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Heteroallelic diploid pollen led to self-compatibility in tetraploid cultivar ‘Sha 01’ (Pyrus sinkiangensis Yü)

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Abstract

Pear cultivar ‘Sha 01’ is a sport from ‘Kuerlexiangli’. Field pollination data revealed that ‘Sha 01’ displayed self-compatibility (SC), whereas ‘Kuerlexiangli’ showed self-incompatibility upon self-pollination. Reciprocal pollinations between the two varieties showed that 76% of ‘Kuerlexiangli’ flowers pollinated with ‘Sha 01’ pollen set fruit, but only 7% of ‘Sha 01’ flowers set fruit when pollinated with ‘Kuerlexiangli’ pollen. The pollen performance was monitored with fluorescence microscopy, and it was observed that ‘Sha 01’ accepted self-pollen but rejected ‘Kuerlexiangli’ pollen, whereas ‘Kuerlexiangli’ rejected self-pollen but accepted ‘Sha 01’ pollen. Taken together, ‘Sha 01’ showed SC as a pollen-part mutant. Molecular S-genotyping of ‘Sha 01’, its selfed progeny, and wild-type ‘Kuerlexiangli’ showed that all contained S 22-RNase and S 28-RNase alleles, but showed no nucleotide sequence difference or changes in transcription. After flow cytometry and chromosome number analyses, ‘Sha 01’ was found to be a tetraploid (2n = 68) and ‘Kuerlexiangli’ a diploid (2n = 34). Thus, the S-genotype for ‘Sha 01’ was S 22 S 22 S 28 S 28 and for ‘Kuerlexiangli’ was S 22 S 28 . Ploidy level of ‘Sha 01’ selfed progeny were also determined from leaves using flow cytometry; all seedlings were tetraploids. The S-genotyping for the progeny was identified by S-RNase gene relative optical density for both alleles. Closer inspection of 52 progeny showed that they could be classified into three types S 22 S 22 S 28 S 28 : S 22 S 22 S 22 S 28 : S 22 S 28 S 28 S 28 with the distribution 28:10:14 ≈ 4:1:1; no individual homozygous for either allele was found. Therefore, it could be concluded that only heteroallelic diploid pollen S 22 S 28 could achieve ‘Sha 01’ fertilization through a so-called competitive interaction.

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Acknowledgments

This work was financially supported by the National Pear Industry Technology Research System (:29) and Natural Foundation of Jiangsu Province (BK2007164).

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Authors and Affiliations

  1. College of Horticulture, State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, Jiangsu, 210095, China

    Yong-Jie Qi, Hua-Qing Wu, Jun Wu, Shu-Tian Tao & Shao-Ling Zhang

  2. Fruit Research Institute, Chinese Academy of Agricultural Sciences, Xingcheng, Liaoning, 125100, China

    Yu-Fen Cao

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  1. Yong-Jie Qi
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Correspondence to Shao-Ling Zhang.

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Communicated by F. Gmitter

Yong-Jie Qi and Hua-Qing Wu contributed equally to this work

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Qi, YJ., Wu, HQ., Cao, YF. et al. Heteroallelic diploid pollen led to self-compatibility in tetraploid cultivar ‘Sha 01’ (Pyrus sinkiangensis Yü). Tree Genetics & Genomes 7, 685–695 (2011). https://doi.org/10.1007/s11295-011-0366-6

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