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Transcriptome-wide profiling and expression analysis of two accessions of Paulownia australis under salt stress

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Abstract

Paulownia australis has important economic and ecological values. In this study, the morphological and physiological changes of the leaves in diploid and autotetraploid P. australis under salt stress were analyzed. To detect related genes and gain a comprehensive perspective on the molecular mechanisms underlying salt tolerance in P. australis, transcriptome-wide gene expression profiling was conducted in the leaves of the diploid and autotetraploid P. australis under control and salinity conditions, respectively. Evaluation of the responses against salinity stress revealed the superiority of autotetraploid over diploid in terms of salinity tolerance. Changes in physiological parameters in diploid P. australis (PA2) and tetraploid P. australis (PA4) plants in response to salt stress were measured. Transcriptome data revealed that many of the common unigenes which were involved in accumulation of compatible solutes, oxidative stress detoxification, ion homeostasis, and signal transduction showed significant differences between the two accessions in response to salt stress. A number of salt-responsive unigenes were identified in two accessions of P. australis under salt stress. Furthermore, the differentially expressed unigenes found to be common in both accessions may be useful genetic resources for further genetic improvement of Paulownia using transgenic approaches.

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Funding information

This work was supported by the Joint Funds of the National Natural Science Foundation of China (NSFC) (Grant No. U1204309), the Fund of the Transformation Project of the National Agricultural Scientific and Technological Achievement of China (Grant No. 2012GB2D000271), the Central Financial Forestry Science Promotion Project (Grant No. GTH [2012]01), the Fund of the Science Key Program of Department of Henan Education (Grant No. 12A220003), and the Fund of the Technology Innovation Team Project of Zhengzhou (Grant No. 121PCXTD515).

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  1. Institute of Paulownia, Henan Agricultural University, 95 Wenhua Road, Jinshui Area, Zhengzhou, 450002, Henan, People’s Republic of China

    Yanpeng Dong, Guoqiang Fan, Zhenli Zhao, Enkai Xu, Minjie Deng, Limin Wang & Suyan Niu

  2. College of Forestry, Henan Agricultural University, 95 Wenhua Road, Jinshui Area, Zhengzhou, 450002, Henan, People’s Republic of China

    Yanpeng Dong, Guoqiang Fan, Zhenli Zhao, Enkai Xu, Minjie Deng, Limin Wang & Suyan Niu

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  1. Yanpeng Dong
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  2. Guoqiang Fan
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  3. Zhenli Zhao
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  5. Minjie Deng
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Contributions

Guoqiang Fan conceived and designed the experiments, and supervised the study. Yanpeng Dong performed experiments, analyzed data, and wrote the manuscript. Yanpeng Dong revised the manuscript. Zhenli Zhao and Enkai Xu performed the experiments. Minjie Deng, Limin Wang and Suyan Niu contributed reagents or other essential material.

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Correspondence to Guoqiang Fan.

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The authors declare that they have no competing interests.

Data archiving statement

Sequence data from this article have been deposited with the National Center for Biotechnology Information Sequence Read Archive database (http://www.ncbi. nlm.nih.gov/sra) under accession no. SRP059262.

Additional information

Communicated by W.-W. Guo

Electronic supplementary material

ESM 1

The process of the de novo transcriptome assembly. (GIF 34 kb)

High-resolution image (TIFF 10478 kb)

ESM 2

Primers of quantitative RT-PCR analysis of candidate salt response genes. -f represents forward primers and -r represents reverse primers. (DOCX 17 kb)

ESM 3

Distribution of unigene lengths in the transcriptome of P. australis. The sizes of all unigenes were calculated. (GIF 256 kb)

High resolution image (TIFF 139 kb)

ESM 4

Unigene annotations based on their BLASTX hits in the Nr, KEGG, Swiss-Prot, KOG, and GO database. (XLSX 2645 kb)

ESM 5

Classification of the Karyotic Orthologous Groups (KOG) for the transcriptome of P. australis (GIF 16 kb)

High-resolution image (TIFF 258 kb)

ESM 6

Classification of the gene ontology (GO) for the transcriptome of P. australis (GIF 1439 kb)

High-resolution image (TIFF 1156 kb)

ESM 7

KEGG annotation of unigenes (XLSX 121 kb)

ESM 8

Co-regulated differentially expressed unigenes in both the PA2T vs. PA2W and PA4T vs. PA4W comparisons. PA2T, 15 days salt-treated diploid; PA2W, well-watered diploid; PA4T, 15 days salt-treated tetraploid; PA4W, well-watered tetraploid. (XLSX 739 kb)

ESM 9

Co-regulated differentially expressed unigenes in both the PA4W vs. PA2W and PA4T vs. PA2T comparisons. PA4W, well-watered tetraploid; PA2W, well-watered diploid; PA4T, 15 days salt-treated tetraploid; PA2T, 15 days salt-treated diploid. (XLSX 699 kb)

ESM 10

Co-regulated differentially expressed unigenes in the four libraries. PA2T, 15 days salt-treated diploid; PA2W, well-watered diploid; PA4T, 15 days salt-treated tetraploid; PA4W, well-watered tetraploid. (XLSX 167 kb)

ESM 11

DEUs function in the accumulation of compatible solutes, oxidative stress detoxification, ion homeostasis and salt stress in the discussion section. (XLSX 130 kb)

ESM 12

KEGG pathway analysis results for the co-regulated differentially expressed unigenes in the four libraries.PA2T, 15 days salt-treated diploid; PA2W, well-watered diploid; PA4T, 15 days salt-treated tetraploid; PA4W, well-watered tetraploid. (XLSX 15 kb)

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Dong, Y., Fan, G., Zhao, Z. et al. Transcriptome-wide profiling and expression analysis of two accessions of Paulownia australis under salt stress. Tree Genetics & Genomes 13, 97 (2017). https://doi.org/10.1007/s11295-017-1179-z

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