Tree Genetics & Genomes

, 13:89 | Cite as

Construction and characterization of a bacterial artificial chromosome library for Camellia sinensis

  • Yuling Tai
  • Hao Wang
  • Chaoling Wei
  • Ling Su
  • Min Li
  • Liqun Wang
  • Zhaozhao Dai
  • Lingyun Shao
  • Jiayue Jiang
  • Meizhong Luo
  • Hua Yang
  • Weiwei Deng
  • Bei Huang
  • Xiaochun WanEmail author
  • Jun SunEmail author
Original Article
Part of the following topical collections:
  1. Genome Biology


Tea is a popular and natural non-alcoholic beverage, and is produced from fresh leaves of Camellia sinensis. Tea leaves contain many bioactive compounds that have significant health benefits. We constructed a high quality bacterial artificial chromosome (BAC) library by using the fresh petals of C. sinensis “Shuchazao” for genome sequencing and improvement of genomic assembly. BAC library is still a significant tool for studies of functional genomes and preservation of precious genetic resources. The BAC library contains 161,280 clones with an average insert size of 113 kb, which represents approximately 6.2-fold coverage of haploid genome equivalents of C. sinensis. We characterized 20 complete BAC clones and 738 BAC end sequences (BESs) ranging from 105 to 917 bp. In addition, we predicted cis-regulatory elements of LAR (leucoanthocyanidin reductase), TCS (caffeine synthase), and TS (theanine synthetase) involved in tea characteristic metabolite synthesis and identified a larger number of light-responsive cis-acting elements in these three genes. Meanwhile, we analyzed alternative splicing of these three genes. Furthermore, 12 pairs of SSR primers were successfully amplified in tea plant DNA. The tea BAC library was a critical resource to accomplish de novo whole-genome sequencing, accelerate gene discovery and enhance molecular breeding of C. sinensis.


Tea plant BAC library BAC end sequencing SSR Alternative splicing 



This work received financial support from the Science and Technology Project of AnHui Province, China (Project 13Z03012), Tea Genome Project of AnHui Province, China, the Special Innovative Province Construction in Anhui province in 2015 (15czs08032), the Central Guiding the Science and Technology Development of the Local (2016080503B024), the Major Project of Chinese National Programs for Fundamental Research and Development (2012CB722903), the Natural Science Foundation of Anhui Province (No.1608085QC60), and the Youth Foundation of Anhui Agricultural University (2016ZR012). We appreciate Chun Liu (Beijing Genome Institute at Shenzhen, China) for technical support and analysis. We are grateful to the unknown editor at the elixigen editing service (ID151023-6181) for the English polishing.

Authors’ contributions

Conceived and designed the experiment: WC and SJ; analyzing data: WL, WH, TL, JY, LZ, YH, and DW; experiment: SL, DZ, SY, HB, WQ, and LM. TL and WH contributed to writing the text.

Compliance with ethical standards

Competing interests

The authors declare that they have no competing interests.

Supplementary material

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Supplementary Figures S1 (DOCX 72 kb)
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Supplementary Figures S2 (DOCX 498 kb)
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Supplementary Figures S3 (DOCX 93 kb)
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Supplementary Figures S4 (DOCX 75 kb)
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ESM 1 (XLSX 180 kb)


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Copyright information

© Springer-Verlag GmbH Germany 2017

Authors and Affiliations

  • Yuling Tai
    • 1
    • 2
  • Hao Wang
    • 3
  • Chaoling Wei
    • 1
  • Ling Su
    • 2
  • Min Li
    • 1
  • Liqun Wang
    • 1
  • Zhaozhao Dai
    • 3
  • Lingyun Shao
    • 3
  • Jiayue Jiang
    • 1
  • Meizhong Luo
    • 3
  • Hua Yang
    • 1
  • Weiwei Deng
    • 1
  • Bei Huang
    • 1
  • Xiaochun Wan
    • 1
    Email author
  • Jun Sun
    • 1
    • 4
    Email author
  1. 1.State Key Laboratory of Tea Plant Biology and UtilizationAnhui Agricultural UniversityHefeiChina
  2. 2.School of Life ScienceAnhui Agricultural UniversityHefeiChina
  3. 3.College of Life Sciences and TechnologyHuazhong Agricultural UniversityWuhanChina
  4. 4.College of HorticultureAnhui Agricultural UniversityHefeiChina

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