Hybrid seedling inviability locus (HIs1) mapped on linkage group 4 of the Japanese flowering cherry, Cerasus × yedoensis ‘Somei-yoshino’
- 313 Downloads
Cerasus × yedoensis ‘Somei-yoshino’ (2n = 16), one of the most popular cultivars of Japanese flowering cherry, is considered a hybrid between Cerasus spachiana and Cerasus speciosa. In the present study, we observed segregation of the trait for seedling growth in F 1 progenies of Somei-yoshino. In all three families crossed with ancestral wild species, C. spachiana, approximately half of the seedlings showed growth failure. To identify loci involved in seedling inviability, we constructed a genetic linkage map of Somei-yoshino. Both normal growth (N = 77) and growth failure seedlings (N = 101) of an F 1 progenies crossed between Somei-yoshino (CY) and C. spachiana (E750) were used for map construction. In accordance to pseudo-testcross strategy, female (CY) and male (E750) linkage maps were constructed. The CY map consists of eight linkage groups with 59 simple sequence repeat (SSR) and 17 amplified fragment length polymorphism (AFLP) markers. The map length is 574.9 cM. In contrast, the E750 map reached 196.8 cM and included 17 SSRs. Subsequently, we explored positions of loci associated to seedling inviability along with linkage maps. Results of segregation distortion of markers, association analysis, and interval mapping consistently showed that an inviability gene was located between two SSR markers, EMPaS13 and BPPCT005 in the fourth linkage group (LG4) of the Somei-yoshino map with 99.6 % explanations for the inviability trait but not present in LG4 of the E750 map. From the genotypes and expressions of seedling inviability, we named the locus Hybrid Inviability of seedlings 1 (HIs1). Finally, HIs1 was mapped to LG4 as Mendelian trait loci and was completely linked with an SSR, EMPaS13. Although the mechanisms of seedling inviability cannot be explained by only one locus model of HIs1, one of the candidate genes involved in hybrid incompatibility between Somei-yoshino and C. spachiana is certainly located on LG4 of the CY map.
KeywordsFlowering cherry Hybrid inviability Linkage map Segregation distortion Somei-yoshino
This work was supported by the Sasakawa Scientific Research Grant from The Japan Science Society.
Data archiving statement
Genetic linkage maps of Somei-yoshino (CY) and C. spachiana (E750) are currently being prepared for submitting to Genome Database for Rosaceae (www.rosaceae.org).
- Dirlewanger E, Cosson P, Tavaud M, Aranzana MJ, Poizat C, Zanetto A, Arús A, Laigret F (2002) Development of microsatellite markers in peach [Prunus persica (L.) Batsch] and their use in genetic diversity analysis in peach and sweet cherry (Prunus avium L.). Theor Appl Genet 105:127–138CrossRefPubMedGoogle Scholar
- Flower Association of Japan (1982) Manual of Japanese flowering cherries. Flower association of Japan, TokyoGoogle Scholar
- Oginuma K (1977) Karyomorphological speculation on the origin of Prunus × yedoensis ‘Yedoensis’. Bull Biol Soc Hiroshima Univ 43:53–55 (in Japanese with English summary) Google Scholar
- Oginuma K, Tanaka R (1976) Karyomorphological studies on some cherry trees in Japan. J Japan Bot 51:104–109Google Scholar
- Ohba H, Kawasaki T, Tanaka H (2007) Flowering cherries of Japan, Newth edn. Yama-kei Publishers, Tokyo (in Japanese) Google Scholar
- Olmstead JW, Sebolt AM, Cabrera A, Sooriyapathirana SS, Hammar S, Iriarte G, Wang D, Chen CY, van der Knaap E, Iezzoni AF (2008) Construction of an intra-specific sweet cherry (Prunus avium L.) genetic linkage map and synteny analysis with the Prunus reference map. Tree Genet Genome 4:897–910CrossRefGoogle Scholar
- Verde I, Abbott AG, Scalabrin S, Jung S, Shu S, Marroni F, Zhebentyayeva T, Dettori MT, Grimwood J, Cattonaro F et al (2013) The high-quality draft genome of peach (Prunus persica) identifies unique patterns of genetic diversity, domestication and genome evolution. Nat Genet 45:487–494CrossRefPubMedGoogle Scholar
- Wang S, Basten CJ, Zeng ZB (2012) Windows QTL Cartographer 2.5. Department of Statistics, North Carolina State University, Raleigh, NCGoogle Scholar
- Wilson EH (1916) The cherries of Japan. University press, CambridgeGoogle Scholar