The complete peach dehydrin family: characterization of three recently recognized genes
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Three genes encoding dehydrins have been previously described from peach. In the course of searching the peach genome, three additional members of this stress-associated family were recognized, PpDhn4-6. PpDhn1 and 6 have no introns, whereas the remaining four genes have a single intron located near the 3′ end of the serine (S) tract. PpDHN2 was the only dehydrin with a predicted basic pI; pI predictions for the other dehydrins ranged from about 5.3 to about 6.3. None of the peach dehydrins have tryptophan residues, but, in contrast to most dehydrins, three (PpDHN1, 3, and 4) have one or more cysteine residues. Phylogenetic analysis indicated a close relationship between PpDhn1 and 6 and PpDhn3 and 4. Expression analysis under low temperature and dehydration confirmed that PpDhn2 is the major responder to drought, while both PpDhn1 and 6 respond exclusively to cold. Comparison of the first 500 base pairs upstream of the translation start site revealed the presence of cis-elements associated with low temperature and drought/osmotic/salt and hormone response regulation.
KeywordsAbiotic stress Gene expression Promoter comparison Woody plants
We appreciate the expert technical assistance of Ms. Jing Ma in the expression studies. This work was supported in its entirety by the USDA, the Agricultural Research Service, CRIS project 8080-21000-022-00D.
Data archiving statement
The genes described herein can be found at the National Center for Biotechnology Information or the Genome Database for Rosaceae as follows: PpDhn 4: GenBank Accession No. XM_007200796, GDR Locus No. ppa026861m; PpDhn5: GenBank Accession No. XM_007201751, GDR Locus No. ppa010975m; and PpDhn6: GenBank Accession No. XM_007202386, GDR Locus No. ppa009997m. Promoter regions can be found under GenBank Accession numbers as follows: PpDhn1: AY819770, PpDhn2: AY819770, PpDhn3: EU286278; use 500 bases upstream of GDR scaffold_8:16894027…16894705 (− strand) for PpDhn4; scaffold_8:11638043…11641172 (− strand) for PpDhn5; and scaffold_7:17137970…17139156 (+ strand) for PpDhn6.
GenBank accession numbers for the grape dehydrin sequences are as follows: XM03631828 (VvDHN1a), XM022285883 (VvDHN2), CAN73166 (VvDHN3), and XM002285369 (VvDHN4). Arabidopsis dehydrin GenBank gene ID numbers for the polypeptides identified by gene name are as follows: At3g50980 (Xero1), At3g50970 (Xero2), At5g66400 (Rab18), At1g20450 (ERD10), At1g76180 (ERD14), and At1g20440 (COR47).
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