Variation of cytosine methylation patterns in European beech (Fagus sylvatica L.)
- 208 Downloads
The role of epigenetic phenomena in plant adaptation is becoming widely recognized and the potential of epigenetics for forestry practice has been demonstrated as well. In this study, methylation-sensitive amplification polymorphism (MSAP) markers were investigated in 20 European beech (Fagus sylvatica L.) provenances that cover most of Europe and were planted in two climatically contrasting provenance trial plots. Correlations of cytosine-methylation patterns at five loci and overall DNA methylation with climatic conditions of the sites of population origin and budburst phenology were detected, suggesting that methylation at particular loci was influenced by the weather or photoperiod during embryogenesis or even earlier. Alternation of methylation patterns may also have been caused by genetic mutation. Frequencies of methylation patterns at three loci differed between the two trial locations, indicating that a climatically induced change of methylation during the ontogeny occurs as well. The results suggest that the rules for collection, transfer, and use of forest reproductive materials should also consider epigenetic effects.
KeywordsEpigenetics MSAP Fagus sylvatica Provenance trial Association studies
The provenance experiment has been established through the realization of the project European Network for the Evaluation of the Genetic Resources of Beech for Appropriate Use in Sustainable Forestry Management (AIR3-CT94-2091) under the coordination of H.-J. Muhs and G. von Wühlisch. The experimental plots Tále and Zbraslav were established by L. Paule and V. Hynek, respectively. The study was supported by a research grant of the Slovak Research and Development Agency APVV-0135-12 and the project of the Ministry of Agriculture of the Czech Republic—Resolution RO0117 (reference number 6779/2017-MZE-14151.).We also thank to K. Willingham for linguistic correction.
Data Archiving Statement
MSAP profile data have been deposited at the TreeGenes Data Repository (dendrome.ucdavis.edu/treegenes/) under the accession number TGDR069.
- Bräutigam K, Vining KJ, Lafon-Placette C, Fossdal CG, Mirouze M, Marcos JG, Fluch S, Fraga MF, Guevara MÁ, Abarca D, Johnsen Ø, Maury S, Strauss SH, Campbell MM, Rohde A, Díaz-Sala C, Cervera MT (2013) Epigenetic regulation of adaptive responses of forest tree species to the environment. Ecol Evol 3:399–415. https://doi.org/10.1002/ece3.461 CrossRefPubMedPubMedCentralGoogle Scholar
- Doyle JJ, Doyle JL (1987) Isolation of DNA from fresh plant tissue. Phytochem Bull 19:11–15Google Scholar
- Dubin MJ, Zhang P, Meng D, Remigereau M-S, Osborne EJ, Casale FP, Drewe P, Kahles A, Jean G, Vilhjálmsson B, Jagoda J, Irez S, Voronin V, Song Q, Long Q, Rätsch G, Stegle O, Clark RM, Nordborg M (2015) DNA methylation in Arabidopsis has a genetic basis and shows evidence of local adaptation. eLife 4:e05255. https://doi.org/10.7554/eLife.05255 CrossRefPubMedPubMedCentralGoogle Scholar
- Feng S, Cokus SJ, Zhang X, Chen PJ, Bostick M, Goll MG, Hetzel J, Jain J, Strauss SH, Halpern ME, Ukomadu C, Sadler KC, Pradhan S, Pellegrini M, Jacobsen SE (2010) Conservation and divergence of methylation patterning in plants and animals. Proc Natl Acad Sci USA 107:8689–8694. https://doi.org/10.1073/pnas.1002720107 CrossRefPubMedPubMedCentralGoogle Scholar
- Gömöry D, Ditmarová L, Hrivnák M, Jamnická G, Kmet’ J, Krajmerová D, Kurjak D (2015) Differentiation in phenological and physiological traits in European beech (Fagus sylvatica L.). Eur J For Res 134:1075–1085Google Scholar
- Johnsen Ø (1989) Phenotypic changes in progenies of northern clones of Picea abies (L.) Karst. grown in a southern seed orchard. I. Frost hardiness in a phytotron experiment. Scand J Forest Res 4:317–330. https://doi.org/10.1080/02827588909382569
- Joost S, Bonin A, Bruford MW, Després L, Conord C, Erhardt G, Taberlet P (2007) A spatial analysis method (SAM) to detect candidate loci for selection: towards a landscape genomics approach to adaptation. Mol Ecol 16:3955–3969. https://doi.org/10.1111/j.1365-294X.2007.03442.x CrossRefPubMedGoogle Scholar
- Kawakatsu T, Huang SSC, Jupe F, Sasaki E, Schmitz RJ, Urich MA, Castanon R, Nery JR, Barragan C, He YP, Chen HM, Dubin M, Lee CR, Wang CM, Bemm F, Becker C, O'Neil R, O'Malley RC, Quarless DX, Schork NJ, Weigel D, Nordborg M, Ecker JR, 1001 Genomes Consortium (2016) Epigenomic diversity in a global collection of Arabidopsis thaliana accessions. Cell 166:492–505. https://doi.org/10.1016/j.cell.2016.06.044 CrossRefPubMedPubMedCentralGoogle Scholar
- Krajmerová D, Hrivnák M, Ditmarová Ľ, Jamnická G, Kmeť J, Kurjak D, Gömöry D (2017) Nucleotide polymorphisms associated with climate, phenology and physiological traits in European beech (Fagus sylvatica L.) New For 48:463–477 https://doi.org/10.1007/s11056-017-9573-9
- Magri D, Vendramin GG, Comps B, Dupanloup I, Geburek T, Gömöry D, Latalowa M, Litt T, Paule L, Roure JM, Tantau I, van der Knaap WO, Petit RJ, de Beaulieu J-L (2006) A new scenario for the Quaternary history of European beech populations: palaeobotanical evidence and genetic consequences. New Phytol 171:199–221Google Scholar
- Richards CL, Alonso C, Becker C et al. (2017) Ecological plant epigenetics: evidence from model and non-model species, and the way forward. bioRxiv. https://www.biorxiv.org/content/ early/2017/04/25/130708. Accessed 15 July 2017. doi: https://doi.org/10.1101/130708
- Sinkkonen L, Hugenschmidt T, Berninger P, Gaidatzis D, Mohn F, Artus-Revel CG, Zavolan M, Svoboda P, Filipowitz W (2008) MicroRNAs control de novo DNA methylation through regulation of transcriptional repressors in mouse embryonic stem cells. Nat Struct Mol Biol 15:259–267. https://doi.org/10.1038/nsmb.1391 CrossRefPubMedGoogle Scholar
- von Wühlisch G, Krusche D, Muhs HJ (1995) Variation in temperature sum requirement for flushing of beech provenances. Silvae Genet 44:343–346Google Scholar
- von Wühlisch G, Liesebach M, Muhs HJ, Stephan BR (1998) A network of international beech provenance trials. In: Turok J, Kremer A, de Vries SM (eds) First EUFORGEN meeting on social broadleaves. International Plant Genetic Resources Institute, Rome, pp 164–172Google Scholar
- Yakovlev IA, Carneros E, Lee Y, Olsen JE, Fossdal CG (2016) Transcriptional profiling of epigenetic regulators in somatic embryos during temperature induced formation of an epigenetic memory in Norway spruce. Planta 243:1237–1249. https://doi.org/10.1007/s00425-016-2484-8 CrossRefPubMedGoogle Scholar