submit manuscript

Abstract

Purpose

Early cancer diagnosis using MRI imaging is of high global interest as a non-invasive and powerful modality. In this study, methionine was conjugated on gadolinium-based mesoporous silica nanospheres to evaluate intra-cellular uptake and its accumulation in human breast cancer cells.

Procedures

The contrast agent was synthesized and characterized using different techniques including N2 physisorption, thermal gravimetric analysis, dynamic light scattering, and inductively coupled plasma atomic emission spectroscopy (ICP-AES). The intra-cellular uptake of Gd3+ was measured by ICP-AES, fluorescent microscopy, and flow cytometry. Finally, cellular and tumor MR imaging were performed to determine in vitro and in vivo relaxometry.

Results

According to the results, the contrast agents accumulated in tumor cells both in vitro and in vivo. There was no significant cellular toxicity on either normal or cancer cells along with strong intense signal on T 1 compared to the unlabeled cells.

Conclusions

The results showed that the novel contrast agent could become a useful tool in early detection of cancer.

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Acknowledgments

We acknowledge financial support from the Research council of Tehran University of Medical Science. We thank Mr. Behroz Rafiei for his valuable help with the 3T MRI scanner.

Conflict of Interest

All authors declare that they have no conflicts of interest associated with this work.

Author information

Authors and Affiliations

  1. Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran

    Bita Mehravi

  2. Department of Radiopharmacy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran

    Mehdi Shafiee Ardestani

  3. Department of Chemistry, Semnan University, Semnan, Iran

    Maryam Damercheli

  4. Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran

    Haleh Soltanghoraee

  5. Department of Biological Science, School of Science, Science and Research Branch, Islamic Azad University, Tehran, Iran

    Negar Ghanaldarlaki

  6. Cancer Research Center, Tehran University of Medical Sciences, Tehran, Iran

    Ali M. Alizadeh

  7. Research Center for Molecular and Cellular Imaging (RCMCI), Tehran University of Medical Sciences, Tehran, Iran

    Mohammad A. Oghabian

  8. School of Chemistry, University College of Science, University of Tehran, Tehran, Iran

    Maryam Shahzad Shirazi

  9. Department of Medicinal Chemistry, Faculty of Pharmacy and Drug Design and Development Research Center, Tehran University of Medical Sciences, Tehran, Iran

    Shabnam Mahernia & Massoud Amanlou

Authors
  1. Bita Mehravi
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  2. Mehdi Shafiee Ardestani
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  3. Maryam Damercheli
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  4. Haleh Soltanghoraee
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  5. Negar Ghanaldarlaki
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  6. Ali M. Alizadeh
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  7. Mohammad A. Oghabian
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  8. Maryam Shahzad Shirazi
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  9. Shabnam Mahernia
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  10. Massoud Amanlou
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Corresponding author

Correspondence to Massoud Amanlou.

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Mehravi, B., Ardestani, M.S., Damercheli, M. et al. Breast Cancer Cells Imaging By Targeting Methionine Transporters with Gadolinium-Based Nanoprobe. Mol Imaging Biol 16, 519–528 (2014). https://doi.org/10.1007/s11307-014-0718-3

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  • DOI: https://doi.org/10.1007/s11307-014-0718-3

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