Identification of the SRO gene family in apples (Malus×domestica) with a functional characterization of MdRCD1
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RCD1 is a member of the plant-specific SRO protein family. Several SRO genes have been functionally identified in the regulation of abiotic stresses in Arabidopsis and other plant species. However, the function of SROs is largely unknown in apple (Malus×domestica). In this study, six MdSRO-encoding genes were isolated, categorized into two types and mapped to six chromosomes. The phylogenetic analysis demonstrated that the sequences of the AtSRO and MdSRO proteins are highly conserved. Subsequently, expression analysis showed that MdSRO genes had different expression profiles in different tissues and in response to various stresses. Finally, MdRCD1 was isolated for functional identification. The results showed that resistance to oxidation stress in apple calli was enhanced by MdRCD1 overexpression and weakened by MdRCD1 suppression. MdRCD1 also played a crucial role in the regulation of ROS homeostasis in transgenic apple calli and Arabidopsis. Ectopic expression of MdRCD1 significantly enhanced resistance to salt and oxidative stresses in transgenic lines. In addition, MdRCD1 also enhanced drought tolerance due to its influence on stomatal opening. Based on these results, we conclude that MdRCD1 is an important regulator in abiotic stress response.
KeywordsApple SRO RCD1 Abiotic stresses
This research was supported by grants from NSFC (31430074 and 31601742), Ministry of Education of China (IRT15R42), and Shandong Province (SDAIT-06- 03).
Conceived and designed the experiments: CXY XFW HHL. Performed the experiments: HHL RL. Analyzed the data: HHL RL FJQ. Contributed reagents/materials/analysis tools: YJH JFY. Wrote the paper: HHL XFW.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
Data archiving statement
The apple SRO gene family sequences have been submitted to Genome Database for Rosaceae (GDR) (https://www.rosaceae.org/), and the accession number been shown in the Table 1. All sequences of A. thaliana SRO genes are available in the Arabidopsis Information Resource (TAIR) (https://www.arabidopsis.org/index.jsp)
- Ahlfors R, Lang S, Overmyer K, Jaspers P, Brosche M, Tauriainen A, Kollist H, Tuominen H, Belles-Boix E, Piippo M, Inze D, Palva T, Kangasjarvi J (2004) Arabidopsis RADICAL-INDUCED CELL DEATH1 belongs to the WWE protein-protein interaction domain protein family and modulates abscisic acid, ethylene, and methyl jasmonate responses. Plant Cell 16:1925–1937CrossRefPubMedPubMedCentralGoogle Scholar
- Ahlfors R, Brosché M, Kollist H, Kangasjärvi J (2009) Nitric oxide modulates ozone‐induced cell death, hormone biosynthesis and gene expression in Arabidopsis thaliana. Plant J 58(1):1–12Google Scholar
- Jaspers P, Blomster T, Brosché M, Salojärvi J, Ahlfors R, Vainonen JP, Reddy RA, Immink R, Angenent G, Turck F, Overmyer K, Kangasjärvi J (2009) Unequally redundant RCD1 and SRO1 mediate stress and developmental responses and interact with transcription factors. Plant J 60(2):268–279Google Scholar
- Kjaersgaard T, Jensen MK, Christiansen MW, Gregersen P, Kragelund BB, Skriver K (2011) Senescence-associated barley NAC (NAM, ATAF1, 2, CUC) transcription factor interacts with radical-induced cell death 1 through a disordered regulatory domain. J Biol Chem 286(41):35418–35429CrossRefPubMedPubMedCentralGoogle Scholar
- Overmyer K, Tuominen H, Kettunen R, Betz C, Langebartels C, Sandermann H, Kangasjärvi J (2000) Ozone-sensitive Arabidopsis rcd1 mutant reveals opposite roles for ethylene and jasmonate signaling pathways in regulating superoxide-dependent cell death. Plant Cell 12(10):1849–1862Google Scholar
- Petrov VD, Van Breusegem F (2012) Hydrogen peroxide—a central hub for information flow in plant cells. AoB plants pls014Google Scholar
- Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) Mega6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30(4):2725e2729Google Scholar
- Tang ZC (1999) Modern experiment procotols in plant physiologyGoogle Scholar
- Wardhan V, Jahan K, Gupta S, Chennareddy S, Datta A, Chakraborty S, Chakraborty N (2012) Overexpression of CaTLP1, a putative transcription factor in chickpea (Cicer arietinum L.), promotes stress tolerance. Plant Mol Biol 79(4-5):479–493Google Scholar
- You J, Zong W, Li X, Ning J, Hu H, Li X, Xiao J, Xiong L (2013) The SNAC1-targeted gene OsSRO1c modulates stomatal closure and oxidative stress tolerance by regulating hydrogen peroxide in rice. J Exp Bot 64(2):569–583Google Scholar